Environmental Antimicrobial Resistance: Implications for Food Safety and Public Health

Antibiotics, Journal Year: 2024, Volume and Issue: 13(11), P. 1087 - 1087

Published: Nov. 14, 2024

Antimicrobial resistance (AMR) is a serious global health issue, aggravated by antibiotic overuse and misuse in human medicine, animal care, agriculture. This study looks at the different mechanisms that drive AMR, such as environmental contamination, horizontal gene transfer, selective pressure, well severe implications of AMR for health. demonstrates need concerted efforts across scientific, healthcare, agricultural, policy sectors to control emergence AMR. Some crucial strategies discussed include developing antimicrobial stewardship (AMS) programs, encouraging targeted narrow-spectrum use, emphasizing significance strict regulatory frameworks surveillance systems, like Global Resistance Use Surveillance System (GLASS) Access, Watch, Reserve (AWaRe) classification. also emphasizes national international action plans combating promotes One Health strategy, which unifies environmental, animal, concludes preventing spread maintaining effectiveness antibiotics future generations requires comprehensive, multidisciplinary, internationally coordinated strategy.

Language: Английский

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Antibiotic resistance of Escherichia coli isolated from food and clinical environment in China from 2001 to 2020

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 939, P. 173498 - 173498

Published: May 28, 2024

Language: Английский

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Understanding the spread of antibiotic resistance in vegetables cultivated with sewage sludge: implications for food safety and human health

ENVIRONMENTAL SYSTEMS RESEARCH, Journal Year: 2024, Volume and Issue: 13(1)

Published: June 6, 2024

Abstract The conventional practice of using sewage treatment plant (STP) derived sludge as a fertilizer poses significant negative impacts on agroecosystems. Since has diverse contaminants, including heavy metals (HMs), antibiotics (ABs) and antibiotic resistance genes (ARGs), its application in the agricultural fields contaminates food hence causes human health risks via chain. transfer ABs ARGs from to soil then plants can influence development (AR) endophytes, leads variations their characteristics. In pot experiment, vegetable carrot ( Daucus carota ) spinach Spinacia oleracea were amended with samples three (STPs) varying capacities both above below-ground parts analysed for presence specific (amoxicillin, azithromycin, chloramphenicol, ciprofloxacin, tetracycline), blaCTX-M, blaGES, blaNDM, ermF, qnrS, Sul1 ), mobile genetic elements (MGEs) intl1 , IS26 ). Among characterized culturable endophytic bacteria (EB), 22 exhibited various (highest against ampicillin, chloramphenicol) lead, nickel, chromium). Most importantly, seven multiple antibiotic-resistant (MAREB) all tested (HMs). Additionally, MAREB positive biofilm production, notable proportion (72.72%) these endophytes displayed mobility, strong auto-aggregation ranging 16.67 92.61%. formation dynamics among Gaussian distribution pattern, increasing higher concentrations. Notably, five demonstrated survival at clarithromycin concentrations up 150 µg ml −1 . study revealed (µg kg (copies vegetables, 2.87 314.88 1 × 10 5 3.2 respectively. advantageous features support growth under different stress conditions. Moreover, 51.09% identified EBs reported human-associated pathogens, 9.09% solely pathogens. Transfer factor (TF), translocation (TLF), bioconcentration (BCF) values correlated abundance root shoot compartments vegetables. risk assessment highlighted children are particularly vulnerable prolonged adverse consuming Therefore, this research is imperative understanding co-selection mechanisms, need improvement existing systems contaminants removal, evaluation before fields.

Language: Английский

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Non-Canonical Aspects of Antibiotics and Antibiotic Resistance

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 565 - 565

Published: June 17, 2024

The understanding of antibiotic resistance, one the major health threats our time, is mostly based on dated and incomplete notions, especially in clinical contexts. “canonical” mechanisms action pharmacodynamics antibiotics, as well methods used to assess their activity upon bacteria, have not changed decades; same applies definition, acquisition, selective pressures, drivers resistance. As a consequence, strategies improve usage overcome resistance ultimately failed. This review gathers most “non-canonical” notions antibiotics resistance: from alternative limitations susceptibility testing wide variety lateral gene transfer mechanisms, ubiquity, societal factors maintaining Only by having “big picture” view problem can adequate harness be devised. These must global, addressing many aspects that drive increasing prevalence resistant bacteria aside use antibiotics.

Language: Английский

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Antimicrobial Resistance Gene Profiles in Communities Selected with Novel and Previously Encountered Antibiotics

Theoretical and Natural Science, Journal Year: 2025, Volume and Issue: 89(1), P. 78 - 85

Published: Jan. 15, 2025

Antibiotics are extensively employed worldwide for therapeutic purposes, often leading to overuse, which constitutes a contributing factor the emergence of antibiotic resistance (ABR). This phenomenon arises from diminished effectiveness antibiotics in treating bacterial infections due development. However, limitations experimental conditions and equipment making it challenging pinpoint precise sources resistance, consequently constraining breadth research on ABR. Genomic analysis antibiotic-resistant bacteria assists unveiling origins pathways dissemination. study employs Nanopore-based metagenomic sequencing, enabling direct sequencing microbial DNA within macrogenomic samples, thereby yielding comprehensive sequence data. Coupled with qPCR 16S rRNA gene datasets, this approach facilitates exploration distribution prevalence communities across different environments Focusing Scottish farmlands, isolated colonies were exposed (sulfamonomethoxine erythromycin) several weeks. By comparing them untreated control group microorganisms, identification genes (ABRGs), along their variations abundance, aims analyse impact these two species.

Language: Английский

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Exogenous plasmid capture to characterize tetracycline-resistance plasmids in sprouts obtained from retail in Germany

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

This study aimed to characterize antibiotic-resistance plasmids present in microorganisms from sprout samples using exogenous plasmid capture. Fresh mung bean sprouts were predominantly colonized by bacteria the phyla Proteobacteria and Bacteroidetes . To capture plasmids, a plasmid-free Escherichia ( E. ) coli CV601 strain, containing green fluorescent protein gene for selection, was used as recipient strain experiments. Transconjugants selected on media cefotaxime or tetracycline antibiotics. While no cefotaxime-resistant transconjugants obtained, 40 tetracycline-resistant isolates obtained sequenced Illumina NextSeq short read Nanopore MinION long sequencing. Sequences assembled Unicycler hybrid assembly. Most of captured carried either tet (A) (D) resistance gene, belonged IncFI IncFII replicon types, predicted conjugative. smaller contained well additional quinolone qnr S1), sulfonamide sul 1) trimethoprim dfr A1) genes, larger only gene. An exception largest 192 kbp isolated, which (D), sul1 streptomycin aad genes. The isolated different more often showed 100% identity size (71,155 bp), while 180 some differences (in between 157,683 192,360 bp). suggested that similar production batches could be clonally related. based 16S metagenomics presence Enterobacter En. cloacae , ludwigii kobei Citrobacter C. werkmanii freundii Klebsiella K. oxytoca pneumonia have previously been fresh produce Germany. These may harbor antibiotic genes potentially transferred genera. demonstrated act donors can transfer other this product via conjugation.

Language: Английский

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Intrinsic and Acquired Antimicrobial Resistomes in Plant Microbiomes: Implications for Agriculture and Public Health

Journal of Sustainable Agriculture and Environment, Journal Year: 2025, Volume and Issue: 4(1)

Published: March 1, 2025

ABSTRACT Antimicrobial resistance (AMR) poses a significant threat to both human and environmental health. Before intervention, the natural resistome existed in relatively balanced state, mainly regulated by microbial interactions factors. However, continuous use of antimicrobials other novel entities (chemicals or biological substances) agricultural production clinical settings has resulted huge release residual into environment. This may lead decrease diversity an increase selection pressure. The outcome is alteration with mobile clinically relevant antibiotic genes (ARGs), posing risk In sector, emergence AMR result multiple mechanisms. It involves intricate between activities, factors processes. Direct exposure antibiotic‐resistant bacteria ARGs produce particularly raw eaten vegetables, salad, herbs fruits facilitate spread humans review aims provide comprehensive overview fresh microbiomes. focuses on impact practices risks associated More importantly, this highlights several mitigation strategies future interventions for better understanding ARG transmission within food systems.

Language: Английский

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Occurrence and Abundance of Antibiotic Resistance Genes in Chinese Traditional Pickles

Foodborne Pathogens and Disease, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

With the widespread application and even misuse of antibiotics, antibiotic resistance genes (ARGs) are extensively present in various environments, from natural environment to fermented foods, posing emerging threat public environmental health. The real-time fluorescence quantitative PCR (qPCR) technique is commonly used detect ARGs samples such as soil or water. In this study, eight types pickles were collected four regions China existence 13 was assessed by qPCR. results showed that a total 11 detected pickles, blaTEM gene all samples, neo cat absent. abundance varied, aada1 (1.09 × 105 5.94 106 copies/g), (1.48 2.2 ermc (1.01 5.35 hyg (1.35 1.93 aadd (4.46 1.60 nat1 (1.04 5.04 nptII (2.17 104 1.69 sul1 (2.01 4.60 tetl (1.23 6.18 shble (1.68 stra (4.8 1.9 105copies/g). We also discussed specificity sensitivity assessment qPCR applied analysis verifying feasibility validity method. Bacteria isolated purified well their antimicrobial studied. This study great significance for risk pickles. Effective preventive solutions proposed reduce spread protect dietary

Language: Английский

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Cessation of manure application diminishes the dissemination potential of antibiotic resistance genes by altering bacterial interaction patterns in soil–lettuce systems

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 211, P. 106100 - 106100

Published: April 17, 2025

Language: Английский

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Correlation of in vitro biofilm formation capacity with persistence of antibiotic-resistant Escherichia coli on gnotobiotic lamb’s lettuce

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Bacterial contamination of fresh produce is a growing concern for food safety, as apart from human pathogens, antibiotic-resistant bacteria (ARB) can persist on leafy produce. A prominent persistence trait in biofilm formation, it provides increased tolerance to stressful conditions. We screened comprehensive collection 174 antibiotic-susceptible and -resistant Escherichia coli originating its production environment. tested the ability these strains biofilms, ranging none or weak extreme biofilm-forming bacteria. Next, we selected isolates colonize gnotobiotic lamb’s lettuce ( Valerianella locusta ) plants. hypothesized that higher vitro formation capacity correlates with colonization plant leaves. Despite marked difference form biofilms number E. strains, was not associated survival V. leaf surfaces. However, all persisted at least 21 days, highlighting potential safety risks through unwanted ingestion resistant Population densities exhibited complex pattern, subpopulations more successful colonizing These findings emphasize behavior ARB surfaces their implications safety. IMPORTANCE Each raw contains microorganisms, including This special importance such salads herbs, foods are usually consumed after minimal processing, whereby loads living ingested than heated before consumption. common bacterial lifestyle involves large groups embedded secreted protective substances. Such assemblies, so-called confer high resistance external harsh In our research, investigated whether stronger by better Although no clear correlation observed between population density salad, could survive 3 weeks significant decline over time, risk independently formation.

Language: Английский

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