Neglected class A carbapenemases: Systematic review of IMI/NmcA and FRI from a One Health perspective

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 959, P. 178300 - 178300

Published: Jan. 1, 2025

Language: Английский

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Opportunistic Gulls Infected by Antibiotic‐Resistant Bacteria Show Contrasting Movement Behaviour

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

ABSTRACT The emergence, spread and potential zoonotic importance of pathogenic‐resistant bacteria (e.g., Escherichia coli ) has fuelled the research on epidemiology vector movement dynamics. However, little is known about effects that apparently asymptomatic carriage may have host behaviour. Here, we analysed compared patterns habitat use (focused different risk exposure to Antibiotic Resistance) yellow‐legged gulls ( Larus michahellis carrying n = 10) not 29) Antibiotic‐resistant . Using data from GPS devices coupled with accelerometers, found evidence individuals resistant E. , although previously considered asymptomatic, had lower accumulated travelled distances moved over smaller areas. resistance affect some extent, as in this case, potentially reducing pathogen dispersal large

Language: Английский

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Unravelling urban nesting site selection in an opportunistic gull: an integrated analysis of micro-spatial habitat and litter quantification

European Journal of Wildlife Research, Journal Year: 2024, Volume and Issue: 70(4)

Published: June 28, 2024

Abstract The proliferation of gull populations in urban areas has led to an increase human-gull conflicts, especially highly populated cities where these opportunistic predators are often considered a nuisance. There is lack data regarding the selection nesting sites by gulls, so management measures at level cannot be implemented. Here, we investigated main environmental factors that explain preferences yellow-legged ( Larus michahellis ) areas, using population city Barcelona (NE Spain) as study model. We conducted integrated analysis combines micro-scale habitat assessments 148 with macro-scale assessment movements derived from GPS tracking breeding gulls. also analysed type and abundance litter nests related this. Nests were mainly found on flat roofs or above structure roof located corner placements situated gravel substrate. Nest showed negative relation port distance building height beyond 12 m. presence was detected more than 80% density. Understanding ecology gulls implications for prevent nest establishment.

Language: Английский

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Evil and allies: Opportunistic gulls as both spreaders and sentinels of antibiotic‐resistant bacteria in human‐transformed landscapes

Journal of Applied Ecology, Journal Year: 2024, Volume and Issue: 61(11), P. 2809 - 2821

Published: Oct. 1, 2024

Abstract Human‐transformed residuals, especially those derived from human waste (dumps), farmland, and livestock are involved in the emergence of antibiotic‐resistant bacteria (ARB) environment. Wildlife can act as vectors ARB dispersal through different environments, but also sentinels to detect early spread determine sources. The development integrated monitoring programmes focused on wildlife would help anticipate risks humans livestock. We used yellow‐legged gull ( Larus michahellis ) a model species investigate monitor spatial patterns across an extensive farmland region located northeastern Spain (Lleida). By integrating GPS tracking data clinical testing for 26 individuals within network analysis framework, we modelled risk pathogen faeces during bacteria‐transmission latency period (16 days after sample collection). Additionally, created connectivity main sources area, focusing three habitats special infection: dumps, facilities, irrigation ponds. Seven were infected by Escherichia coli , with one co‐infected Listeria monocytogenes Salmonella spp. Potential distances ranged 1.13 km 23.13 breeding colony. Our analyses revealed 54 nodes (i.e. high‐risk recurrently visited tracked gulls) 1182 links among them. findings high degree between shallow lake, nearby highlighting them significant contributors dispersal. Synthesis applications : integration data, shed further light dynamics creating maps identifying In combination complementary molecular epidemiology techniques One Health our approach emerge important tool highly human‐transformed ecosystems. This may empower managers targeted effective mitigation strategies, ultimately improving both animal public health.

Language: Английский

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Urban yellow-legged gull (Larus michahellis) and peri-urban Audouin's gull (Larus audouinii) as a source of Campylobacter and Salmonella of public health relevance

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 960, P. 178227 - 178227

Published: Jan. 1, 2025

Campylobacter spp. and Salmonella are the leading cause of human enteric infections in European Union. Some gull species act as reservoirs play an important role epidemiology these zoonotic agents. To gain insight into we studied colonies Audouin's (Larus audouinii) yellow-legged michahellis) Barcelona metropolitan area, Catalonia (north-eastern Spain). We assessed occurrence, genetic diversity, virulence potential, antimicrobial susceptibility isolates recovered from faeces different time periods within 2009-2018. The occurrence was higher compared to both species. Also, pathogens significantly (45 % for Campylobacter, 20 Salmonella), than (13 7 %, respectively). All but one individual carried C. jejuni; remaining positive lari. serovar Typhimurium (including its monophasic variant) most frequent hosts followed by ser. Bredeney. Other serovars frequently associated with salmonellosis (Infantis, London, Virchow) were only isolated gulls. Multilocus Sequence Typing analyses showed that not several genotypes enteritis. revealed a high opposed which lower prevalence virulence-associated genes, particularly gull. Overall, moderate frequency resistance multidrug resistance) found resistant antimicrobials relevance medicine. our results highlight potential public health threat species, gull, densely populated areas.

Language: Английский

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An Unusual ’Gift’ from Humans: Third-Generation Cephalosporin-Resistant Enterobacterales in migratory birds along the East Asian-Australasian Flyway harboring widespread resistant plasmids

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109320 - 109320

Published: Feb. 12, 2025

Migratory birds play a pivotal role in the global dissemination of antimicrobial resistance genes (ARGs), with shorebirds relying on coastal wetlands during their long-distance migrations, environments often contaminated and conducive to ARG transmission. However, systematic investigations into (AMR) remain scarce. During spring autumn 2023, we collected 893 throat cloacal swabs from 480 shorebirds, representing 28 species, at Chongming Dongtan, critical stopover along East Asian-Australasian Flyway. Our analysis identified six strains, including four extra-intestinal pathogenic E. coli (ExPEC) two K. pneumoniae, that exhibited third-generation cephalosporins, three ExPEC strains exhibiting significant virulence Galleria mellonella infection assays. We conjugative plasmids: E042113F_p1, carrying blaCMY-2 gene coli, M50_p2, blaKPC-2 hypervirulent pneumoniae plasmid harboring aerobactin system. Bioinformatic experimental analyses confirmed these plasmids could transfer without any fitness cost, remaining stable for least 30 passages. Surprisingly, genomic tracing revealed among similar E042113F_p1 (blaCMY-2), earliest was Chinese swallow 2015, subsequent detections wild Mongolia (2017), Russia (2018), Australia (2019). Notably, E04-CMY-like/M50-KPC-like predominantly originated human sources, underscoring activity cross-species transmission AMR. This human-mediated elements wildlife posed substantial risk amplifying disseminating AMR through long-range migratory bird movements, highlighting urgent need international collaboration under One Health framework. Integrated surveillance, environmental management, stringent antibiotic stewardship are mitigating risks by spreading across ecosystems.

Language: Английский

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Sources of persistent organic pollutants and their physiological effects on opportunistic urban gulls

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133129 - 133129

Published: Dec. 1, 2023

Urbanization is associated with drastic shifts in biodiversity. While some species thrive urban areas, the impact of inhabiting these human-altered environments on organism physiology remains understudied. We investigated how exposure to polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) affects yellow-legged gulls (Larus michahellis) a densely populated, industrialized city. analyzed blood samples from 50 (20 immatures 30 adults) assessed 27 physiological parameters biomarkers related xenobiotic protection, health, feeding habits same individuals. also tracked movements 25 (15 10 identify potential sources persistent organic pollutants (POPs). Both adult immature primarily inhabited followed by marine habitats. Immature spent more time freshwater, landfills, agricultural areas. Bioaccumulated ΣPCB median = 92.7 ng g-1 ww (1.86–592) ΣPBDE 1.44 (0.022–9.58) showed no significant differences between age sex groups. Notably, males exhibited highest correlations POP concentrations, particularly activity carboxylesterases (CEs), suggesting higher sensitivity than adults. These findings highlight plasmatic CEs as effective tracers POPs effects, offering insights into anthropogenic impacts This manuscript addresses study environmentally relevant pollutants, specifically (PCBs, 7 congeners) (PBDEs, congeners), their gulls' physiology. are well-known for persistence environment, posing threat long-lived predators due capacity bioaccumulate. Furthermore, this it was conducted populated area, where both humans wildlife co-exist. Therefore, accordance One Health approach, could serve sentinel assessing pollutant cities.

Language: Английский

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Variability of faecal microbiota and antibiotic resistance genes in flocks of migratory gulls and comparison with the surrounding environment

Environmental Pollution, Journal Year: 2024, Volume and Issue: 359, P. 124563 - 124563

Published: July 15, 2024

Gulls commonly rely on human-generated waste as their primary food source, contributing to the spread of antibiotic-resistant bacteria and resistance genes, both locally globally. Our understanding this process remains incomplete, particularly in relation its potential interaction with surrounding soil water. We studied lesser black-backed gull, Larus fuscus, a model examine spatial variation faecal bacterial communities, antibiotic genes (ARGs), mobile genetic elements (MGEs) relationship water soil. conducted sampling campaigns within connectivity network different flocks gulls moving across functional units (FUs), each which represents module highly interconnected patches habitats used for roosting feeding. The FUs vary habitat use, some using more polluted sites (notably landfills), while others prefer natural environments (e.g., wetlands or beaches). Faecal communities from that visit spend time landfills exhibited higher richness diversity. microbiota showed high compositional overlap was greater when compared landfill (11%) than wetland soils (6%), much lower (2% 1% water, respectively). relative abundance ARGs MGEs were similar between FUs, variations observed only specific families MGEs. When exploring carriage bird faeces compartments, gull enriched classified High-Risk. results shed light complex dynamics wild populations, providing insights into interactions among movement feeding behavior, characteristics, dissemination determinants environmental reservoirs.

Language: Английский

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Changing dynamics of antibiotic resistant Escherichia in Caspian gulls shows the importance of longitudinal environmental studies

Environment International, Journal Year: 2024, Volume and Issue: 186, P. 108606 - 108606

Published: March 26, 2024

This study is focused on Escherichia spp. isolates resistant to critically important antibiotics (cefotaxime, ciprofloxacin and colistin) among Caspian gull's (Larus cachinnans) chicks nesting in the Nove Mlyny Water Reservoir, Czech Republic. The prevalence of antimicrobial resistance (AMR) bacteria within wild birds commonly evaluated using a single sampling event, capturing only brief momentary snapshot at particular location. Therefore, gulls our were sampled May 2018 (n = 72) 2019 45), water sample was taken from reservoir (2019). We obtained 197 identified as E. coli by MALDI-TOF MS. A total 158 representative whole-genome sequenced, 17 then reclassified albertii. observed higher (86 %; 62/72) occurrence ESBL/AmpC-producing compared 38 % (17/45) (p < 0.00001). decrease linked clonal lineage ST11893 predominating which carried bla

Language: Английский

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Diversity of Culturable Yeasts in the Feces of Mew Gulls Breeding in Natural and Urban Habitats, with Insights into the Antifungal Susceptibility of the Observed Pathogens

Birds, Journal Year: 2024, Volume and Issue: 5(3), P. 543 - 557

Published: Aug. 22, 2024

Migratory birds play an important role in the spread of yeasts environment over long distances and different geographical regions. Human activities, turn, have a major impact on biology wild and, consequently, microbial communities for which act as carriers disseminators. We sought to assess “response” diversity culturable feces Mew Gulls type nesting site (natural/anthropogenic) during breeding season from April October 2023. isolated molecularly identified 26 yeast species. The species composition natural habitat was more diverse, increased October. In contrast, urban decreased Analysis susceptibility conventional antibiotics (fluconazole, voriconazole, amphotericin B) using CLSI BMD (Clinical Laboratory Standards Institute broth microdilution) method strains opportunistic Candida (C. parapsilosis C. tropicalis) emerging pathogen Rhodotorula mucilaginosa showed that proportion resistant higher anthropogenic population. spent their near landfill flew away wintering appear be source pathogenic with resistance against antifungal agents.

Language: Английский

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