Luteolin enhanced antioxidant capability and induced pyroptosis through NF-κB/NLRP3/Caspase-1 in splenic lymphocytes exposure to ammonia

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170699 - 170699

Published: Feb. 5, 2024

Language: Английский

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Se Alleviated Pb-Caused Neurotoxicity in Chickens: SPS2-GPx1-GSH-IL-2/IL-17-NO Pathway, Selenoprotein Suppression, Oxidative Stress, and Inflammatory Injury

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 370 - 370

Published: March 18, 2024

Lead (Pb), a heavy metal environmental pollutant, poses threat to the health of humans and birds. Inflammation is one most common pathological phenomena in case illness poisoning. However, underlying mechanisms inflammation remain unclear. The cerebellum thalamus are important parts nervous system. To date, there have been no reports Pb inducing animal cerebellums or thalami. Selenium (Se) can relieve Therefore, we aimed explore mechanism by which Se alleviates toxicity thalami chickens establishing chicken or/and treatment model. Our results demonstrated that exposure caused inflammatory damage thalami, evidenced characteristics inflammation, decrease anti-inflammatory factors (interleukin (IL)-2 interferon-γ (INF-γ)), increase pro-inflammatory (IL-4, IL-6, IL-12β, IL-17, nitric oxide (NO)). Moreover, found IL-2/IL-17–NO pathway took part Pb-caused injury. above findings were reversed supplementation dietary Se, meaning relieved via pathway. In addition, an up-regulated oxidative index malondialdehyde (MDA) two down-regulated antioxidant indices (glutathione (GSH) total capacity (TAC)) recorded after received stimulation, indicating excess oxidant/antioxidant imbalance stress, stress mediated GSH–IL-2 axis. Interestingly, inhibited four glutathione peroxidase (GPx) family members (GPx1, GPx2, GPx3, GPx4), three deiodinase (Dio) (Dio1, Dio2, Dio3), fifteen other selenoproteins (selenophosphate synthetase 2 (SPS2), selenoprotein (Sel)H, SelI, SelK, SelM, SelO, SelP1, SelPb, SelS, SelT, SelU, (Sep)n1, Sepw1, Sepx1, Sep15), suggesting reduced resulted involving SPS2–GPx1–GSH supplementation, as expected, changes mentioned above, improved mitigated chickens. For first time, discovered SPS2–GPx1–GSH–IL-2/IL-17–NO involved complex conclusion, this study Pb-induced This offers novel insights into pollutant-caused poisoning provides theoretical basis for detoxification effect against toxic pollutants.

Language: Английский

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The mechanism of selenium regulating the permeability of vascular endothelial cells through selenoprotein O

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103063 - 103063

Published: Feb. 2, 2024

Vascular diseases, a leading cause of death in human, are strongly associated with pathological damage to blood vessels. The selenoprotein (Sel) have been reported play important roles vascular disease. However, the role SelO disease has not conclusively investigated. present experiment was investigate regulatory mechanism effect on permeability endothelial. H.E staining, FITC-Dextran Dil-AC-LDL staining and FITC-WGA showed that structure damaged, intercellular junctions were disrupted selenium (Se)-deficient. Immunohistochemistry, qPCR Western blot revealed decreased expression adhesion plaque proteins vinculin, talin paxillin, connectivity effector molecules connexin, claudin-1 E-cadherin increased JAM-A N-cadherin, as well ZO-1 signaling pathways ZO-1, Rock, rhoGEF, cingulin MLC-2. In screening 24 Sel mice, most pronounced changes tissues, possible association between junction effectors determined using IBM SPSS Statistics 25. Silencing SelO, endothelial adverse effects present. relationship determined. results Se deficiency lead tissue by decreasing expression, suggesting for regulating permeability.

Language: Английский

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Decursinol angelate relieves inflammatory bowel disease by inhibiting the ROS/TXNIP/NLRP3 pathway and pyroptosis

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Despite evidence of the efficacy decursinol angelate (DA), a prescription medication derived farom traditional Chinese medicine, in alleviating inflammatory bowel disease (IBD), precise mechanisms behind its action remain unclear. Lipopolysaccharides (LPS) and dextran sodium sulfate (DSS) induction were used as vitro vivo models IBD, respectively, to assess role DA IBD. Enzyme-linked immunosorbent assay (ELISA) was performed detect expression levels pro-inflammatory cytokines mouse serum, Western blot TXNIP/NLRP3 pathway tight junction (TJ) proteins colon tissues cells, immunohistochemistry, immunofluorescence qRT-PCR validate related this signaling pathway. Molecular docking technique co-immunoprecipitation (Co-IP) method applied evaluate targeting effect on NLRP3 proteins, MCC950, specific inhibitor NLRP3, positive control for validation. Our research indicates that DA's distinctive molecular mechanism could entail binding protein, thereby suppressing activation diminishing assembly inflammasome, thus functioning an anti-inflammatory agent. may play improving BD by inhibiting ROS/TXNIP/NLRP3 release mediators, repairing intestinal barrier function.

Language: Английский

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Zearalenone induces liver injury in mice through ferroptosis pathway

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 952, P. 175875 - 175875

Published: Aug. 30, 2024

Language: Английский

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Selective detection of food contaminants using engineered gallium-organic frameworks with MD and metadynamics simulations

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 5, 2024

The exclusion mechanism of food contaminants such as bisphenol A (BPA), Flavonoids (FLA), and Goitrin (GOI) onto the novel gallium-metal organic framework (MOF) functionalized MOF with oxalamide group (MOF-OX) is evaluated by utilizing molecular dynamics (MD) Metadynamics simulations. atoms in molecules (AIM) analysis detected different types atomic interactions between contaminant substrates. To assess this procedure, a range descriptors including interaction energies, root mean square displacement, radial distribution function (RDF), density, hydrogen bond count (HB), contact numbers are examined across simulation trajectories. most important elements stability systems under examination found to be stacking π-π HB interactions. It was confirmed significant value total energy for BPA/MOF-OX (- 338.21 kJ mol

Language: Английский

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Advances of Selenium in Poultry Nutrition and Health

Journal of Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Hesperidin mitigated deoxynivalenol-induced liver injury by inhibiting ROS/ P53/ PGC-1α-mediated disruption of mitochondrial dynamics and PANoptosis

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156747 - 156747

Published: April 1, 2025

Language: Английский

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BPA exposure and Se deficiency caused spleen damage in chickens by nitrification stress-TNF-α

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 367, P. 121994 - 121994

Published: July 30, 2024

Language: Английский

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Combination treatment of zinc and selenium intervention ameliorated BPA-exposed germ cell damage in SD rats: elucidation of molecular mechanisms

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: 397(9), P. 6685 - 6704

Published: March 18, 2024

Language: Английский

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