Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment DOI Creative Commons
Zhang Li, Zhehao Shi, Fan Zhang

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: May 19, 2023

Skin cutaneous melanoma (SKCM) is the deadliest type of malignancy. Ubiquitination a process protein sorting and degradation that exhibits multiple functions in progression various tumors. This study aimed to characterize set genes for ubiquitination SKCM.The expression patterns ubiquitin-associated (URGs) corresponding clinical information SKCM tissues were comprehensively analyzed based on The Cancer Genome Atlas (TCGA) database. We performed univariate multivariate Cox proportional regression models risk scores identify four critical related prognostic (HCLS1, CORO1A, NCF1 CCRL2), which used construct signatures. also studied effects HCLS1, CORO1A CCRL2 tumor metastasis-related indicators at cellular level through vitro experiments.SKCM patients low-risk group showing longer survival than those high-risk group. Characteristic correlated with several clinicopathological variables reflected infiltration immune cells. In addition, knockdown CLS1, affected malignant biological behavior EMT signaling pathway.This provides novel prospective strategy improve patients.

Language: Английский

Peroxiredoxin 1 modulates oxidative stress resistance and cell apoptosis through stemness in liver cancer under non-thermal plasma treatment DOI Creative Commons
Yingying Hao,

Wan-Qiu Xiao,

Huina Zhang

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 738, P. 150522 - 150522

Published: Aug. 8, 2024

The role of peroxiredoxin 1 (PRDX1), a crucial enzyme that reduces reactive oxygen and nitrogen species levels in HepG2 human hepatocellular carcinoma (HCC) cells, the regulation HCC cell stemness under oxidative stress underlying mechanisms remain largely unexplored. Here, we investigated therapeutic potential non-thermal plasma targeting cancer stem cells (CSCs) HCC, focusing on resistance to PRDX1. By simulating conditions using plasma-activated medium, found reduction PRDX1 resulted considerable increase apoptosis, suggesting plays key defense CSCs. Furthermore, had higher spheroid formation capability increased markers (CD133, c-Myc, OCT-4), indicating strong stemness. Interestingly, expression was notably than other types such as Hep3B Huh7 whereas PRDX family proteins (PRDX 2–6) were relatively consistent. inhibition peroxidase activity by conoidin A markedly reduced traits death rate. xenograft mouse model, downregulation considerably inhibited solid tumors after medium (PAM) treatment. These findings underscore critical regulating apoptosis stress, highlighting promising target for NTP-based treatment HCC.

Language: Английский

Citations

1
FBXO22 promotes osteosarcoma progression via regulation of FOXO1 for ubiquitination and degradation DOI Creative Commons
He Zhang,

Yang Bai,

Jiatong Li

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(16)

Published: Aug. 1, 2024

Accumulating evidence has demonstrated that F-box protein 22 (FBXO22) participates in tumour development and progression various types of human malignancies. However, the functions detailed molecular mechanisms FBXO22 osteosarcoma tumorigenesis remain elusive. In this study, we aimed to determine effects on cell proliferation, migration invasion cells using counting kit-8 Matrigel Transwell approaches. Moreover, explored by which mediated oncogenesis via Western blotting, immunoprecipitation ubiquitination. We found depletion inhibited cells, whereas overexpression increased proliferation motility cells. Mechanistically, promoted ubiquitination degradation FoxO1 reduced regulation FoxO1. Taken together, our findings provide new insight into FBXO22-induced tumorigenesis. The inhibition could be a promising strategy for treatment osteosarcoma.

Language: Английский

Citations

1
CMTM5 influences Hippo/YAP axis to promote ferroptosis in glioma through regulating WWP2‐mediated LATS2 ubiquitination DOI Creative Commons

Fan Ye,

H. Zou

The Kaohsiung Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 40(10), P. 890 - 902

Published: Aug. 21, 2024

Abstract Glioma, a common malignancy, is characterized by high morbidity and mortality. Promoting ferroptosis can delay tumor progression. Here, we aimed to explore the underlying mechanism of in glioma. In vitro vivo experiments were conducted using glioma cells nude mice. The expression genes proteins was evaluated RT‐qPCR, Western blot assay, immunohistochemical staining. Malignant activities MTT, EdU, Transwell assays. levels Fe 2+ , lipid reactive oxygen species, malondialdehyde determined commercial kits. interplays among CMTM5, WWP2, LATS2 validated Co‐immunoprecipitation assay. UALCAN database predicted downregulation CMTM5 glioma, low associated with poor survival outcomes. overexpression inhibited cell growth invasion promoted cells. Besides, protein interacted WWP2 decreased expression. silencing attenuated ubiquitination enhance phosphorylation YAP1. exerted suppressive effect on regulating WWP2/LATS2 pathway. experiments, suppressed enhanced ferroptosis. regulated Hippo/YAP signaling inhibit promote WWP2‐mediated ubiquitination, thereby attenuating

Language: Английский

Citations

1
Role of UFMylation in tumorigenesis and cancer immunotherapy DOI Creative Commons
Lijuan Ding, Xin Jiang, Te Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 23, 2024

Protein post-translational modifications (PTMs) represent a crucial aspect of cellular regulation, occurring after protein synthesis from mRNA. These modifications, which include phosphorylation, ubiquitination, acetylation, methylation, glycosylation, Sumoylation, and palmitoylation, play pivotal roles in modulating function. PTMs influence localization, stability, interactions, thereby orchestrating variety processes response to internal external stimuli. Dysregulation is linked spectrum diseases, such as cancer, inflammatory neurodegenerative disorders. UFMylation, type PTMs, has recently gained prominence for its regulatory role numerous processes, including stress, key signaling pathways influencing functions. This review highlights the function UFMylation development progression tumors, underscoring potential therapeutic target. Moreover, we discuss tumorigenesis malignant progression, explore impact on cancer immunotherapy. The article aims provide comprehensive overview biological functions propose how targeting could enhance effectiveness immunotherapy strategies.

Language: Английский

Citations

1
Ubiquitination-related biomarkers in metastatic melanoma patients and their roles in tumor microenvironment DOI Creative Commons
Zhang Li, Zhehao Shi, Fan Zhang

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: May 19, 2023

Skin cutaneous melanoma (SKCM) is the deadliest type of malignancy. Ubiquitination a process protein sorting and degradation that exhibits multiple functions in progression various tumors. This study aimed to characterize set genes for ubiquitination SKCM.The expression patterns ubiquitin-associated (URGs) corresponding clinical information SKCM tissues were comprehensively analyzed based on The Cancer Genome Atlas (TCGA) database. We performed univariate multivariate Cox proportional regression models risk scores identify four critical related prognostic (HCLS1, CORO1A, NCF1 CCRL2), which used construct signatures. also studied effects HCLS1, CORO1A CCRL2 tumor metastasis-related indicators at cellular level through vitro experiments.SKCM patients low-risk group showing longer survival than those high-risk group. Characteristic correlated with several clinicopathological variables reflected infiltration immune cells. In addition, knockdown CLS1, affected malignant biological behavior EMT signaling pathway.This provides novel prospective strategy improve patients.

Language: Английский

Citations

3