Life Sciences, Journal Year: 2024, Volume and Issue: 358, P. 123150 - 123150
Published: Oct. 30, 2024
Language: Английский
Cancers, Journal Year: 2023, Volume and Issue: 15(15), P. 3804 - 3804
Published: July 26, 2023
Glioblastoma (GBM) is a highly aggressive and lethal primary brain cancer that necessitates early detection accurate diagnosis for effective treatment improved patient outcomes. Traditional diagnostic methods, such as imaging techniques tissue biopsies, have limitations in providing real-time information distinguishing treatment-related changes from tumor progression. Liquid used to analyze biomarkers body fluids, offer non-invasive dynamic approach detecting monitoring GBM. This article provides an overview of GBM including circulating cells (CTCs), cell-free DNA (cfDNA), RNA (cfRNA), microRNA (miRNA), extracellular vesicles. It explores the clinical utility these detection, monitoring, prognosis. Challenges implementing liquid biopsy strategies practice are also discussed. The highlights potential biopsies valuable tools personalized management but underscores need standardized protocols further research optimize their utility.
Language: Английский
Citations
21
Nanoscale, Journal Year: 2023, Volume and Issue: 15(26), P. 10904 - 10938
Published: Jan. 1, 2023
This review summarizes recent advances in extracellular vesicle-based carriers for glioblastoma diagnosis and therapy, points out the current challenges perspectives.
Language: Английский
Citations
18
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 11054 - 11054
Published: July 4, 2023
Alzheimer’s disease (AD) and Parkinson’s (PD) are the most common neurodegenerative diseases (NDDs) threatening lives of millions people worldwide, including especially elderly people. Currently, due to lack a timely diagnosis proper intervention strategy, AD PD largely remain incurable. Innovative therapy highly desired. Exosomes small vesicles that present in various bodily fluids, which contain proteins, nucleic acids, active biomolecules, play crucial role intercellular communication. In recent years, exosomes pathogenesis, early diagnosis, treatment has attracted ascending attention. However, exact pathogenesis theragnostic not been fully illustrated. review, we first introduce biogenesis, components, uptake, function exosomes. Then elaborate on involvement PD. Moreover, application therapeutics is also summarized discussed. Additionally, serving as drug carriers deliver medications central nervous system specifically addressed. The potential explored, discussing their applications treatment, well current limitations. Given limitation exosomes, propose future perspectives for better utilizing NDDs. Hopefully, it would pave ways expanding biological fundamental research theranostics
Language: Английский
Citations
16
Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(7), P. 1902 - 1902
Published: July 7, 2023
Extracellular vesicles (EVs) are small, membrane-based released by cells that play a critical role in various physiological and pathological processes. They act as vehicles for transporting variety of endogenous cargo molecules, enabling intercellular communication. Due to their natural properties, EVs have emerged promising “cell-free therapy” strategy treating diseases, including cancer. serve excellent carriers different therapeutics, nucleic acids, proteins, small other nanomaterials. Modifying or engineering can improve the efficacy, targeting, specificity, biocompatibility EV-based therapeutics cancer therapy. In this review, we comprehensively outline biogenesis, isolation, methodologies EVs, well biological functions. We then focus on specific applications drug therapy citing prominent recent studies. Additionally, discuss opportunities challenges using pharmaceutical delivery vehicles. Ultimately, aim provide theoretical technical support development treatment.
Language: Английский
Citations
11
Medicina, Journal Year: 2025, Volume and Issue: 61(4), P. 716 - 716
Published: April 13, 2025
Glioblastoma (GBM) is the most prevalent and aggressive primary central nervous system (CNS) tumor in adults. GBMs exhibit genetic epigenetic heterogeneity, posing difficulties surveillance being associated with high rates of recurrence mortality. Nevertheless, due to infiltrating ability glioblastoma cells, regardless considerable progress made radiotherapeutic, chemotherapeutic, surgical protocols, treatment GBM still inefficient. Conventional diagnostic approaches, such as neuroimaging techniques tissue biopsies, which are invasive maneuvers, present certain challenges limitations providing real-time information, incapable differentiating pseudo-progression related from real progression. Liquid biopsy, analysis biomarkers nucleic acids (DNA/RNA), circulating cells (CTCs), extracellular vesicles (EVs), or tumor-educated platelets (TEPs) that body fluids, provides a minimally dynamic method diagnosis continuous monitoring for GBM. It represents new preferred approach enables superior manner obtain data on possible risk, prognosis, assessment. This article literature review aims provide updated information about fluids analyze their clinical efficiency.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14960 - 14960
Published: Oct. 6, 2023
The mTOR signaling pathway plays a pivotal and intricate role in the pathogenesis of glioblastoma, driving tumorigenesis proliferation. Mutations or deletions PTEN gene constitutively activate by expressing growth factors EGF PDGF, which their respective receptor pathways (e.g., EGFR PDGFR). convergence pathways, such as PI3K-AKT pathway, intensifies effect activity. inhibition has potential to disrupt diverse oncogenic processes improve patient outcomes. However, complexity signaling, off-target effects, cytotoxicity, suboptimal pharmacokinetics, drug resistance inhibitors pose ongoing challenges effectively targeting glioblastoma. Identifying innovative treatment strategies address these is vital for advancing field glioblastoma therapeutics. This review discusses targets target-specific inhibitor development, optimized delivery system, implementation personalized approaches mitigate complications inhibitors. exploration precise mTOR-targeted therapies ultimately offers elevated therapeutic outcomes development more effective combat deadliest form adult brain cancer transform landscape therapy.
Language: Английский
Citations
8
iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 108022 - 108022
Published: Oct. 1, 2023
CircRNAs play multiple roles in a variety of cellular processes. We found that Circ-CDYL is highly enriched early HCC plasma exosomes. Moreover, EpCAM+ cells and exosomes had significant levels. postulated Circ-CDYL-enriched EpCAM-positive would function as liver tumor-initiating (LTi-Exos). As predicted, intercellular transfer LTi-Exos activates the HDGF-PI3K-AKT-mTOR HIF1AN-NOTCH2 axes recipient cells, promoting malignancy. Upstream, we N6-methyladenosine (m6A) modification exerted its action through dual mechanism. First, it stimulated back-splicing processes via YTHDC1 to promote biogenesis. Second, facilitates active sorting into hnRNPA2/B1. Clinically, combination alpha-fetoprotein (AFP) provides promising diagnostic biomarker for with an AUC 0.896. This study highlights effect mechanism by which m6A promotes hepatocarcinogenesis modulation tumor microenvironment LTi-Exos.
Language: Английский
Citations
8
Deleted Journal, Journal Year: 2024, Volume and Issue: 32(3), P. 200840 - 200840
Published: June 24, 2024
Brain tumors have a poor prognosis. Early, accurate diagnosis and treatment are crucial. Although brain surgical biopsy can provide an diagnosis, it is highly invasive risky not suitable for follow-up examination. Blood-based liquid biopsies low detection rate of tumor biomarkers limited evaluation ability due to the existence blood-brain barrier (BBB). The BBB composed capillary endothelial cells through tight junctions, which prevents release markers human peripheral circulation, making more difficult diagnose, predict prognosis, evaluate therapeutic response than other tumors. Focused ultrasound (FUS)-enabled (sonobiopsy) emerging technique using FUS promote into circulatory system cerebrospinal fluid, thus facilitating detection. feasibility safety data from both animal models clinical trials support sonobiopsy as great potential in diseases.
Language: Английский
Citations
2
Journal of Extracellular Biology, Journal Year: 2024, Volume and Issue: 3(8)
Published: Aug. 1, 2024
Abstract Circulating cell‐free nucleic acids are considered a promising source of biomarkers for diseases and cancer. Liquid biopsy brain tumours represent major, still unmet, clinical need. In plasma, can be free or associated with extracellular vesicles (EVs). Here we report an easy reproducible method to analyse in plasma EVs by conventional flow cytometry translate into the clinics. Nucleic present samples stained Pyronin Y, which is fluorescent dye that preferably binding double‐stranded acids. Fluorescent staining isolated from cell‐conditioned media suitable DNA RNA detection cytometry. The partially protected degradation EVs’ membrane. Additionally, plasma‐derived EVs. Remarkably, analysis patients healthy individuals reveals difference their acid profiles. Taken together, our results indicate proposed methodology, based on direct cytometry, tool analysis.
Language: Английский
Citations
2
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 14, 2023
Exosomes are progressively being detected as an indicator for the diagnosis and prognosis of cancer in clinical settings. Many trials have confirmed impact exosomes on tumor growth, particularly anti-tumor immunity immunosuppression exosomes. Therefore, we developed a risk score based genes found glioblastoma-derived In this study, used TCGA dataset training queue GSE13041, GSE43378, GSE4412, CGGA datasets external validation queue. Based machine algorithms bioinformatics methods, exosome-generalized was established. We that could independently predict patients with glioma, there were significant differences outcomes high- low-risk groups. Univariate multivariate analyses showed is valid predictive biomarker gliomas. Two immunotherapy datasets, IMvigor210 GSE78220, obtained from previous studies. A high-risk association multiple immunomodulators act immune evasion. The exosome-related effectiveness anti-PD-1 immunotherapy. Moreover, compared sensitivity scores to various anti-cancer drugs had better responses variety drugs. risk-scoring model established study provides useful tool total survival time glioma guide
Language: Английский
Citations
5