Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: 106-107, P. 103 - 105
Published: Sept. 6, 2024
Language: Английский
ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 24, 2025
The year 2024 marks the 50th anniversary of discovery surface-enhanced Raman spectroscopy (SERS). Over recent years, SERS has experienced rapid development and became a critical tool in biomedicine with its unparalleled sensitivity molecular specificity. This review summarizes advancements challenges substrates, nanotags, instrumentation, spectral analysis for biomedical applications. We highlight key developments colloidal solid an emphasis on surface chemistry, hotspot design, 3D hydrogel plasmonic architectures. Additionally, we introduce innovations including those interior gaps, orthogonal reporters, near-infrared-II-responsive properties, along biomimetic coatings. Emerging technologies such as optical tweezers, nanopores, wearable sensors have expanded capabilities single-cell single-molecule analysis. Advances analysis, signal digitalization, denoising, deep learning algorithms, improved quantification complex biological data. Finally, this discusses applications nucleic acid detection, protein characterization, metabolite monitoring, vivo spectroscopy, emphasizing potential liquid biopsy, metabolic phenotyping, extracellular vesicle diagnostics. concludes perspective clinical translation SERS, addressing commercialization potentials tissue sensing imaging.
Language: Английский
Citations
3
Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 411 - 411
Published: March 11, 2025
Cancer is one of the leading causes death worldwide. Evidence indicates that extracellular vesicles are involved in cancer development and may be used as promising biomarkers detection. Concomitantly, saliva constitutes a non-invasive inexpensive source biomarkers. This systematic review investigates use salivary detecting cancers located outside head neck. PubMed, Web Science, Scopus, Embase were thoroughly searched from database inception to 16 July 2024. Data sixteen eligible studies analyzed, including glioblastoma, lung, esophageal, gastric, prostate, hepatocellular, breast, pancreatobiliary tract cancers. The findings highlight strong diagnostic potential for lung esophageal cancers, where specific exosomal RNAs proteins demonstrated high accuracy distinguishing patients healthy individuals. Additionally, glioblastoma showed prognostic value, while those hepatocellular exhibited early However, gastric prostate limited reliability, breast require further validation. In conclusion, present detection across multiple types; however, their power needs research, standardization large-scale
Language: Английский
Citations
0
Electrophoresis, Journal Year: 2025, Volume and Issue: unknown
Published: April 6, 2025
ABSTRACT Extracellular vesicles (EVs) are pivotal in numerous physiological and pathological processes, such as immune responses, viral pathogenesis, pregnancy, cardiovascular diseases, cancer progression. Their capacity to influence complex intracellular pathways highlights their therapeutic potential addressing various conditions, including neurodegenerative diseases cancer. A novel capillary isotachophoresis (cITP) method was developed for the electrokinetic characterization of pre‐isolated EVs. Distinct peaks could be resolved at near‐baseline resolution using a mixture spacer ions laser‐induced fluorescence (LIF) detection. The were effectively separated from unbound carboxyfluorescein diacetate succinimidyl ester (CFDA‐SE) amine‐reactive fluorescent stain used detect them residual contaminants. identity shown electropherograms validated via methods, incubation with specific antibodies or spiking putative contaminants, proteins lipoproteins. This report thus provides detailed proof‐of‐concept cITP‐LIF extracellular vesicle isolation, subtype fractionation, profiling.
Language: Английский
Citations
0
Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: 106-107, P. 103 - 105
Published: Sept. 6, 2024
Language: Английский
Citations
0