Apolipoprotein-L Functions in Membrane Remodeling DOI Creative Commons
Etienne Pays

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2115 - 2115

Published: Dec. 20, 2024

The mammalian Apolipoprotein-L families (APOLs) contain several isoforms of membrane-interacting proteins, some which are involved in the control membrane dynamics (traffic, fission and fusion). Specifically, human APOL1 APOL3 appear to remodeling linked pathogen infection. Through its association with Non-Muscular Myosin-2A (NM2A), controls Golgi-derived trafficking vesicles carrying lipid scramblase Autophagy-9A (ATG9A). These deliver together phosphatidylinositol-4-kinase-B (PI4KB) activated Stimulator Interferon Genes (STING) mitochondrion-endoplasmic reticulum (ER) contact sites (MERCSs) for induction completion mitophagy apoptosis. direct interactions PI4KB activity controllers (Neuronal Calcium Sensor-1, or NCS1, Calneuron-1, CALN1, ADP-Ribosylation Factor-1, ARF1), PI(4)P synthesis. is required different processes infection-induced inflammation: (i) STING activation at Golgi subsequent lysosomal degradation inflammation termination; (ii) mitochondrion MERCSs apoptosis; (iii) phagolysosome formation antigen processing. In addition, governs mitophagosome fusion endolysosomes completion, APOL3-like murine APOL7C phagosome permeabilization cross-presentation dendritic cells. Similarly, can induce intracellular bacterial membranes, a role also be proposed endothelial APOLd1 adipocyte mAPOL6, promote angiogenesis adipogenesis, respectively, under inflammatory conditions. Thus, APOL play distinct roles associated inflammation.

Language: Английский

The Potential of Mitochondrial Therapeutics in the Treatment of Oxidative Stress and Inflammation in Aging DOI
Jitendra Kumar Sinha,

Khanak Jorwal,

Krishna Kumar Singh

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

Language: Английский

Citations

7
Single-cell mitophagy patterns within the tumor microenvironment modulate intercellular communication, impacting the progression and prognosis of hepatocellular carcinoma DOI Creative Commons

Zhengyan Li,

Wei Chen, Yao Shu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

Hepatocellular carcinoma (HCC) is a common malignant tumor of the digestive system with high incidence that seriously threatens patients' lives and health. However, rise application new treatments, such as immunotherapy, there are still some restrictions in treatment diagnosis HCC, therapeutic effects on patients not ideal. Two single-cell RNA sequencing (scRNA-seq) datasets from HCC patients, encompassing 25,189 cells, were analyzed study. We utilized non-negative matrix factorization (NMF) clustering to identify mitophagy patterns TME including cancer-associated fibroblasts (CAFs), T B tumor-associated macrophages (TAMs). Cell-to-cell communication was using CellChat package, pseudotime trajectory analysis performed Monocle package. Gene regulatory networks investigated SCENIC survival analyses conducted mitophagy-related signatures. samples identified 22 clusters, 7 principal cell types. Complex communications observed among these Mitophagy-related CAFs, TAMs, CD8+ cells identified. These subtypes had different biological states, cell-cell communications, metabolic pathways. Mitophagy levels elevated samples. Changes genes within specific associated overall rates. did seem affect effectiveness immunotherapy. This study provides evidence modulates intercellular communication, influencing progression patient prognosis. Targeting may offer promising approach improve long-term prognosis patients.

Language: Английский

Citations

0
Mitochondria-Targeted Antioxidant (MitoQ) and Nontargeted Antioxidant (Idebenone) Mitigate Mitochondrial Dysfunction in Corneal Endothelial Cells DOI
Myriam Böhm, Mohit Parekh, Neha Deshpande

et al.

Cornea, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Purpose: To investigate the effectiveness of mitochondrial-targeted antioxidant mitoquinone (MitoQ) and nontargeted idebenone (Idb) in alleviating mitochondrial dysfunction corneal endothelial cells (CEnCs). Methods: In vitro experiments were conducted using immortalized normal human (HCEnC-21T; SVN1-67F) Fuchs dystrophy (FECD) (SVF5-54F; SVF3-76M). Cells pretreated with MitoQ or Idb then exposed to menadione (MN) simultaneous treatment. Mitochondrial parameters evaluated through adenosine triphosphate viability assays, JC-1 staining for membrane potential, Tom-20 antibody fragmentation, analysis performed ImageJ software. HCEnC-21T additionally ultraviolet-A (25 J/cm 2 ) assess drug effects under physiological stress. fragmentation FECD specimens was analyzed pre- post-treatment drugs. Statistical 1-/2-way variance post-hoc Tukey test. Results: enhanced cell potential both MN-induced reduced by 32% more than 13% SVF5-54F cells. Under stress, improved function 31% 25%, respectively, increasing 42% specimens. Conclusions: Differential responses across lines highlight disease heterogeneity. protected CEnCs from oxidative stress bioenergetics, suggesting that antioxidants could be considered CEnCs.

Language: Английский

Citations

0
Molelcuar regulation of mitophagy signaling in tumor microenvironment and its targeting for cancer therapy DOI
Bishnu Prasad Behera, Soumya Ranjan Mishra, Srimanta Patra

et al.

Cytokine & Growth Factor Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Circadian clockwork controls the balance between mitochondrial turnover and dynamics: What is life … without time marking? DOI

Olga Cela,

Rosella Scrima,

Michela Rosiello

et al.

Biochimica et Biophysica Acta (BBA) - Bioenergetics, Journal Year: 2025, Volume and Issue: unknown, P. 149542 - 149542

Published: Jan. 1, 2025

Language: Английский

Citations

0
Omega-3 PUFAs improve cognitive function in heat-stressed mice by enhancing autophagy via inhibition of the phosphorylation of the PI3K-Akt-mTOR pathway DOI

Zifu Ren,

Mengyu Cai,

Xinyao Liu

et al.

Food & Function, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The adverse effects of elevated temperatures on human health are becoming progressively severe.

Language: Английский

Citations

0
A critical role for Phocaeicola vulgatus in negatively impacting metformin response in diabetes DOI Creative Commons
Man‐Yun Chen,

Yilei Peng,

Yuhui Hu

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Galectin-8 drives ERK-dependent mitochondrial fragmentation, perinuclear relocation and mitophagy, with metabolic adaptations for cell proliferation DOI Creative Commons
Adely de la Peña, Cláudio Retamal,

Francisca Pérez-Molina

et al.

European Journal of Cell Biology, Journal Year: 2025, Volume and Issue: unknown, P. 151488 - 151488

Published: April 1, 2025

Mitochondria adapt to the cell proliferative demands induced by growth factors through dynamic changes in morphology, distribution, and metabolic activity. Galectin-8 (Gal-8), a carbohydrate-binding protein that promotes proliferation transactivating EGFR-ERK signaling pathway, is overexpressed several cancers. However, its impact on mitochondrial dynamics during remains unknown. Using MDCK RPTEC kidney epithelial cells, we demonstrate Gal-8 induces fragmentation perinuclear redistribution. Additionally, mitochondria adopt donut-shaped morphologies, live-cell imaging with two Keima-based reporters demonstrates Gal-8-induced mitophagy. ERK inhibition abrogates all these proliferation. Studies established mutant versions of CHO cells reveal response require interactions between N-terminal carbohydrate recognition domain α-2,3-sialylated N-glycans at surface. DRP1, key regulator fission, becomes phosphorylated or an ERK-dependent manner, mediating Bafilomycin A proliferation, suggesting mitophagy serves as adaptation demands. Functional analysis under stimulation shows maintain active electron transport chain, partially uncoupled from ATP synthesis, increased membrane potential, indicative healthy mitochondria. Meanwhile, exhibit extracellular acidification rate lactate production via aerobic glycolysis, hallmark state. Our findings integrate adaptations potential implications for physiology, disease, therapeutic strategies.

Language: Английский

Citations

0
DRP1, fission and apoptosis DOI Creative Commons
Nan Wang,

X.J. Wang,

Beiwu Lan

et al.

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 7, 2025

Language: Английский

Citations

0
Natural small molecules regulating the mitophagy pathway counteract the pathogenesis of diabetes and chronic complications DOI Creative Commons
Ye Du,

Junping Zhu,

Shengrui Su

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Diabetes mellitus (DM) is a chronic metabolic disorder marked by sustained hyperglycemia. These disturbances contribute to extensive damage across various tissues and organs, giving rise severe complications such as vision loss, kidney failure, amputations, higher morbidity mortality rates. Furthermore, DM imposes substantial economic emotional burden on patients, families, healthcare systems. Mitophagy, selective process that targets the clearance of damaged or dysfunctional mitochondria, pivotal for sustaining cellular homeostasis through mitochondrial turnover recycling. Emerging evidence indicates mitophagy acts key pathogenic driver in pathogenesis its associated complications. Natural small molecules are particularly attractive this regard, offering advantages low toxicity, favorable pharmacokinetic profiles, excellent biocompatibility, broad range biochemical activities. This review systematically evaluates mechanistic roles natural molecules-including ginsenosides, resveratrol, berberine-in enhancing restoring via activation core signaling pathways (e.g., PINK1/Parkin, BNIP3/NIX, FUNDC1). collectively ameliorate pathological hallmarks DM, oxidative stress, inflammation, insulin resistance. integration nanotechnology with these compounds optimizes their bioavailability tissue-specific targeting, thereby establishing transformative therapeutic platform management. Current demonstrates modulation not only offers novel strategies but also advances foundation future drug development targeting disorders.

Language: Английский

Citations

0