Genome sequencing and analysis of Bacillus velezensis VJH504 reveal biocontrol mechanism against cucumber Fusarium wilt DOI Creative Commons

Fan Yang,

Huayan Jiang,

Kai Ma

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 12, 2023

One major issue in reducing cucumber yield is the destructive disease Cucumber ( Cucumis sativus L.) wilt caused by Fusarium oxysporum f. sp. cucumerinum Foc ). When using isolate VJH504 isolated from rhizosphere soil and identified as Bacillus velezensis , growth of double culture experiment was effectively inhibited. Phenotypic, phylogenetic, genomic analyses were conducted to enhance understanding its biocontrol mechanism. According result phenotype analysis, B. could inhibit fusarium both vitro vivo significantly promote seed germination seedling growth. Additionally, tests growth-promoting characteristics revealed secretion proteases, amylases, β-1,3-glucanases, cellulases, well siderophores indole-3-acetic acid VJH504. Using PacBio Sequel II system, we applied complete genome sequencing for obtained a single circular chromosome with size 3.79 Mb. A phylogenetic tree constructed based on 16S rRNA gene sequences 13 other species, Average Nucleotide Identity (ANI) analysis performed their whole-genome sequences, confirming isolateVJH504 . Following this, sequence od VJH504, specific functional Carbohydrate-Active Enzymes (CAZymes) secondary metabolite carried out, predicting organism’s abilities biofilm formation, production antifungal CAZymes, synthesis antagonistic metabolites against pathogens. Afterwards, comparative between three strains, revealing subtle differences suggesting potential discovery novel antimicrobial substances In conclusion, mechanism controlling predicted appear that VJH504is promising agent, showcasing excellent application agricultural production.

Language: Английский

A Review of the Most Common and Economically Important Diseases That Undermine the Cultivation of Tomato Crop in the Mediterranean Basin DOI Creative Commons
Stefano Panno, Salvatore Davino, A. Caruso

et al.

Agronomy, Journal Year: 2021, Volume and Issue: 11(11), P. 2188 - 2188

Published: Oct. 29, 2021

Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years one of most important and extensively grown horticultural crops Mediterranean region throughout world. In 2019, more than 180 million tonnes tomato have been produced worldwide, out which around 42 countries. Due to its genetic properties, is afflicted by numerous plant diseases induced fungal, bacterial, phytoplasma, virus, viroid pathogens. Not only inheritance great importance management pathogens, but equally as are also present climate changes, recently revised phytopathological control measures, globalization seed industry. Thus, recognition symptoms knowledge distribution spread disease methods for early detection pathogens major prerequisites a successful disease. this review, we will describe main area that impact mostly yield provide current perspective measures necessary their management.

Language: Английский

Citations

212
Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease DOI Creative Commons
Xin Zhou, Jinting Wang, Fang Liu

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 22, 2022

Abstract The role of rhizosphere microbiota in the resistance tomato plant against soil-borne Fusarium wilt disease (FWD) remains unclear. Here, we showed that FWD incidence was significantly negatively correlated with diversity both bacterial and fungal communities. Using microbiological culturomic approach, selected 205 unique strains to construct different synthetic communities (SynComs), which were inoculated into germ-free seedlings, their roles suppressing monitored using omics approach. Cross-kingdom (fungi bacteria) SynComs most effective than those Fungal or Bacterial alone. This effect underpinned by a combination molecular mechanisms related immunity microbial interactions contributed study provides new insight dynamics pathogen suppression host interactions. Also, formulation manipulation for functional complementation constitute beneficial strategy controlling disease.

Language: Английский

Citations

120
Major Soilborne Pathogens of Field Processing Tomatoes and Management Strategies DOI Creative Commons

Minxiao Ma,

P. W. J. Taylor, Deli Chen

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(2), P. 263 - 263

Published: Jan. 19, 2023

Globally, tomato is the second most cultivated vegetable crop next to potato, preferentially grown in temperate climates. Processing tomatoes are generally produced field conditions, which soilborne pathogens have serious impacts on yield and quality by causing diseases of root system. Major processing tomato-producing countries documented caused a variety including bacteria, fungi, nematodes, oomycetes, economic importance may threaten food security. Recent surveys Australian industry showed that plant growth were significantly affected pathogens, especially Fusarium oxysporum Pythium species. different management methods been used control such as use resistant cultivars, application fungicides, biological control. Among these methods, biocontrol has received increasing attention due its high efficiency, target-specificity, sustainability public acceptance. The mix strategies, applying antagonistic microorganisms field, using beneficial metabolites synthesized microorganisms. This review provides broad major fungal/oomycete affecting global producers, traditional practices for various strategies diseases. advantages disadvantages discussed, highlighted managing under pressure climate change.

Language: Английский

Citations

48
Bacillus Species: Excellent Biocontrol Agents against Tomato Diseases DOI Creative Commons
Vasiljka Karačić,

Dragana Miljaković,

Jelena Marinković

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 457 - 457

Published: Feb. 24, 2024

Tomatoes encounter many pathogens, such as fungi and bacteria, which reduce the yield quality of plants lead to large losses in production. The application plant protection products (PPPs) is still an important most effective measure control diseases. However, use chemicals agriculture contributes environmental pollution biodiversity loss, it can also threaten non-target living organisms. Biological a widely accessible, environmentally safe, cost-efficient alternative for prevention suppression

Language: Английский

Citations

30
Biofertilizer and biocontrol properties of Stenotrophomonas maltophilia BCM emphasize its potential application for sustainable agriculture DOI Creative Commons
Pinki Sharma, Rajesh Pandey, Nar Singh Chauhan

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 4, 2024

Introduction Microbial biofertilizers or biocontrol agents are potential sustainable approaches to overcome the limitations of conventional agricultural practice. However, limited catalog microbial candidates for diversified crops creates hurdles in successfully implementing agriculture increasing global/local populations. The present study aimed explore wheat rhizosphere microbiota strains with a biofertilizer and potential. Methods Using culturing-based approach, 12 unique isolates were identified screened biofertilizer/biocontrol using genomics physiological experimentations. Results discussion Molecular, physiological, phylogenetic characterization Stenotrophomonas maltophilia BCM as candidate agriculture. was coccus-shaped gram-negative microbe having optimal growth at 37°C partially alkaline environment (pH 8.0) proliferation time ~67 minutes. stress response physiology indicates its successful survival dynamic environmental conditions. It significantly increased (P <0.05) seed germination percentage presence phytopathogens saline Genomic decoded genes involved plant promotion, nutrient assimilation, antimicrobial activity. Experimental evidence also correlates genomic insights explain agent. With these properties, could sustainably promote production ensure food security population, especially native wheat-consuming areas.

Language: Английский

Citations

11
Use of biological control agents for managing fungal pathogens in Solanaceae crops: progress and future perspectives—a review DOI Creative Commons

Sinhle Madlhophe,

Udoka Vitus Ogugua, Fikile Nelly Makhubu

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 15, 2025

The growing global population has intensified concerns about food security, making it essential to produce crops sustainably meet increasing demands without harming the environment. In this regard, biological control agents (BCAs) have recently gained more attention owing their potential manage fungal diseases of crops, particularly in Solanaceae family. proper use selected BCAs such as Trichoderma spp., Bacillus Pseudomonas fluorescens, Beauveria bassiana, and Gliocladium spp. several benefits for crops. This review aims summarize effectiveness various strategies We also provide basic knowledge on along with suggestions further research reduce severity these destructive diseases.

Language: Английский

Citations

1
Employing algal extract for the fabrication of bimetallic nanoparticles: A powerful solution against tomato pathogenic fungi DOI Creative Commons
Reham M. Aldahasi, Kawther Aabed, Sahar S. Alghamdi

et al.

AIP Advances, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 1, 2025

This study explores the innovative use of an aqueous spirulina and chlorella mixture to synthesize bimetallic zinc/manganese nanoparticles (ZnO–Mn NPs), showcasing a sustainable approach nanomaterial development. The were thoroughly characterized using advanced techniques, including UV–visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering, scanning electron microscopy, transmission energy-dispersive x-ray spectroscopy. These analyses revealed that ZnO–Mn NPs have average size 314.4 nm, polydispersity index 0.28, zeta potential −23.86 mV, indicating excellent stability uniform distribution. antifungal properties synthesized tested in vitro against key tomato pathogens, Sclerotinia sclerotiorum Fusarium equiseti. FTIR analysis highlighted role phytochemicals from algal extracts reduction capping nanoparticles. While activity observed was modest at evaluated concentrations, strong biocompatibility underscore their as eco-friendly solutions agricultural applications. research highlights promise utilizing natural develop effective nanoparticles, offering greener alternative for managing plant diseases enhancing farming practices.

Language: Английский

Citations

1
Changes in Bacterial and Fungal Microbiomes Associated with Tomatoes of Healthy and Infected by Fusarium oxysporum f. sp. lycopersici DOI
Xin Zhou, Jinting Wang, Weihua Wang

et al.

Microbial Ecology, Journal Year: 2020, Volume and Issue: 81(4), P. 1004 - 1017

Published: June 25, 2020

Language: Английский

Citations

58
Exploring the efficacy of antagonistic rhizobacteria as native biocontrol agents against tomato plant diseases DOI

S. Karthika,

Sherin Varghese,

M. S. Jisha

et al.

3 Biotech, Journal Year: 2020, Volume and Issue: 10(7)

Published: June 27, 2020

Language: Английский

Citations

50
Biostimulation of tomato growth and biocontrol of Fusarium wilt disease using certain endophytic fungi DOI Creative Commons
Amer M. Abdelaziz, Mohamed H. Kalaba, Amr H. Hashem

et al.

Botanical studies, Journal Year: 2022, Volume and Issue: 63(1)

Published: Dec. 9, 2022

Tomato plant (Solanum lycopersicum L.) suffers from numerous fungal pathogens that cause damage to yeild production qualitatively and quantitatively. One of the most destructive disease tomato is Fusarium wilt caused by soil borne fungus called F. oxysporum.In this study, anti-Fusarium capabilities foliar application endophytes extracts have been investigated on under challenges. Antifungal assay, inhibition conidial germination, severity, photosynthetic pigments, osmolytes, secondary metabolites, oxidative stress, peroxidase (POD) polyphenol oxidases (PPO) isozymes were tested for potential resistance growing infection.Ethyl acetate A. flavus MZ045563, fumigatus MZ045562 nidulans MZ045561 exhibited antifungal activity toward oxysporum where zone diameters 15, 12 20 mm, respectively. Moreover, all isolates at concentration 7.5 mg/mL reduced conidia germination 94.4 100%. infection a effects plant, high severity desiese index 84.37%, reduction in growth parameters, soluble protein. However, contents proline, total phenol, malondialdehyde (MDA), hydrogen peroxide (H2O2) antioxidant enzymes increased plants grown wilt. Treatment healthy or infected ethyl showed improvements morphological traits, phenol activity. Besides, harmful impacts also lowering MDA H2O2 levels. Also, treated different responses number density POD PPO isozymes.It could be suggested especially combination flavus, commercially used as safe biostimulation well biofungicide against disease.

Language: Английский

Citations

38