SARS-CoV-2 neutralizing antibody determination after vaccination using spectrophotometric measurement of lateral flow immunochromatography

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

Neutralizing antibody titers have been found to be strongly correlated with observed vaccine effectiveness against symptomatic and severe COVID-19. Few non-high complexity assays are currently available detect the presence of neutralizing antibodies. This retrospective single-center cross-sectional study compared performance a lateral flow immunochromatography assay coupled spectrophotometric measurement system for detecting SARS-CoV-2 antibodies an enzyme-linked immunosorbent (ELISA) neutralization in context post-vaccination responses. The limit detection was similar ELISA strong linearity throughout measuring interval. Repeatability, interfering substances, cross-reactivity studies were robust. Results 274 plasma samples on whom RNA test vaccination status, including number manufacturer, known showed positive predictive value (PPV) 99.0% (CI 96.4–99.7%) negative (NPV) 91.9% 83.4–96.2%) ELISA. PPV all manufacturer subgroups > 95% except those individuals who had only 1 Pfizer (PPV 80%). NPV PCR no vaccinations 100% while 88.1% without previous or vaccination. 80% contrast Moderna vaccinations. Alternative methodologies requiring less sophisticated laboratory support measure may useful

Language: Английский

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Effectiveness of the first and second SARS-CoV-2 vaccine dose: a nationwide cohort study from Austria on hybrid versus natural immunity

Open Forum Infectious Diseases, Journal Year: 2024, Volume and Issue: 11(10)

Published: Sept. 17, 2024

Abstract Background We aimed to evaluate the effectiveness of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccinations in previously SARS-CoV-2–infected adults general population Austria during Delta wave and with extended follow-up. Methods In a nationwide retrospective cohort study, we calculated age-, sex-, nursing home residency–adjusted Cox proportional hazard ratios (HRs) disease 2019 (COVID-19) deaths, SARS-CoV-2 infections, non-COVID-19 deaths from 1 October 31 December 2021, secondarily follow-up 30 June 2022. Relative vaccine (rVE) is rVE = (1 – HR) × 100. Results Among 494 646 infected adults, 169 543 had received doses, 133 567 dose, 190 275 were unvaccinated at baseline. recorded 17 COVID-19 (6 vaccinated, 11 unvaccinated) 8209 infections. Absolute risk was 0.003%. estimates for reinfections exceeded 75% until end 2021 but decreased substantially The death lower those vaccinated versus unvaccinated. Conclusions First second doses appear effective short-term, diminishing over time. extremely low mortality, regardless vaccination, indicates strong protection previous infection against death. Lower mortality might suggest healthy vaccinee bias.

Language: Английский

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The world’s largest experiment in human immunology

Seminars in Immunology, Journal Year: 2024, Volume and Issue: 73, P. 101888 - 101888

Published: May 1, 2024

Language: Английский

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Coronaviruses: Pandemic SARS-CoV-2

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 79

Published: Dec. 18, 2024

Language: Английский

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