ACS Applied Nano Materials,
Journal Year:
2023,
Volume and Issue:
6(13), P. 11630 - 11639
Published: June 15, 2023
In-situ
determination
of
cell-secreted
biomolecules
is
important
for
elucidating
its
regulation
signal
transduction
pathways,
but
detecting
these
low-level
endogenous
difficult.
Herein,
using
H2O2
as
the
detection
object,
we
developed
an
ultrasensitive
enzyme-free
ratiometric
electrochemical
sensing
platform
in
a
simple
and
low-cost
method
in-situ
H2O2,
which
ferrocene
(Fc)
acts
internal
reference
probe
nanoflower
Au-Co-MOF
probe.
First,
flower-like
CoFc-MOF
was
synthesized
situ
on
flexible
carbon
cloth
(CC)
by
one-step
hydrothermal
method,
then
Au-CoFc-MOF/CC
obtained
electrodeposition
gold
nanoparticles.
The
measurement
results
show
that
presence
increases
reduction
peak
Co-MOF
(−0.32
V),
while
Fc
(+0.4
V)
almost
unchanged.
By
taking
full
advantage
obviously
separated
potential
Co-MOF,
well-designed
can
reflect
concentration
based
IH2O2
–
Iblank/IFc
value
0.5
μM–10
mM
range,
with
limit
low
32
nM,
∼15.6
times
lower
than
from
single
method.
In
addition,
also
demonstrates
excellent
selectivity,
reproducibility,
stability,
biocompatibility.
More
significantly,
this
substrate
shows
great
application
provides
feasible
strategy
other
active
substances
released
living
cells
clinical
diagnosis.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(18), P. 6930 - 6939
Published: April 23, 2024
Circulating
tumor
DNA
(ctDNA)
holds
great
promise
as
a
noninvasive
biomarker
for
cancer
diagnosis,
treatment,
and
prognosis.
However,
the
accurate
specific
quantification
of
low-abundance
ctDNA
in
serum
remains
significant
challenge.
This
study
introduced,
first
time,
novel
exponential
amplification
reaction
(EXPAR)-assisted
CRISPR/Cas12a-mediated
ratiometric
dual-signal
electrochemical
biosensor
ultrasensitive
reliable
detection
ctDNA.
To
implement
strategy,
signal
unit
(ssDNA-MB@Fc/UiO-66-NH
Microchimica Acta,
Journal Year:
2022,
Volume and Issue:
189(4)
Published: March 12, 2022
Determination
of
specific
cardiac
biomarkers
(CBs)
during
the
diagnosis
and
management
adverse
cardiovascular
events
such
as
acute
myocardial
infarction
(AMI)
has
become
commonplace
in
emergency
department
(ED),
cardiology
many
other
ward
settings.
Cardiac
troponins
(cTnT
cTnI)
natriuretic
peptides
(BNP
NT-pro-BNP)
are
preferred
clinical
practice
for
diagnostic
workup
AMI,
coronary
syndrome
(ACS)
types
ischaemia
heart
failure
(HF),
while
roles
possible
applications
several
potential
continue
to
be
evaluated
subject
comprehensive
reviews.
The
requirement
rapid,
repeated
testing
a
small
number
CBs
ED
patients
led
development
point-of-care
(PoC)
technology
circumvent
need
remote
lengthy
procedures
hospital
pathology
laboratories.
Electroanalytical
sensing
platforms
have
meet
these
requirements.
This
review
aims
firstly
reflect
on
benefits
rapid
CB
critically
ill
patients,
very
distinct
cohort
with
deranged
baseline
levels
CBs.
We
summarise
their
source
relevance
first
report
required
analytical
ranges
value
this
patient
cohort.
Secondly,
we
current
electrochemical
approaches,
including
its
sub-variants
photoelectrochemical
electrochemiluminescence,
determination
important
highlighting
various
strategies
used,
namely
use
micro-
nanomaterials,
maximise
sensitivities
selectivities
approaches.
Finally,
consider
challenges
that
must
overcome
allow
commercialisation
transition
into
intensive
care
medicine.
