Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Sensors, Journal Year: 2021, Volume and Issue: 21(15), P. 5185 - 5185

Published: July 30, 2021

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform on-site detection target substances. LFIA can be considered as a sort lab-in-a-hand and, together with other point-of-need tests, has represented paradigm shift from sample-to-lab lab-to-sample aiming improve decision making and turnaround time. features LFIAs made them very attractive tool in clinical diagnostic where they patient care enabling more prompt diagnosis treatment decisions. rapidity, simplicity, relative cost-effectiveness, possibility used nonskilled personnel contributed wide acceptance LFIAs. As consequence, molecules, organisms, (bio)markers for purposes, application been rapidly extended fields, including food feed safety, veterinary medicine, environmental control, many others. This review aims provide readers 10-years overview applications, outlining trends main fields compounded annual growth rates. Moreover, future perspectives challenges are discussed.

Language: Английский

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Smartphone-Based Electrochemical Immunoassay for Point-of-Care Detection of SARS-CoV-2 Nucleocapsid Protein

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(43), P. 15155 - 15161

Published: Oct. 17, 2022

Large-scale, rapid, and inexpensive serological diagnoses of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are great interest in reducing virus transmission at the population level; however, their development is greatly plagued by lack available point-of-care methods, leading to low detection efficiency. Herein, an ultrasensitive smartphone-based electrochemical immunoassay reported for rapid (less than 5 min), low-cost, easy-to-implement SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 N protein). Specifically, was fabricated on a screen-printed carbon electrode coated with electrodeposited gold nanoparticles, followed incubation anti-N antibody (Ab) bovine serum albumin as working electrode. Accompanied antigen-antibody reaction between Ab, electron transfer electroactive species [Fe(CN)6]3-/4- surface disturbed, resulting reduced square-wave voltammetry currents 0.075 V versus Ag/AgCl reference The proposed provided good linear range concentrations within scope 0.01-1000 ng/mL (R2 = 0.9992) limit down 2.6 pg/mL. Moreover, data wirelessly transmitted interface smartphone, corresponding concentration value calculated displayed. Therefore, portable mode offers potential self-differential diagnosis residents, which will facilitate effective control large-scale screening resource-limited areas.

Language: Английский

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The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: April 27, 2023

Nanozymes represent a class of nanosized materials that exhibit innate catalytic properties similar to biological enzymes. The unique features these have positioned them as promising candidates for applications in clinical sensing devices, specifically those employed at the point-of-care. They notably found use means amplify signals nanosensor-based platforms and thereby improve sensor detection limits. Recent developments understanding fundamental chemistries underpinning enabled development highly effective nanozymes capable clinically relevant biomarkers limits compete with "gold-standard" techniques. However, there remain considerable hurdles need be overcome before nanozyme-based sensors can utilized platform ready use. An overview current understandings disease diagnostics biosensing unmet challenges must considered prior their translation diagnostic tests is provided.

Language: Английский

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Nanozymes: Applications in clinical biomarker detection

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(4)

Published: Sept. 19, 2023

Abstract Enzymes are highly efficient catalysts employed in the biomedical field. However, their use is limited by poor stability and high cost. To address this, there an imperative need for research on nanozymes. In last decade, introduction of nanozymes has led to exponential growth nanozyme‐based biosensors. The rapid precise detection clinical biomarkers crucial various disease diagnostics. recent years, researchers have developed different catalytic active nanomaterials combined them with colorimetric, fluorescent, electrochemical methods detect biomarkers. This review critically overviews progress, future prospects, challenges biomarker assays past few years.

Language: Английский

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Recent advancements of smartphone-based sensing technology for diagnosis, food safety analysis, and environmental monitoring

Talanta, Journal Year: 2024, Volume and Issue: 275, P. 126080 - 126080

Published: April 10, 2024

Language: Английский

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A nanoenzyme linked immunochromatographic sensor for rapid and quantitative detection of SARS-CoV-2 nucleocapsid protein in human blood

Sensors and Actuators B Chemical, Journal Year: 2021, Volume and Issue: 349, P. 130718 - 130718

Published: Sept. 12, 2021

Language: Английский

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Applications of smartphone-based colorimetric biosensors

Biosensors and Bioelectronics X, Journal Year: 2022, Volume and Issue: 11, P. 100173 - 100173

Published: May 28, 2022

Language: Английский

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Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

Chemical Science, Journal Year: 2023, Volume and Issue: 14(23), P. 6149 - 6206

Published: Jan. 1, 2023

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.

Language: Английский

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Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Small Methods, Journal Year: 2023, Volume and Issue: 7(11)

Published: Oct. 10, 2023

Abstract Nanozymes show great promise in enhancing disease biomarker sensing by leveraging their physicochemical properties and enzymatic activities. These qualities facilitate signal amplification matrix effects reduction, thus boosting performance. In this review, recent studies from the last five years, concentrating on detection improvement through nanozyme‐based biosensing are examined. This enhancement primarily involves modulations of size, morphology, doping, modification, electromagnetic mechanisms, electron conduction efficiency, surface plasmon resonance nanozymes for increased sensitivity. addition, a comprehensive description synthesis tuning strategies employed has been provided. includes detailed elucidation catalytic mechanisms alignment with fundamental principles enhanced technology, accompanied presentation quantitatively analyzed results. Moreover, diverse applications strip sensing, colorimetric electrochemical surface‐enhanced Raman scattering have outlined. Additionally, limitations, challenges, corresponding recommendations concerning application summarized. Furthermore, insights offered into future development outlook biosensing. review aims to serve not only as reference sensitivity biosensors but also catalyst exploring nanozyme broader

Language: Английский

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Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses

Discover Nano, Journal Year: 2023, Volume and Issue: 18(1)

Published: April 1, 2023

Abstract Recent years have witnessed the emergence of several viruses and other pathogens. Some these infectious diseases spread globally, resulting in pandemics. Although biosensors various types been utilized for virus detection, their limited sensitivity remains an issue. Therefore, development better diagnostic tools that facilitate more efficient detection pathogens has become important. Nanotechnology recognized as a powerful tool viruses, it is expected to change landscape analysis. Recently, nanomaterials gained enormous attention value improving biosensor performance owing high surface-to-volume ratio quantum size effects. This article reviews impact nanotechnology on design, development, sensors viruses. Special paid nanoscale materials, nanobiosensors, internet medical things, artificial intelligence-based viral techniques.

Language: Английский

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