Biosensors,
Journal Year:
2023,
Volume and Issue:
13(9), P. 857 - 857
Published: Aug. 29, 2023
Point-of-care
tests
play
an
important
role
in
serological
diagnostics
of
infectious
diseases
and
post-vaccination
immunity
monitoring,
including
COVID-19.
Currently,
lateral
flow
dominate
this
area
show
good
analytical
performance.
However,
studies
to
improve
the
effectiveness
such
remain
important.
In
comparison
with
tests,
vertical
immunoassays
allow
for
a
reduction
assay
duration
influence
hook
effect.
Additionally,
use
carbon
black
nanoparticles
(CNPs)
as
color
label
can
provide
lower
detection
limit
(LOD)
compared
conventional
colloidal
gold.
Therefore,
we
have
developed
immunoassay
IgG
against
SARS-CoV-2
spike
protein
human
serum
samples
by
applying
conjugate
CNPs
anti-human
mouse
monoclonal
antibodies
(CNP@MAb).
The
device
consists
plastic
cassette
hole
on
its
top
containing
nitrocellulose
membrane
coated
absorbent
pad.
sample,
washing
buffer,
CNP@MAb
vertically
through
pads,
reducing
time
simplifying
procedure.
positive
samples,
interaction
anti-spike
leads
appearance
spots,
which
be
visually
detected.
method
allows
rapid
visual
(5–7
min)
vs.
protein,
LOD
7.81
BAU/mL.
It
has
been
shown
that
untrained
operator
perform
evaluate
results.
Thus,
presented
used
further
development
test
systems
COVID-19
or
monitoring.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(33), P. 12532 - 12540
Published: Aug. 8, 2023
Rapid
and
sensitive
antigen
detection
using
a
lateral
flow
immunoassay
(LFIA)
is
crucial
for
diagnosing
infectious
diseases
due
to
its
simplicity,
speed,
user-friendly
features.
However,
it
remains
critical
issue
explore
specific
biorecognition
elements
powerful
signal
amplification.
In
this
study,
taking
SARS-CoV-2
as
proof
of
concept,
peptide,
WFLNDSELIML,
binding
the
spike
(S)
was
identified
by
nonamplified
biopanning
method,
which
exhibited
high
affinity
target,
with
dissociation
constant
9.29
±
1.55
nM.
Molecular
docking
analysis
reveals
that
peptide
binds
N-terminal
domain
S
antigen.
Then,
capture
probe
angiotensin-converting
enzyme
2
probe,
peptide-based
(pLFIA)
without
any
antibody
developed,
polydopamine
nanosphere
(PDA)@MnO2
nanocomposite
excellent
oxidase-like
activity
used
colorimetric
label,
exhibiting
dual-mode
remarkable
amplification
natural
melanin
on-demand
nanozyme
catalytic
enhancement.
The
PDA@MnO2-based
pLFIA
capable
detecting
limit
8.01
pg/mL,
18.7
times
lower
than
conventional
tagged
gold
nanoparticles.
Additionally,
as-proposed
can
detect
up
150
transduction
units/mL
pseudoviruses
spiked
in
saliva
samples.
Given
outstanding
analytical
performance,
proposed
may
offer
reliable
option
rapid
diagnosis
SARS-CoV-2.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(8), P. 3291 - 3299
Published: Feb. 2, 2024
Lateral
flow
assay
(LFA)-based
rapid
antigen
tests
are
experiencing
extensive
global
uptake
as
an
expeditious
and
highly
effective
modality
for
the
screening
of
viral
infections
during
COVID-19
pandemic.
While
these
devices
have
played
a
significant
role
in
alleviating
burden
on
public
healthcare
system,
their
specificity
sensitivity
fall
short
compared
with
molecular
tests.
In
this
study,
we
endeavor
to
address
both
limitations
through
utilization
DNA
nanotechnology
LFA
format,
wherein
substitute
target-specific
antibody
designer
nanostructure-based
probes
recognizing
SARS-CoV-2
virus
via
multivalent,
pattern-matching
interactions.
We
meticulously
designed
Net-shaped
nanostructure
strategically
arranged
trimeric
clusters
aptamers
that
specifically
recognize
spike
proteins
SARS-CoV-2.
This
approach
has
proven
instrumental
bolstering
virus-binding
affinity
LFAs.
Our
findings
indicate
high
sensitivity,
enabling
detection
loads
ranging
from
103
108
copies/mL.
notable
is
maintained
across
various
strains,
obviating
need
intricate
sample
preparation
protocols.
The
significance
heightened
lies
crucial
by
nanostructure,
which
facilitates
extremely
low
levels
loads.
not
only
enhances
overall
reliability
self-testing
but
also
reduces
likelihood
false-negative
results,
especially
cases
load
within
patient
samples.
Chemical Science,
Journal Year:
2024,
Volume and Issue:
15(45), P. 18715 - 18750
Published: Jan. 1, 2024
The
construction
of
functional
structures
within
a
single
plane
still
has
lot
challenges.
This
review
article
will
help
us
find
new
groundbreaking
directions
in
lateral
nanoarchitectonics
toward
advanced
material
organization.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(30), P. 11359 - 11364
Published: July 19, 2023
Signal
amplification
techniques
are
highly
desirable
for
the
analysis
of
low-level
targets
that
closely
related
with
diseases
and
monitoring
important
biological
processes.
However,
it
is
still
challenging
to
achieve
this
goal
in
a
facile
economical
way.
Herein,
we
developed
novel
dual
signal
strategy
by
combining
urease
catalysis
release
Ag+
from
silver
nanoparticles
(AgNPs).
This
was
used
quantifying
DNA
sequence
(HIV-1)
human
immunodeficiency
virus
(HIV).
target
HIV-1
hybridizes
capture
probe
on
magnetic
beads
reporter
AgNPs,
forming
sandwich
complex.
The
captured
AgNPs
then
transformed
into
numerous
ions
inactivate
ureases.
Without
catalytic
production
ammonia
urea,
substrate
solution
shows
low
pH
5.8
will
increase
otherwise.
change
monitored
indicator
(phenol
red),
which
allows
colorimetric
detection.
proposed
approach
sensitive,
easy
use,
economic,
universal,
exhibiting
detection
limit
9.7
fM
(i.e.,
1.94
attomoles)
dynamic
linear
range
4
orders
BMC Genomics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: April 17, 2024
Abstract
Objective
This
study
aims
to
analyze
the
molecular
characteristics
of
novel
coronavirus
(SARS-CoV-2)
Omicron
variant
BA.2.76
in
Jining
City,
China.
Methods
Whole-genome
sequencing
was
performed
on
87
cases
SARS-CoV-2
infection.
Evolutionary
trees
were
constructed
using
bioinformatics
software
sequence
homology,
sites,
N-glycosylation
and
phosphorylation
sites.
Results
All
whole-genome
sequences
classified
under
evolutionary
branch
BA.2.76.
Their
similarity
reference
strain
Wuhan-Hu-1
ranged
from
99.72
99.74%.
In
comparison
Wuhan-Hu-1,
exhibited
77–84
nucleotide
differences
27
deletions.
A
total
69
amino
acid
9
deletions,
1
stop
codon
mutation
identified
across
18
proteins.
Among
them,
spike
(S)
protein
highest
number
ORF8
showed
a
Q27
mutation.
Multiple
proteins
displayed
variations
glycosylation
Conclusion
continues
evolve,
giving
rise
new
strains
with
enhanced
transmission,
stronger
immune
evasion
capabilities,
reduced
pathogenicity.
The
application
high-throughput
technologies
epidemic
prevention
control
COVID-19
provides
crucial
insights
into
virus
at
genomic
level,
thereby
holding
significant
implications
for
pandemic.
