Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

Applied Biological Chemistry, Journal Year: 2023, Volume and Issue: 66(1)

Published: Feb. 18, 2023

CRISPR-Cas systems have been widely used in genome editing and transcriptional regulation. Recently, effectors are adopted for biosensor construction due to its adjustable properties, such as simplicity of design, easy operation, collateral cleavage activity, high biocompatibility. Aptamers' excellent sensitivity, specificity, vitro synthesis, base-pairing, labeling, modification, programmability has made them an attractive molecular recognition element inclusion systems. Here, we review current advances aptamer-based sensors. We briefly discuss aptamers the knowledge Cas effector proteins, crRNA, reporter probes, analytes, applications target-specific aptamers. Next, provide fabrication strategies, binding, detection using fluorescence, electrochemical, colorimetric, nanomaterials, Rayleigh, Raman scattering. The application sensing a wide range biomarkers (disease pathogens) toxic contaminants is growing. This provides update offers novel insights into developing CRISPR-Cas-based sensors ssDNA with efficiency specificity point-of-care setting diagnostics.

Language: Английский

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Advances in Extracellular Vesicle Nanotechnology for Precision Theranostics

Advanced Science, Journal Year: 2022, Volume and Issue: 10(3)

Published: Nov. 14, 2022

Abstract Extracellular vesicles (EVs) have increasingly been recognized as important cell surrogates influencing many pathophysiological processes, including cellular homeostasis, cancer progression, neurologic disease, and infectious disease. These behaviors enable EVs broad application prospects for clinical in disease diagnosis treatment. Many studies suggest that are superior to conventional synthetic carriers terms of drug delivery circulating biomarkers early diagnosis, opening up new frontiers modern theranostics. Despite these potential, containing diverse components, such nucleic acids, proteins, metabolites highly heterogeneous small size. The limitation preparatory, engineering analytical technologies poses technical barriers translation. This article aims at present a critical overview emerging field biomedical applications challenges involved their clinic translations. current methods isolation identification discussed. Additionally, strategies developed enhance scalable production improved cargo loading well tumor targeting presented. potential EVs, particularly different origins the next generation diagnostic treatment platforms, clarified.

Language: Английский

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Electrochemical nano-sensing interface for exosomes analysis and cancer diagnosis

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 214, P. 114554 - 114554

Published: July 10, 2022

Language: Английский

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Electrocatalysis of Copper Sulfide Nanoparticle-Engineered Covalent Organic Frameworks for Ratiometric Electrochemical Detection of Amyloid-β Oligomer

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(32), P. 11201 - 11208

Published: Aug. 3, 2022

Amyloid-β oligomer (AβO) is widely regarded as a reliable biomarker for the early diagnosis of Alzheimer's disease (AD). In this study, signal on-off ratiometric electrochemical immunosensor has been developed ultrasensitive detection AβO. To achieve dual-signal strategy, ultrasmall copper sulfide nanoparticle-engineered covalent organic framework hybrid nanocomposites (CuS@COFs) were utilized excellent electrocatalysts toward hydroquinone (HQ) oxidation to produce detectable signals. Meanwhile, electroactive thionine (Thi) and Aβ antibody-modified gold nanoparticles (Thi-AuNPs-Ab bioconjugates) designed another indicator. Based on these two signals, an sandwich-like was established AβO detection. The introduction resulted in remarkable decline HQ but increase Thi. Under optimum conditions, ratios between double signals (IThi/IHQ) showed proportional linear relationship with concentration (1 pM-1 μM) low limit 0.4 pM (S/N = 3), biosensor able determine content real cerebrospinal fluid samples satisfactory results. strategy proposed our study offers sensitive efficient approach AD, work will promote further applications engineered COFs sensors.

Language: Английский

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Covalent organic framework: A state-of-the-art review of electrochemical sensing applications

Talanta, Journal Year: 2023, Volume and Issue: 260, P. 124613 - 124613

Published: April 29, 2023

Language: Английский

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Nanoplatforms Based on Covalent Organic Frameworks (COFs) for Biomedical Applications

Chemistry of Materials, Journal Year: 2023, Volume and Issue: 35(20), P. 8353 - 8370

Published: Oct. 12, 2023

Covalent organic frameworks (COFs) make up an emerging class of crystalline porous materials mainly composed light elements in the form dynamic covalent bonds. Owing to their two- or three-dimensional network structures and ideal properties, including low density, large specific surface area, high chemical stability, good biocompatibility, COFs have shown a wide range applications optoelectronic devices, energy conversion storage, adsorption separation, sensing, catalysis, biomedicine. This review provides overview recent advances functional COF-based nanoplatforms for biological diagnosis treatment, such as enzyme protein drug delivery, photodynamic/photothermal therapy, synergistic treatment. Challenges future directions developing biomedicine related are also discussed. We envisage that this will inspire scientists, chemists, biologists, clinical doctors working fields work closely move field toward trials human healthcare.

Language: Английский

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State-of-the-art electrochemical biosensors based on covalent organic frameworks and their hybrid materials

Talanta, Journal Year: 2023, Volume and Issue: 270, P. 125557 - 125557

Published: Dec. 16, 2023

Language: Английский

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Polydopamine-assisted aptamer-carrying tetrahedral DNA microelectrode sensor for ultrasensitive electrochemical detection of exosomes

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 8, 2024

Abstract Background Exosomes are nanoscale extracellular vesicles (30–160 nm) with endosome origin secreted by almost all types of cells, which considered to be messengers intercellular communication. Cancerous exosomes serve as a rich source biomarkers for monitoring changes in cancer-related physiological status, because they carry large number biological macromolecules derived from parental tumors. The ultrasensitive quantification trace amounts cancerous is highly valuable non-invasive early cancer diagnosis, yet it remains challenging. Herein, we developed an aptamer-carrying tetrahedral DNA (Apt-TDNA) microelectrode sensor, assisted polydopamine (PDA) coating semiconducting properties, the electrochemical detection cancer-derived exosomes. Results stable rigid structure and orientation Apt-TDNA ensured efficient capture suspended Without PDA signal amplification strategy, sensor has linear working range 10 2 –10 7 particles mL −1 , LOD ~ 69 42 EIS DPV, respectively. With coating, further amplified, achieving single particle level sensitivity (~ 14 6 DPV). Conclusions proposed PDA-assisted integrates exosome capture, sensitive feedback amplification, provides new avenue development simple sensing techniques diagnosis treatment response. Graphical

Language: Английский

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CRISPR integrated biosensors: A new paradigm for cancer detection

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

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Signal-amplification strategy and advances in electrochemistry-based CRISPR/Cas12 biosensing

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161110 - 161110

Published: Feb. 1, 2025

Language: Английский

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