Application of fluorescence i-motif DNA silver nanocluster sensor to visualize endogenous reactive oxygen species in plant cells DOI Creative Commons

Young Kyoung Oh,

Hari Chandana Yadavalli,

Moon Young Ryu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Reactive oxygen species (ROS) are crucial in plant growth, defense, and stress responses, making them vital for improving crop resilience. Various ROS sensing methods plants have been developed to detect in vitro vivo. However, each method comes its own advantages disadvantages, leading an increasing demand a simple effective sensory system detection plants. Here, we introduce novel DNA silver nanoclusters (DNA/AgNCs) sensors visualizing Two sensors, C20/AgNCs FAM-C20/AgNCs-Cy5, intracellular signaling response stimuli such as abscisic acid, salicylic ethylene, bacterial peptide elicitor flg22. Notably, FAM-C20/AgNCs-Cy5 exceeds the capabilities of HyPer7, widely recognized sensor. Taken together, suggest that fluorescent i-motif DNA/AgNCs is tool signals cells. This advancement important advancing our understanding ROS-mediated processes biology.

Language: Английский

Laser ablated Au@Ag@Au core double shelled as inorganic nanocomposite for control growth of liver cancer cells (Hep-G2): 2D tissue culture model and insilico assessment DOI

Ahmed A. Aktafa,

Uday M. Nayef, Majid S. Jabir

et al.

Inorganic Chemistry Communications, Journal Year: 2025, Volume and Issue: unknown, P. 113841 - 113841

Published: Jan. 1, 2025

Language: Английский

Citations

2
Advancements in liposomal formulations: A comprehensive exploration of industrial production techniques DOI
Mohammad Sameer Khan, Garima Gupta,

Abdulrhman Alsayari

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 658, P. 124212 - 124212

Published: May 8, 2024

Language: Английский

Citations

10
Nanoplasmonic sensing as a rapid and sensitive methodology to investigate tolvaptan loaded plant-derived nanovesicles and liposomes DOI Creative Commons
Shishir Jaikishan, Ramila Mammadova, Rui Chen

et al.

Journal of Pharmaceutical and Biomedical Analysis Open, Journal Year: 2025, Volume and Issue: unknown, P. 100052 - 100052

Published: Jan. 1, 2025

Language: Английский

Citations

0
Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment DOI Creative Commons
Aleksandra Steć, Monika Targońska, Shishir Jaikishan

et al.

Drug Delivery, Journal Year: 2024, Volume and Issue: 32(1)

Published: Dec. 11, 2024

Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources EVs currently being explored to overcome limitations related their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived were tested as carriers for the widely used chemotherapeutic – doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) developed quality control DOX–EV preparations. It was found that CE method enables simultaneous detection free incorporated DOX allows assessing stability preparations leakage. NPS, on other hand, demonstrated is accumulated in interfacial region carrier. The activity DOX-loaded HeLa (cervical cancer cells) HEK293T (human embryonic kidney cell lines. incorporation into plant-derived virtually does not affect drug's cytotoxicity cells but significantly decreases against line.

Language: Английский

Citations

3
Fragment-based approach to study fungicide-biomimetic membrane interactions DOI Creative Commons
Shishir Jaikishan, Marine Lavainne, Henri Ravald

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: 20(30), P. 5954 - 5968

Published: Jan. 1, 2024

Molecular interactions of the allylamine-type fungicide butenafine and a set substructures with biomimetic liposomes were studied to gain better understanding structural factors governing membrane affinity perturbation.

Language: Английский

Citations

2
Application of fluorescence i-motif DNA silver nanocluster sensor to visualize endogenous reactive oxygen species in plant cells DOI

Young Kyoung Oh,

Hari Chandana Yadavalli,

Moon Young Ryu

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 44(1)

Published: Dec. 15, 2024

Language: Английский

Citations

1
Application of fluorescence i-motif DNA silver nanocluster sensor to visualize endogenous reactive oxygen species in plant cells DOI Creative Commons

Young Kyoung Oh,

Hari Chandana Yadavalli,

Moon Young Ryu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Reactive oxygen species (ROS) are crucial in plant growth, defense, and stress responses, making them vital for improving crop resilience. Various ROS sensing methods plants have been developed to detect in vitro vivo. However, each method comes its own advantages disadvantages, leading an increasing demand a simple effective sensory system detection plants. Here, we introduce novel DNA silver nanoclusters (DNA/AgNCs) sensors visualizing Two sensors, C20/AgNCs FAM-C20/AgNCs-Cy5, intracellular signaling response stimuli such as abscisic acid, salicylic ethylene, bacterial peptide elicitor flg22. Notably, FAM-C20/AgNCs-Cy5 exceeds the capabilities of HyPer7, widely recognized sensor. Taken together, suggest that fluorescent i-motif DNA/AgNCs is tool signals cells. This advancement important advancing our understanding ROS-mediated processes biology.

Language: Английский

Citations

0