Stem Cell Reports,
Journal Year:
2021,
Volume and Issue:
16(5), P. 1078 - 1092
Published: May 1, 2021
Post-implantation
embryo
development
commences
with
a
bilaminar
disc
in
most
mammals,
including
humans.
Whereas
access
to
early
human
embryos
is
limited
and
subject
greater
ethical
scrutiny,
studies
on
non-primate
developing
as
discs
offer
exceptional
opportunities
for
advances
gastrulation,
the
germline,
basis
evolutionary
divergence
applicable
development.
Here,
we
discuss
advantages
of
investigations
pig
an
exemplar
relevance
Besides,
has
potential
creation
humanized
organs
xenotransplantation.
Precise
genetic
engineering
approaches,
imaging,
single-cell
analysis
are
cost
effective
efficient,
enabling
research
into
some
outstanding
questions
authentic
models
stem
cells.
Development,
Journal Year:
2017,
Volume and Issue:
144(3), P. 365 - 373
Published: Jan. 31, 2017
ABSTRACT
The
regulative
capability
of
single
cells
to
give
rise
all
primary
embryonic
lineages
is
termed
pluripotency.
Observations
fluctuating
gene
expression
and
phenotypic
heterogeneity
in
vitro
have
fostered
a
conception
pluripotency
as
an
intrinsically
metastable
precarious
state.
However,
the
embryo
defined
culture
environments
properties
pluripotent
change
orderly
sequence.
Two
phases
pluripotency,
called
naïve
primed,
previously
been
described.
In
this
Hypothesis
article,
third
phase,
formative
proposed
exist
part
developmental
continuum
between
primed
phases.
phase
hypothesised
be
enabling
for
execution
entailing
remodelling
transcriptional,
epigenetic,
signalling
metabolic
networks
constitute
multi-lineage
competence
responsiveness
specification
cues.
Cell stem cell,
Journal Year:
2020,
Volume and Issue:
28(3), P. 453 - 471.e8
Published: Dec. 2, 2020
Pluripotent
cells
emerge
as
a
naive
founder
population
in
the
blastocyst,
acquire
capacity
for
germline
and
soma
formation,
then
undergo
lineage
priming.
Mouse
embryonic
stem
(ESCs)
epiblast-derived
(EpiSCs)
represent
initial
final
primed
phases
of
pluripotency,
respectively.
Here,
we
investigate
intermediate
formative
stage.
Using
minimal
exposure
to
specification
cues,
derive
from
mouse
epiblast.
Unlike
ESCs
or
EpiSCs,
(FS)
respond
directly
germ
cell
induction.
They
colonize
somatic
tissues
chimeras.
Whole-transcriptome
analyses
show
similarity
pre-gastrulation
Signal
responsiveness
chromatin
accessibility
features
reflect
capacitation.
Furthermore,
FS
distinct
transcription
factor
dependencies,
relying
critically
on
Otx2.
Finally,
culture
conditions
applied
human
embryos
support
expansion
similar
cells,
consistent
with
conserved
staging
post
trajectory
mammalian
pluripotency.
Nature Communications,
Journal Year:
2019,
Volume and Issue:
10(1)
Published: Feb. 5, 2019
Abstract
Regeneration
of
human
kidneys
in
animal
models
would
help
combat
the
severe
shortage
donors
transplantation
therapy.
Previously,
we
demonstrated
by
interspecific
blastocyst
complementation
between
mouse
and
rats,
generation
pluripotent
stem
cell
(PSC)-derived
functional
pancreas,
apancreatic
Pdx1
mutant
mice.
We,
however,
were
unable
to
obtain
rat
PSC-derived
anephric
Sall1
mice,
likely
due
poor
contribution
PSCs
metanephric
mesenchyme,
a
nephron
progenitor.
Here,
conversely,
show
that
can
efficiently
differentiate
into
mesenchyme
rat,
allowing
kidney
rat.
Glomerular
epithelium
renal
tubules
are
entirely
composed
cells
expressing
key
markers.
Importantly,
ureter-bladder
junction
is
normally
formed.
These
data
provide
proof-of-principle
for
as
viable
approach
generation.
Cell Research,
Journal Year:
2021,
Volume and Issue:
31(5), P. 526 - 541
Published: Feb. 19, 2021
Abstract
The
pluripotency
of
mammalian
early
and
late
epiblast
could
be
recapitulated
by
naïve
embryonic
stem
cells
(ESCs)
primed
(EpiSCs),
respectively.
However,
these
two
states
may
not
sufficient
to
reflect
the
full
complexity
developmental
potency
during
development.
Here
we
report
establishment
self-renewing
formative
pluripotent
(fPSCs)
which
manifest
features
poised
for
gastrulation.
fPSCs
can
established
from
different
mouse
ESCs,
pre-/early-gastrula
epiblasts
induced
PSCs.
Similar
epiblasts,
show
transcriptomic
pluripotency,
are
distinct
ESCs
EpiSCs.
unique
epigenetic
E6.5
epiblast,
including
super-bivalency
a
large
set
genes.
Just
like
immediately
before
gastrulation,
efficiently
differentiate
into
three
germ
layers
primordial
(PGCs)
in
vitro.
Thus,
highlight
feasibility
using
PSCs
explore
development
epiblast.
Development Growth & Differentiation,
Journal Year:
2018,
Volume and Issue:
60(1), P. 44 - 52
Published: Jan. 1, 2018
Pluripotency
denotes
the
flexible
capacity
of
single
cells
to
give
rise
all
somatic
lineages
and
typically
also
germline.
Mouse
ES
post-implantation
epiblast-derived
stem
(EpiSC)
are
widely
used
pluripotent
cell
culture
systems.
These
two
in
vitro
types
have
divergent
characteristics.
They
considered
as
representative
distinct
developmental
stages,
distinguished
by
using
terms
"naïve"
"primed".
A
binary
description
is
an
over-simplification,
however.
Here,
we
discuss
intermediate
stage
pluripotency
that
term
"formative".
Formative
features
a
gene
regulatory
network
switch
from
naïve
state
comprises
capacitation
enhancers,
signaling
pathways
epigenetic
machinery
order
install
competence
for
lineage
specification.
