Interspecies Chimerism with Mammalian Pluripotent Stem Cells

Cell, Journal Year: 2017, Volume and Issue: 168(3), P. 473 - 486.e15

Published: Jan. 1, 2017

Language: Английский

---

Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency

Cell, Journal Year: 2017, Volume and Issue: 169(2), P. 243 - 257.e25

Published: April 1, 2017

Language: Английский

---

Tissue repair and regeneration with endogenous stem cells

Nature Reviews Materials, Journal Year: 2018, Volume and Issue: 3(7), P. 174 - 193

Published: June 19, 2018

In contrast to non-mammalian vertebrates, mammals and humans have limited innate capacity for the self-regeneration of tissues organs owing differences in genetics, development, immune systems tissue complexity. Endogenous stem cells are tissue-specific adult with self-renew differentiate into specific cell types. Therefore, endogenous being explored regeneration situ vivo. Stem reside niches body, activation depends on progressive changes niche. Niches instructive microenvironments that can be recreated using biomaterial-based scaffolds. Such scaffolds fabricated a variety shapes formulations, they functionalized biochemical biophysical cues guide fate migration. this Review, we discuss important abilities vertebrates mammals, including humans, investigate populations their involved repair regeneration. We highlight natural synthetic biomaterials potential improving applications examine role interspecies chimaeras regenerative medicine. play key remodelling body. This Review discusses different investigates approaches combination biomaterials.

Language: Английский

---

Research and therapy with induced pluripotent stem cells (iPSCs): social, legal, and ethical considerations

Stem Cell Research & Therapy, Journal Year: 2019, Volume and Issue: 10(1)

Published: Nov. 21, 2019

Abstract Induced pluripotent stem cells (iPSCs) can self-renew indefinitely in culture and differentiate into all specialized cell types including gametes. iPSCs do not exist naturally are instead generated (“induced” or “reprogrammed”) from somatic through ectopic co-expression of defined pluripotency factors. Since they be any healthy person patient, considered as a valuable resource for regenerative medicine to replace diseased damaged tissues. In addition, reprogramming technology has provided powerful tool study mechanisms fate decisions model human diseases, thereby substantially potentiating the possibility (i) discover new drugs screening formats (ii) treat life-threatening diseases therapy-based strategies. However, various legal ethical barriers arise when aiming exploit full potential minimize abuse unauthorized utilization. this review, we discuss bioethical, legal, societal concerns associated with research therapy using iPSCs. Furthermore, present key questions suggestions scientists, authorities, social activists investigating working field.

Language: Английский

---

Xenotransplantation: Current Status in Preclinical Research

Frontiers in Immunology, Journal Year: 2020, Volume and Issue: 10

Published: Jan. 23, 2020

The increasing life expectancy of humans has led to growing numbers patients with chronic diseases and end-stage organ failure. Transplantation is an effective approach for the treatment failure; however, imbalance between supply demand human organs a bottleneck clinical transplantation. Therefore, xenotransplantation might be promising alternative bridge gap organs, tissues, cells; immunological barriers are limiting factors in xenotransplantation. Thanks advances gene-editing tools immunosuppressive therapy as well prolonged xenograft survival time pig-to-nonhuman primate (NHP) models, become more viable. In this review, we focus on evolution current status research, including our understanding mechanisms involved rejection, genetically modified pigs used xenotransplantation, progress that been made developing pig-to-NHP models. Three main types rejection can occur after which discuss detail: (1) hyperacute (2) acute humoral (3) cellular rejection. Furthermore, studies have generated cross-species molecular incompatibilities; last decade, most field resulted from production engineered pigs; accordingly, summarize currently available Next, longest solid preclinical models recent years, heart, liver, kidney, lung Overall, conclude achievements accumulation experience mean first-in-human trial could possible near future. hope various approaches will able collectively solve problem shortage.

Language: Английский

---

Regenerative medicine, organ bioengineering and transplantation

British journal of surgery, Journal Year: 2020, Volume and Issue: 107(7), P. 793 - 800

Published: May 28, 2020

Abstract Background Organ transplantation is predicted to increase as life expectancy and the incidence of chronic diseases rises. Regenerative medicine-inspired technologies challenge efficacy current allograft model. Methods A literature review was conducted using PubMed interface MEDLINE from National Library Medicine. Results were examined for relevance innovations organ bioengineering inform analysis advances in regenerative medicine affecting transplantation. Data reports Scientific Registry Transplant Recipient Procurement Transplantation Network 2008 2019 kidney, pancreas, liver, heart, lung intestine transplants performed, patients currently on waiting lists respective organs, reviewed demonstrate shortage need transplantable organs. aim repair regenerate poorly functioning One goal achieve an immunosuppression-free state improve quality life, reduce complications toxicities, eliminate cost lifelong antirejection therapy. Innovative strategies include decellularization fabricate acellular scaffolds that will be used a template manufacturing, three-dimensional printing interspecies blastocyst complementation. Induced pluripotent stem cells are innovation cell technology which mitigate both ethical concerns associated with embryonic limitation other progenitor cells, lack pluripotency. hold promise wide array fields applications, such promoting regeneration native lines, growth new tissue or modelling disease states, augmenting viability existing ex vivo transplanted Conclusion The future relies furthering understanding organogenesis, regeneration, immunology long-term monitoring implanted bioengineered

Language: Английский

---

Hallmarks of totipotent and pluripotent stem cell states

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(3), P. 312 - 333

Published: Feb. 20, 2024

Though totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for development of all mammals. Studying these in vivo has been challenging due to limited access ethical constraints, particularly humans. Recent progress led diverse culture adaptations epiblast cells vitro form totipotent pluripotent stem cells, which not only deepen our understanding embryonic but also serve as invaluable resources animal reproduction regenerative medicine. This review delves into hallmarks shedding light on their key molecular functional features.

Language: Английский

---

Stem Cells, Genome Editing, and the Path to Translational Medicine

Cell, Journal Year: 2018, Volume and Issue: 175(3), P. 615 - 632

Published: Oct. 1, 2018

Language: Английский

---

Capturing Totipotent Stem Cells

Cell stem cell, Journal Year: 2018, Volume and Issue: 22(1), P. 25 - 34

Published: Jan. 1, 2018

Language: Английский

---

Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo

Cell, Journal Year: 2021, Volume and Issue: 184(8), P. 2020 - 2032.e14

Published: April 1, 2021

Language: Английский

---