bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 22, 2024
Regeneration and homeostatic turnover of solid tissues depend on the proliferation symmetrically dividing adult stem cells, which either remain cells or differentiate based their niche position. Here we demonstrate that in zebrafish lateral line sensory organs, progenitor cell are independently regulated by two
Language: Английский
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Sept. 12, 2024
To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework ensure consistency and reliability in development, characterization, organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, control measures, functional assessments, aimed at fostering approach across diverse research initiatives. implementation these anticipated significantly contribute reproducibility comparability results burgeoning field organoid research.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 15, 2024
Abstract Tissue maintenance is underpinned by resident stem cells whose activity modulated microenvironmental cues. Using Drosophila as a simple model to identify regulators of cell behaviour and survival in vivo , we have identified novel connections between the conserved transmembrane proteoglycan Syndecan, nuclear properties function. In midgut, Syndecan depletion intestinal results their loss from tissue, impairing tissue renewal. At cellular level, alters shape, causes lamina invaginations DNA damage. second developing brain, live imaging revealed that neural envelope remodelling defects which arise upon division. Our findings reveal new role for diverse types. Graphical
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8815 - 8815
Published: Aug. 13, 2024
The adult mammalian heart has been demonstrated to be endowed with low but real turnover capacity, especially for cardiomyocytes, the key functional cell type. source, however, of that capacity remains controversial. In this regard, we have defined and characterized a resident multipotent cardiac mouse progenitor population, Bmi1+DR (for Bmi1+ Damage-Responsive cells). is one types lowest ROS (Reactive Oxygen Species) levels in heart, being particularly by their close relationship vessels, most probably involved regulation proliferation/maintenance Bmi1+DR. This was proposed work as endothelial niche. Due scarcity cells generated an immortalization/dis-immortalization model using Simian Vacuolating Virus 40-Large Antigen T (SV40-T) facilitate vitro characterization. We obtained heterogeneous population immortalized (Bmi1+DRIMM) validated attending different criteria, also showing comparable sensitivity strong oxidative damage. Then, concluded Bmi1-DRIMM appropriate primary studies. co-culture Bmi1+DRIMM protects them against damage, moderate depletion non-canonical autophagy contributing modest metabolic regulation.
Language: Английский
Citations
0
Published: Oct. 19, 2024
Abstract The histone methylase Setdb1 plays a pivotal role in embryonic stem cell maintenance and developmental lineage specification. However, its function adult cells remains elusive. Here we show that conditional inactivation of Lgr5 + intestinal alters the transcriptional programs progeny types results increased cell-to-cell variability. Loss blocked differentiation towards absorptive enterocyte lineage, while secretory were only marginally affected due to activation alternative trajectories. did not alter H3K9 methylation at large heterochromatin domains but led reduced localized modifications islands gene-rich areas around transposase-accessible chromatin regions, which doubled number, became broader more heterogeneous size. demonstrate regulates by fine-tuning accessibility open euchromatin regions thereby controlling variability between cells.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 22, 2024
Regeneration and homeostatic turnover of solid tissues depend on the proliferation symmetrically dividing adult stem cells, which either remain cells or differentiate based their niche position. Here we demonstrate that in zebrafish lateral line sensory organs, progenitor cell are independently regulated by two
Language: Английский
Citations
0