Guanine-Based DNA Biosensor Amplified with Pt/SWCNTs Nanocomposite as Analytical Tool for Nanomolar Determination of Daunorubicin as an Anticancer Drug: A Docking/Experimental Investigation

Industrial & Engineering Chemistry Research, Journal Year: 2021, Volume and Issue: 60(2), P. 816 - 823

Published: Jan. 8, 2021

Daunorubicin is a famous anthracycline anticancer chemotherapy drug with many side effects that very important to measure in biological samples. A daunorubicin electrochemical biosensor was fabricated this study using ds-DNA as the biorecognition element and glassy carbon electrode (GCE) amplified by Pt/SWCNTs sensor. The synthetization of done polyol method, their characterization accomplished via XRD, EDS, TEM methods. results showed diameter about 5.0 nm for Pt nanoparticle decorated at surface SWCNTs. morphological conductivity properties Pt/SWCNTs/GCE were investigated EIS AFM methods, confirmed had high area conductivity. ds-DNA/Pt/SWCNTs/GCE an oxidation signal relative guanine base potential 847 mV positive shift after interaction drug. This point confirms intercalation reaction between structure could be used analytical factor determination daunorubicin. Furthermore, molecular docking predict site DNA. It found interacts bases DNA intercalative mode. Kinetic investigation association equilibrium constant (Ka) 5.044 × 103 M–1 differential pulse voltammetric linear dynamic range 4.0 nM 250.0 μM detection limit 1.0 on ds-DNA/Pt/SWCNTs/GCE. Finally, successfully injection samples recovery 98.27–10313%.

Language: Английский

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Cyanazine herbicide monitoring as a hazardous substance by a DNA nanostructure biosensor

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 423, P. 127058 - 127058

Published: Aug. 28, 2021

Language: Английский

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Review—Measurement and Analysis of Cancer Biomarkers Based on Electrochemical Biosensors

Journal of The Electrochemical Society, Journal Year: 2020, Volume and Issue: 167(3), P. 037525 - 037525

Published: Jan. 2, 2020

Cancer is a dreadful disease with high mortality rate, and it has become more prevalent worldwide. Early diagnosis, prognosis treatment monitoring robust non-invasive tools will potentially be the future focus. Electrochemical biosensor can strong candidate for cancer theranostics owing to their advantage of ultra-sensitivity, selectivity, low cost, quick readout, simplicity. Furthermore, electrochemical biosensors are easier miniaturized mass fabricated, which grant them better fit point-of-care applications. In this review, various measurement methods, bioreceptor surface, signal generation amplification, integration sensors in microfluidic chips were summarized. Especially, multiplexed ratiometric emphasized biomarkers detection. Then, analysis cancers based on molecular level (DNA, RNA, protein), organelle (exosomes), cell (cell counting, phenotypic metabolism analysis, drug sensitivity monitoring) comprehensively discussed. As new research trend, cancer-on-a-chip been highlighted. brief, we present an overall review current advances using biosensors. Finally, challenges directions

Language: Английский

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Metal–organic frameworks (MOFs) based electrochemical biosensors for early cancer diagnosis in vitro

Coordination Chemistry Reviews, Journal Year: 2021, Volume and Issue: 439, P. 213948 - 213948

Published: April 24, 2021

Language: Английский

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Oriented immobilization of antibodies onto sensing platforms - A critical review

Analytica Chimica Acta, Journal Year: 2021, Volume and Issue: 1189, P. 338907 - 338907

Published: Aug. 4, 2021

The immunosensor has been proven a versatile tool to detect various analytes, such as food contaminants, pathogenic bacteria, antibiotics and biomarkers related cancer. To fabricate robust reproducible immunosensors with high sensitivity, the covalent immobilization of immunoglobulins (IgGs) in site-specific manner contributes better performance. Instead random IgG orientations result from direct yet non-selective techniques, this review for first time introduces advances stepwise site-selective conjugation strategies give biosensing efficiency. Noncovalently adsorbing IgGs is but decisive step interact specifically Fc fragment, then following conjugate can fix uniform antigens-favorable orientation irreversibly. In review, we categorized strategy into two parts based on different noncovalent interactions, namely adhesive layer-mediated interaction onto homofunctional support layer-free heterofunctional (which displays several functionalities its surface that are capable IgGs). Further, influence ligands characteristics (synthesis strategies, spacer requirements matrices selection) also discussed. Finally, conclusions future perspectives real-world application This provides more insights fabrication high-efficiency immunosensor, special attention devoted well-orientation full-length sensing platform.

Language: Английский

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Advances in surface plasmon resonance–based biosensor technologies for cancer biomarker detection

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 197, P. 113767 - 113767

Published: Nov. 3, 2021

Language: Английский

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Recent development in electrochemical biosensors for cancer biomarkers detection

Biosensors and Bioelectronics X, Journal Year: 2021, Volume and Issue: 8, P. 100075 - 100075

Published: July 10, 2021

Cancer is the most frequent life-threatening disease which has highest mortality rate throughout world. Diagnosis of cancer at early stage can plays a critical role for its effective and successful treatment. Traditional diagnostic methods screening are costly, time-consuming, not practical repeated screenings. However, biomarker-based diagnosis emerging as one promising strategies diagnosis, monitoring progression, subsequent This review describes recent advances improvements in electrochemical biosensors designed detecting various biomarkers using different signal transduction techniques biological recognition strategies.

Language: Английский

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Fabricated Metal-Organic Frameworks (MOFs) as luminescent and electrochemical biosensors for cancer biomarkers detection

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 197, P. 113738 - 113738

Published: Oct. 29, 2021

Language: Английский

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Surface Plasmon Resonance (SPR) Sensor for Cancer Biomarker Detection

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 396 - 396

Published: March 17, 2023

A biomarker is a physiological observable marker that acts as stand-in and, in the best-case scenario, forecasts clinically significant outcome. Diagnostic biomarkers are more convenient and cost-effective than directly measuring ultimate clinical Cancer among most prominent global health problems major cause of morbidity death globally. Therefore, cancer assays trustworthy, consistent, precise, verified desperately needed. Biomarker-based tumor detection holds lot promise for improving disease knowledge at molecular scale early surveillance. In contrast to conventional approaches, surface plasmon resonance (SPR) allows quick less invasive screening variety circulating indicators, such DNA (ctDNA), microRNA (miRNA), cells (CTCs), lipids, proteins. With several advantages, SPR technique particularly beneficial choice point-of-care identification biomarkers. As result, it enables timely markers, which could be used track development suppress relapse malignant tumors. This review emphasizes advancements biosensing technologies detection.

Language: Английский

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State-of-the-art cancer biomarker detection by portable (Bio) sensing technology: A critical review

Microchemical Journal, Journal Year: 2022, Volume and Issue: 177, P. 107248 - 107248

Published: Feb. 9, 2022

Language: Английский

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