International Journal of Peptide Research and Therapeutics, Journal Year: 2024, Volume and Issue: 31(1)
Published: Nov. 16, 2024
Language: Английский
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117629 - 117629
Published: March 5, 2024
Language: Английский
Citations
20
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(24), P. 10064 - 10073
Published: June 6, 2024
The global spread of monkeypox has become a worldwide public healthcare issue. Therefore, there is an urgent need for accurate and sensitive detection methods to effectively control its spreading. Herein, we screened by phage display two peptides M4 (sequence: DPCGERICSIAL) M6 SCSSFLCSLKVG) with good affinity specificity virus (MPXV) B21R protein. To simulate the state peptide in avoid spatial obstacles peptide, GGGSK was added at C terminus named as M4a. Molecular docking shows that M4a are bound different epitopes hydrogen bonds salt-bridge interactions, respectively. Then, selected capture probe, carbonized polymer dots (CPDs) fluorescent colorimetric double-signal peptide/antigen/signal peptide-displayed sandwich ELISA triggered horseradish peroxidase (HRP) through simple internal filtration effect (IFE) constructed. HRP catalyzes H2O2 oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) generate blue oxidized TMB, which can further quench fluorescence CPDs IFE, enabling detect MPXV modes. proposed immunoassay platform sensitivity reliability detection. limit modes 27.8 9.14 pg/mL B21R, Thus, established double-peptide sandwich-based dual-signal provides guidance development reliable antigen capable mutual confirmation, also great potential exploring various analytical strategies other respiratory surveillance.
Language: Английский
Citations
9
Micromachines, Journal Year: 2024, Volume and Issue: 15(2), P. 181 - 181
Published: Jan. 25, 2024
Impedimetric biosensors represent a powerful and promising tool for studying monitoring biological processes associated with proteins can contribute to the development of new approaches in diagnosis treatment diseases. The basic principles, analytical methods, applications hybrid impedimetric express protein detection fluids are described. advantages this type biosensors, such as simplicity speed operation, sensitivity selectivity analysis, cost-effectiveness, an ability be integrated into microfluidic systems, demonstrated. Current challenges prospects area analyzed. They include (a) selection materials electrodes formation nanostructures on their surface; (b) efficient methods biorecognition elements’ deposition electrodes’ surface, providing specificity biosensing; (c) reducing nonspecific binding interference, which could affect specificity; (d) adapting real samples conditions operation; (e) expanding range detected proteins; and, finally, (f) biosensor integration large microanalytical system technologies. This review useful researchers working field detection, well those interested application biomedical diagnostics.
Language: Английский
Citations
6
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 497, P. 154826 - 154826
Published: Aug. 13, 2024
Language: Английский
Citations
6
Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4551 - 4551
Published: Sept. 25, 2024
Immunoassays have been widely used for the determination of various analytes in fields disease diagnosis, food safety, and environmental monitoring. Dual-signal immunoassays are now advanced integrated detection technologies with excellent self-correction self-validation capabilities. In this work, we summarize recent advances development optical electrochemical dual-signal immunoassays, including colorimetric, fluorescence, surface-enhanced Raman spectroscopy (SERS), electrochemical, electrochemiluminescence, photoelectrochemical methods. This review particularly emphasizes working principle diverse utilization dual-functional molecules nanomaterials. It also outlines challenges prospects future research on immunoassays.
Language: Английский
Citations
6
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 171, P. 117481 - 117481
Published: Dec. 12, 2023
Language: Английский
Citations
11
Biosensors and Bioelectronics X, Journal Year: 2024, Volume and Issue: 20, P. 100531 - 100531
Published: Aug. 23, 2024
Language: Английский
Citations
2
Microchemical Journal, Journal Year: 2023, Volume and Issue: 196, P. 109634 - 109634
Published: Nov. 18, 2023
Language: Английский
Citations
4
Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11
Published: Feb. 7, 2024
The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), which causes the disease 2019 (COVID-19), is spreading worldwide. Although COVID-19 epidemic has passed its peak of transmission, harm it caused deserves our attention. Scientists are striving to develop medications that can effectively treat symptoms without causing any adverse reactions. SARS-CoV-2 inhibitory peptides derived from animal proteins have a wide range functional activities in addition safety. Identifying protein sources crucial obtaining sources. This review aims reveal mechanisms action these on and possibility as material source peptides. Also, introduces utilization computer-aided design methods, phage display, drug delivery strategies research inhibitor proteins. In order identify new antiviral boost their efficiency, we recommend investigating interaction between non-structural (Nsps) using variety technologies, including approaches, display techniques, techniques. article provides useful information for development novel anti-COVID-19 drugs.
Language: Английский
Citations
0
International Journal of Peptide Research and Therapeutics, Journal Year: 2024, Volume and Issue: 31(1)
Published: Nov. 16, 2024
Language: Английский
Citations
0