International Journal of Mosquito Research,
Journal Year:
2024,
Volume and Issue:
11(1), P. 92 - 98
Published: Jan. 1, 2024
The
recent
developments
of
viral
illnesses
are
vector-
borne,
such
as
Chikungunya
and
dengue
fever,
other
similar
diseases,
have
raised
significant
global
concern.
In
this
paper,
we
provide
a
comprehensive
overview
the
existing
literature
pertaining
to
transmission,
clinical
manifestations,
diagnostic
methods,
impact,
likelihood
future
outbreaks
associated
with
these
pathogens.
Arboviruses
difficult
identify
can
cause
unexpected
consequences.
Dengue
chikungunya
most
common
arboviruses
caused
infections
worldwide,
particularly
in
tropical
subtropical
areas.
transmission
diseases
human
beings
is
facilitated
by
Aedes
aegypti
albopictus
mosquito
species.
spread
mostly
responsible
for
rise
cases
India.
expeditious
accurate
identification
utmost
importance
effectively
managing
containing
epidemics
disease.
light
lack
an
available
vaccine
or
targeted
pharmaceutical
intervention
viruses,
strategy
currently
vector
control.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(38)
Published: Aug. 9, 2024
Dual-mode
readout
platforms
with
colorimetric
and
electrochemiluminescence
(ECL)
signal
enhancement
are
proposed
for
the
ultrasensitive
flexible
detection
of
monkeypox
virus
(MPXV)
in
different
scenes.
A
new
nanotag,
Ru@U6-Ru/Pt
NPs
is
constructed
dual-mode
by
integrating
double-layered
ECL
luminophores
nanozyme
using
Zr-MOF
(UiO-66-NH
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(21), P. 8665 - 8673
Published: May 9, 2024
Prostate-specific
antigen
(PSA)
is
a
key
marker
for
prostate
cancer
diagnosis.
The
low
sensitivity
of
traditional
lateral
flow
immunoassay
(LFIA)
methods
makes
them
unsuitable
point-of-care
testing.
Herein,
we
designed
nanozyme
by
in
situ
growth
Prussian
blue
(PB)
within
the
pores
dendritic
mesoporous
silica
(DMSN).
PB
was
forcibly
dispersed
into
DMSN,
leading
to
an
increase
exposed
active
sites.
Consequently,
atom
utilization
enhanced,
resulting
superior
peroxidase
(POD)-like
activity
compared
that
cubic
PB.
Antibody-modified
DMSN@PB
nanozymes
serve
as
immunological
probes
enzymatic-enhanced
colorimetric
and
photothermal
dual-signal
LFIA
PSA
detection.
After
systematic
optimization,
based
on
successfully
achieves
4-fold
amplification
signal
7
min
through
catalytic
oxidation
chromogenic
substrate
POD-like
activity.
Moreover,
exhibits
excellent
conversion
ability
under
808
nm
laser
irradiation.
Accordingly,
signals
are
introduced
improve
anti-interference
LFIA,
exhibiting
wide
linear
range
(1–40
ng
mL–1)
detection
limit
(0.202
mL–1),
which
satisfies
early
level
cancer.
This
research
provides
more
accurate
reliable
visualization
analysis
methodology
diagnosis
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 7, 2025
Mycotoxins,
a
category
of
fungal
secondary
metabolites,
frequently
contaminate
food
products
and
pose
severe
threat
to
human
health.
Aspergillus,
genus
fungi,
is
capable
producing
mycotoxins,
with
aflatoxins
(AFs)
ochratoxins
being
its
principal
types.
Aspergillus
mycotoxins
can
wide
range
crops
their
derivatives,
such
as
maize,
wheat,
rice,
minor
cereals,
peanuts,
thereby
threatening
feed
safety.
In
the
paper,
related
biosynthesis
genes
multifaceted
pathways
these
are
first
discussed
in
detail,
elucidated
several
global
regulators,
including
growth
conditions,
oxidative
stress,
cell
signal.
Furthermore,
how
shifts
temperature
water
availability,
driven
by
climate
change
(including
rising
temperatures,
increased
heavy
rainfall
frequency,
prolonged
droughts,
elevated
carbon
dioxide
levels),
key
determinants
proliferation
mycotoxin
production
explored.
Finally,
safeguard
animal
health
from
detrimental
impacts
effective
convenient
analytical
techniques
management
strategies
for
detection
prevention
contamination
analyzed.
Overall,
this
review
provides
promising
solutions
which
great
significance
ensuring
security
protecting
people's
lives
ACS Omega,
Journal Year:
2025,
Volume and Issue:
10(2), P. 2184 - 2196
Published: Jan. 8, 2025
Zika
(ZIKV)
and
Dengue
(DENV)
viruses
are
clinically
significant
due
to
their
severe
neurological
hemorrhagic
complications.
Rapid
diagnostics
often
rely
on
nonstructural
proteins
generate
specific
antibodies.
This
study
aimed
produce
IgG
antibodies
from
the
recombinant
ZIKV
protein
plant-expressed
NS2B
for
arbovirus
detection
in
serum
urine
samples.
The
was
expressed
Nicotiana
benthamiana
purified
chromatographically.
Validation
of
as
an
antigen
indirect
immunoassays
demonstrated
95%
sensitivity
100%
specificity
IgM/IgG
ELISA
tests,
enabling
effective
DENV.
Notably,
r-ZIKV-NS2B
identified
positive
DENV
cases
but
failed
detect
negatives,
suggesting
limitations
diagnostics.
Using
a
diagnostic
medium
offers
less
invasive
more
practical
approach,
broadening
test
applicability.
utilized
patient-derived
samples
healthy
spiked
with
exogenous
virus.
Findings
highlight
potential
ZIKV-NS2B
robust
diagnosis
demonstrate
viability
plant-based
systems
production,
advancing
neglected
tropical
diseases.
Diagnosis,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 28, 2025
Abstract
Introduction
Dengue
is
a
viral
infection
caused
by
any
one
of
the
four
related
dengue
virus
(DENV)
serotypes,
1–4.
DENV
single-stranded
RNA
belonging
to
genus
Flavivirus
.
can
cause
range
symptoms,
from
mild
severe
life-threatening
illness.
Currently,
treatment
for
limited
supportive
care,
with
better
outcomes
achieved
through
early
diagnosis.
The
WHO
has
suggested
that
mortality
be
reduced
nearly
zero
implementing
appropriate
clinical
management
strategies,
such
as
laboratory
This
calls
diagnostic
approaches
combine
high
sensitivity
and
specificity,
while
also
being
suitable
point-of-care
testing
(POCT)
in
remote
locations
minimal
staff
training
low
costs.
Content
In
this
paper,
we
outline
limitations
existing
confirmatory
methods,
ELISA
RT-PCR,
which
are
time-consuming,
expensive,
require
skilled
personnel.
We
highlight
alternative
strategies
overcome
these
challenges.
Additionally,
paper
emphasizes
growing
demand
diagnosing
reduce
risk
death,
must
addressed
next-generation
approaches.
Summary
propose
adoption
fluorescence
immunoassay
(FIA)
chemiluminescence
(CLIA),
have
potential
methods.
Outlook
Improvements
diagnosis,
specific
focus
on
identifying
POCT
setting,
help
achieve
goal
deaths
dengue.
Lab on a Chip,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
The
HP-SML
device
was
developed
by
extending
and
angling
microneedles
to
enhance
ISF
extraction
speed,
enabling
user-friendly
diagnosis
using
only
a
minimal
volume
of
interstitial
fluid.
RSC Advances,
Journal Year:
2025,
Volume and Issue:
15(20), P. 15490 - 15496
Published: Jan. 1, 2025
The
PdNP-based
LFIA
for
the
detection
of
Lp-PLA2
address
sensitivity
limitations
colorimetric
LFIA,
due
to
excellent
visual
discriminability
and
target
recognition
ability.