Development of label-free cell tracking for discrimination of the heterogeneous mesenchymal migration DOI Creative Commons

Sota Endo,

S. Yamamoto,

Hiromi Miyoshi

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320287 - e0320287

Published: March 31, 2025

Image-based cell phenotyping is fundamental in both biology and medicine. As cells are dynamic systems, based on static data complemented by extracted from time-dependent characteristics. We developed a label-free automatic tracking method for phase contrast images. examined the possibility of using motility-based discrimination to identify different types mesenchymal migration invasive malignant cancer non-cancer cells. These were cultured plastic tissue culture vessels, motility parameters trajectories with tracking. Correlation analysis these identified characteristic HT1080 fibrosarcoma 3T3-Swiss fibroblast lines. The parameter “sum turn angles,” combined “frequency turns” at shallow angles “migration speed,” proved effective highlighting characteristics It revealed differences their mechanisms generating propulsive forces. requirements characterize included spatiotemporal resolution segmentation tracking, capable detecting polarity changes associated morphological alterations body displacement. With proposed here, curve computed quadratic angles” turns below 30°” gave best performance 94% sensitivity. Cell process related not only but also healing growth. methodology easy use, enabling anyone without professional skills image analysis, large training datasets, or special devices. has potential application broad range applications basic research. Validating expandability this migration, including scheme force generation, an important consideration future study.

Language: Английский

Mechanical signatures in cancer metastasis DOI Creative Commons
Ayushi Agrawal,

Yousef Javanmardi,

Sara A. Watson

et al.

npj Biological Physics and Mechanics., Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 4, 2025

Abstract The cancer metastatic cascade includes a series of mechanical barrier-crossing events, involving the physical movement cells from their primary location to distant organ. This review describes changes that influence tumour proliferation, progression, and metastasis. We identify potential signatures at every step discuss some latest mechanobiology-based therapeutic interventions highlight importance interdisciplinary approaches in diagnosis treatment.

Language: Английский

Citations

3
The Pentose Phosphate Pathway: From Mechanisms to Implications for Gastrointestinal Cancers DOI Open Access
Jincheng Qiao, Zhiqing Yu,

Han Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 610 - 610

Published: Jan. 13, 2025

The pentose phosphate pathway (PPP), traditionally recognized for its role in generating nicotinamide adenine dinucleotide (NADPH) and ribose-5-phosphate (R5P), has emerged as a critical metabolic hub with involvements various gastrointestinal (GI) cancers. PPP plays crucial roles the initiation, development, tumor microenvironment (TME) of GI cancers by modulating redox homeostasis providing precursors nucleotide biosynthesis. Targeting enzymes their regulatory axis been potential strategy anti-GI cancer therapies. In this review, we summarize mechanisms enzymes, elucidate relationships between TME's elements, discuss therapeutic targeting

Language: Английский

Citations

2
Cancer-associated fibroblasts: Key criminals of tumor pre-metastatic niche DOI Creative Commons
Guozhang Dong, Peng Chen,

Youtao Xu

et al.

Cancer Letters, Journal Year: 2023, Volume and Issue: 566, P. 216234 - 216234

Published: May 24, 2023

Cancer-associated fibroblasts (CAFs) are abundant and important components of the tumour mesenchyme, have been extensively studied for their role in primary tumours. CAFs provide biomechanical support cells play key roles immunosuppression metastasis. can promote epithelial-mesenchymal transition (EMT) by secreting extracellular vesicles (EVs), increasing adhesion to cells, remodelling matrix (ECM) tumour, changing its mechanical stiffness, which provides a pathway Moreover, form cell clusters with circulating (CTCs) help them resist blood shear forces achieve colonisation distant host organs. Recent studies revealed pre-metastatic niche (PMN) formation prevention. In this review, we discuss PMN therapeutic interventions targeting prevent

Language: Английский

Citations

35
Engine shutdown: migrastatic strategies and prevention of metastases DOI Creative Commons
Martina Raudenská,

Kateřina Petrláková,

Tamara Juriňáková

et al.

Trends in cancer, Journal Year: 2023, Volume and Issue: 9(4), P. 293 - 308

Published: Feb. 15, 2023

Most cancer-related deaths among patients with solid tumors are caused by metastases. Migrastatic strategies represent a unique therapeutic approach to prevent all forms of cancer cell migration and invasion. Because the machinery has been shown promote metastatic dissemination, successful migrastatic therapy may reduce need for high-dose cytotoxic therapies that currently used risk dissemination. In this review we focus on anti-invasive antimetastatic hold promise treatment tumors. The best targets would be those required motility, such as ATP availability, mitochondrial metabolism, cytoskeletal dynamics contractility.

Language: Английский

Citations

34
Targeting the p53 signaling pathway in cancers: Molecular mechanisms and clinical studies DOI Creative Commons
Jinze Shen,

Qurui Wang,

Yunan Mao

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: May 28, 2023

Tumor suppressor p53 can transcriptionally activate downstream genes in response to stress, and then regulate the cell cycle, DNA repair, metabolism, angiogenesis, apoptosis, other biological responses. has seven functional domains 12 splice isoforms, different subtypes play roles. The activation inactivation of are finely regulated associated with phosphorylation/acetylation modification ubiquitination modification, respectively. Abnormal is closely related occurrence development cancer. While targeted therapy signaling pathway still its early stages only a few drugs or treatments have entered clinical trials, new ongoing trials expected lead widespread use signaling-targeted cancer treatment future. TRIAP1 novel inhibitor apoptosis. homolog yeast mitochondrial intermembrane protein MDM35, which tumor-promoting role by blocking mitochondria-dependent apoptosis pathway. This work provides systematic overview recent basic research progress proposes that an important therapeutic target signaling.

Language: Английский

Citations

26
Blebology: principles of bleb-based migration DOI
Juan Manuel GARCIA ARCOS, Ankita Jha, Clare M. Waterman

et al.

Trends in Cell Biology, Journal Year: 2024, Volume and Issue: 34(10), P. 838 - 853

Published: March 27, 2024

Language: Английский

Citations

16
Immunosurveillance encounters cancer metabolism DOI Creative Commons

Yu‐Ming Chuang,

Sheue‐Fen Tzeng, Ping‐Chih Ho

et al.

EMBO Reports, Journal Year: 2024, Volume and Issue: 25(2), P. 471 - 488

Published: Jan. 12, 2024

Abstract Tumor cells reprogram nutrient acquisition and metabolic pathways to meet their energetic, biosynthetic, redox demands. Similarly, processes in immune support host immunity against cancer determine differentiation fate of leukocytes. Thus, deregulation imbalance within the tumor microenvironment have been reported drive evasion compromise therapeutic outcomes. Interestingly, emerging evidence indicates that anti-tumor could modulate heterogeneity, aggressiveness, reprogramming, suggesting immunosurveillance can instruct progression multiple dimensions. This review summarizes our current understanding how crosstalk tumors affects immunogenicity promotes progression. Furthermore, we explain defects cascade contribute developing dysfunctional responses cancers discuss contribution these as a feedback mechanism. Finally, highlight ongoing clinical trials new strategies targeting cellular metabolism cancer.

Language: Английский

Citations

15
Obesity, Dietary Fats, and Gastrointestinal Cancer Risk-Potential Mechanisms Relating to Lipid Metabolism and Inflammation DOI Creative Commons
Kathleen A. J. Mitchelson, Fiona O’Connell, Jacintha O’Sullivan

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 42 - 42

Published: Jan. 10, 2024

Obesity is a major driving factor in the incidence, progression, and poor treatment response gastrointestinal cancers. Herein, we conducted comprehensive analysis of impact obesity its resulting metabolic perturbations across four cancer types, namely, oesophageal, gastric, liver, colorectal cancer. Importantly, not all obese phenotypes are equal. Obese adipose tissue heterogeneity depends on location, structure, cellular profile (including resident immune cell populations), dietary fatty acid intake. We discuss whether impacts tumorigenic environment. Dietary fat quality, particular saturated acids, promotes hypertrophic, pro-inflammatory profile, contrast to monounsaturated hyperplastic, less inflammatory phenotype. The purpose this review examine obesity, including biology oncogenesis, specifically focusing lipid metabolism mechanisms. This achieved with focus cancers as exemplar models obesity-associated

Language: Английский

Citations

11
Bridging epigenomics and tumor immunometabolism: molecular mechanisms and therapeutic implications DOI Creative Commons
Xiaowen Xie, Weici Liu, Zhiyuan Yuan

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 8, 2025

Epigenomic modifications—such as DNA methylation, histone acetylation, and methylation—and their implications in tumorigenesis, progression, treatment have emerged a pivotal field cancer research. Tumors undergo metabolic reprogramming to sustain proliferation metastasis nutrient-deficient conditions, while suppressing anti-tumor immunity the tumor microenvironment (TME). Concurrently, immune cells within immunosuppressive TME adaptations, leading alterations function. The complicated interplay between metabolites epigenomic modulation has spotlighted significance of regulation immunometabolism. In this review, characteristics modification associated with tumors are systematically summarized alongside regulatory roles Classical emerging approaches delineated broaden boundaries research on crosstalk immunometabolism epigenomics. Furthermore, we discuss potential therapeutic strategies that target modulate modifications, highlighting burgeoning synergy therapies immunotherapy promising avenue for treatment.

Language: Английский

Citations

1
The Caenorhabditis elegans anchor cell transcriptome: ribosome biogenesis drives cell invasion through basement membrane DOI Open Access
Daniel S. Costa, Isabel W. Kenny-Ganzert, Qiuyi Chi

et al.

Development, Journal Year: 2023, Volume and Issue: 150(9)

Published: April 11, 2023

ABSTRACT Cell invasion through basement membrane (BM) barriers is important in development, immune function and cancer progression. As BM often stochastic, capturing gene expression profiles of actively invading cells vivo remains elusive. Using the stereotyped timing Caenorhabditis elegans anchor cell (AC) invasion, we generated an AC transcriptome during breaching. Through a focused RNAi screen transcriptionally enriched genes, identified new regulators, including translationally controlled tumor protein (TCTP). We also discovered enrichment ribosomal proteins. AC-specific RNAi, endogenous ribosome labeling biogenesis analysis revealed that burst production occurs shortly after specification, which drives translation proteins mediating removal. Ribosomes enrich near endoplasmic reticulum (ER) Sec61 translocon endomembrane system expands before invasion. show sensitive to ER stress, indicating heightened requirement for ER-trafficked These studies reveal key roles expansion establish as resource identify mechanisms underlying transmigration.

Language: Английский

Citations

20