PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(3), P. e0320287 - e0320287
Published: March 31, 2025
Image-based
cell
phenotyping
is
fundamental
in
both
biology
and
medicine.
As
cells
are
dynamic
systems,
based
on
static
data
complemented
by
extracted
from
time-dependent
characteristics.
We
developed
a
label-free
automatic
tracking
method
for
phase
contrast
images.
examined
the
possibility
of
using
motility-based
discrimination
to
identify
different
types
mesenchymal
migration
invasive
malignant
cancer
non-cancer
cells.
These
were
cultured
plastic
tissue
culture
vessels,
motility
parameters
trajectories
with
tracking.
Correlation
analysis
these
identified
characteristic
HT1080
fibrosarcoma
3T3-Swiss
fibroblast
lines.
The
parameter
“sum
turn
angles,”
combined
“frequency
turns”
at
shallow
angles
“migration
speed,”
proved
effective
highlighting
characteristics
It
revealed
differences
their
mechanisms
generating
propulsive
forces.
requirements
characterize
included
spatiotemporal
resolution
segmentation
tracking,
capable
detecting
polarity
changes
associated
morphological
alterations
body
displacement.
With
proposed
here,
curve
computed
quadratic
angles”
turns
below
30°”
gave
best
performance
94%
sensitivity.
Cell
process
related
not
only
but
also
healing
growth.
methodology
easy
use,
enabling
anyone
without
professional
skills
image
analysis,
large
training
datasets,
or
special
devices.
has
potential
application
broad
range
applications
basic
research.
Validating
expandability
this
migration,
including
scheme
force
generation,
an
important
consideration
future
study.
Free Radical Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 14
Published: Jan. 20, 2025
PurposeThe
concept
of
dual-state
hyper-energy
metabolism
characterized
by
elevated
glycolysis
and
OxPhos
has
gained
considerable
attention
during
tumor
growth
metastasis
in
different
malignancies.
However,
it
is
largely
unknown
how
such
metabolic
phenotypes
influence
the
radiation
response
aggressive
cancers.
Therefore,
present
study
aimed
to
investigate
impact
(increased
OxPhos)
on
a
human
glioma
cell
line.MethodsModulation
mitochondrial
electron
transport
chain
was
carried
out
using
2,4-dinitrophenol
(DNP).
Metabolic
characterization
assessing
glucose
uptake,
lactate
production,
mass,
membrane
potential,
ATP
production.
The
examined
growth,
clonogenic
survival,
death
assays.
Macromolecular
oxidation
assessed
DNA
damage,
lipid
peroxidation,
protein
carbonylation
assay.ResultsHypermetabolic
OPM-BMG
cells
exhibited
significant
increase
following
irradiation
as
compared
parental
BMG-1
cells.
Enhanced
radioresistance
evidenced
α/β
ratio
(9.58)
D1
dose
(4.18
Gy)
4.36
2.19
Gy
respectively.
Moreover,
were
found
exhibit
increased
resistance
against
radiation-induced
death,
macromolecular
Inhibition
complex-II
significantly
enhanced
radiosensitivity
cells.ConclusionOur
results
demonstrate
that
confer
radioresistance.
Consequently
targeting
combination
with
may
overcome
therapeutic
cancers
like
glioma.
Cancer Drug Resistance,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 26, 2025
Cancer
cells
often
develop
tolerance
to
chemotherapy,
targeted
therapy,
and
immunotherapy
drugs
either
before
or
during
treatment.
The
significant
heterogeneity
among
various
tumors
poses
a
critical
challenge
in
modern
cancer
research,
particularly
overcoming
drug
resistance.
Copper,
as
an
essential
trace
element
the
body,
participates
biological
processes
of
diseases,
including
cancers.
growth
many
types
tumor
exhibits
heightened
dependence
on
copper.
Thus,
targeting
copper
metabolism
inducing
cuproptosis
may
be
potential
ways
overcome
Copper
chelators
have
shown
resistance
by
copper-dependent
cells.
In
contrast,
ionophores,
copper-based
nanomaterials,
other
small
molecules
been
used
induce
cell
death
(cuproptosis)
cells,
drug-resistant
This
review
summarizes
regulation
role
resistance,
providing
ideas
for
future.
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(3), P. e0320287 - e0320287
Published: March 31, 2025
Image-based
cell
phenotyping
is
fundamental
in
both
biology
and
medicine.
As
cells
are
dynamic
systems,
based
on
static
data
complemented
by
extracted
from
time-dependent
characteristics.
We
developed
a
label-free
automatic
tracking
method
for
phase
contrast
images.
examined
the
possibility
of
using
motility-based
discrimination
to
identify
different
types
mesenchymal
migration
invasive
malignant
cancer
non-cancer
cells.
These
were
cultured
plastic
tissue
culture
vessels,
motility
parameters
trajectories
with
tracking.
Correlation
analysis
these
identified
characteristic
HT1080
fibrosarcoma
3T3-Swiss
fibroblast
lines.
The
parameter
“sum
turn
angles,”
combined
“frequency
turns”
at
shallow
angles
“migration
speed,”
proved
effective
highlighting
characteristics
It
revealed
differences
their
mechanisms
generating
propulsive
forces.
requirements
characterize
included
spatiotemporal
resolution
segmentation
tracking,
capable
detecting
polarity
changes
associated
morphological
alterations
body
displacement.
With
proposed
here,
curve
computed
quadratic
angles”
turns
below
30°”
gave
best
performance
94%
sensitivity.
Cell
process
related
not
only
but
also
healing
growth.
methodology
easy
use,
enabling
anyone
without
professional
skills
image
analysis,
large
training
datasets,
or
special
devices.
has
potential
application
broad
range
applications
basic
research.
Validating
expandability
this
migration,
including
scheme
force
generation,
an
important
consideration
future
study.
