AuNPs/CNC Nanocomposite with A “Dual Dispersion” Effect for LDI‐TOF MS Analysis of Intact Proteins in NSCLC Serum Exosomes DOI Creative Commons
Liang Shan, Yongxia Qiao,

Lifang Ma

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(12)

Published: Jan. 15, 2024

Abstract Detecting exosomal markers using laser desorption/ionization time‐of‐flight mass spectrometry (LDI‐TOF MS) is a novel approach for examining liquid biopsies of non‐small cell lung cancer (NSCLC) samples. However, LDI‐TOF MS limited by low sensitivity and poor reproducibility when analyzing intact proteins directly. In this report, gold nanoparticles/cellulose nanocrystals (AuNPs/CNC) introduced as the matrix direct analysis in NSCLC serum exosomes. AuNPs/CNC with “dual dispersion” effects dispersed stabilized AuNPs improved ion inhibition caused protein aggregation. These features increased signal‐to‐noise ratio [M+H] + peaks two orders magnitude lowered detection limit to 0.01 mg mL –1 . The coefficient variation or without measured 10.2% 32.5%, respectively. excellent yielded linear relationship ( y = 15.41 x – 7.983, R 2 0.989) over concentration range 20 Finally, AuNPs/CNC‐assisted provides clinically relevant fingerprint information serum, characteristic S100 calcium‐binding A10, Urokinase plasminogen activator surface receptor, Plasma protease C1 inhibitor, Tyrosine‐protein kinase Fgr Mannose‐binding lectin associated serine represented predictive biomarkers risk.

Language: Английский

Mitigation of Sepsis-Induced Acute Lung Injury by BMSC-Derived Exosomal miR-125b-5p Through STAT3-Mediated Suppression of Macrophage Pyroptosis DOI Creative Commons

Yiming Tao,

Xinxin Xu, Bin Yang

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 7095 - 7113

Published: Nov. 1, 2023

Sepsis is a syndrome characterized by high morbidity and mortality rates. One of its most severe complications acute lung injury, which exhibits multitude clinical biological features, including macrophage pyroptosis. This study investigates the regulatory effects exosomes derived from Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) on sepsis-associated injury (ALI) explores potential mechanisms mediated exosomal miRNAs.Exosomes were isolated primary BMSCs adult C57BL/6J mice using differential centrifugation. Their uptake distribution in both vitro vivo contexts validated. Key hub gene signal transducer activator transcription 3 (STAT3) upstream non-coding miR-125b-5p elucidated through combination bioinformatics, machine learning, miRNA sequencing. Subsequently, therapeutic BMSC-derived alleviating sepsis-induced was substantiated. Moreover, functionalities STAT3 corroborated inhibitor agonist interventions, employing gain loss-of-function strategies vivo. Finally, dual-luciferase reporter assay reaffirmed interaction between STAT3.We confirmed their accumulation mouse as well macrophages vitro. identified pivotal demonstrated that can target STAT3, thereby inhibiting MiR-125b-5p inhibition experiments showed mitigate pyroptosis delivering miR-125b-5p. overexpression validated reduces suppressing STAT3. Furthermore, binding STAT3.Exosomes BMSCs, serving carriers for miR-125b-5p, downregulate ALI. These significant findings provide valuable insights into development ALI therapies centred around BMSC.

Language: Английский

Citations

17
Standardized Reporting of Research on Exosomes to Ensure Rigor and Reproducibility DOI
Anita Yadav, Yi Xuan, Chandan K. Sen

et al.

Advances in Wound Care, Journal Year: 2024, Volume and Issue: 13(11), P. 584 - 599

Published: June 18, 2024

Significance: The study of extracellular vesicles (EV), especially exosomes, has unlocked new avenues in understanding cellular communication and potential therapeutic avenues. Recent Advances: Advancements EV research have shown significant contributions from the International Society for Extracellular Vesicles (ISEV), establishing methodological standards. evolution MISEV guidelines 2014 to 2023 reflects enhanced rigor reproducibility. launch EV-TRACK platform promotes uniformity reproducibility by providing a centralized repository data sharing standardization practices. Furthermore, databases like EVpedia ExoCarta facilitated collaboration within scientific community. Concurrently, exosome-based therapies emerged as forefront area regenerative medicine targeted drug delivery, showcasing exosomes promoting tissue regeneration. Critical Issues: Despite advancements, field grapples with challenges such vesicular heterogeneity, isolation complexity, standardization. These issues impact clinical applications. inconsistency exosomal preparations trials poses efficacy safety. Future Directions: review outlines critical areas future research, including need technological innovation characterization, establishment standardized protocols, deeper exosome biology. also highlights reassess guidelines, develop characterization technologies, establish protocols overcome current limitations. Emphasis is placed on interdisciplinary address complexities biology, improve trial design, ultimately realize exosome's diagnostic potential. Continued evaluation rigorous validation are essential successful integration.

Language: Английский

Citations

8
Application of exosomes in tumor immunity: recent progresses DOI Creative Commons
Haiyan Qiu,

Junting Liang,

Guang Yang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: April 3, 2024

Exosomes are small extracellular vesicles secreted by cells, ranging in size from 30 to 150 nm. They contain proteins, nucleic acids, lipids, and other bioactive molecules, which play a crucial role intercellular communication material transfer. In tumor immunity, exosomes present various functions while the following two of great importance: regulating immune response serving as delivery carriers. This review starts with introduction formation, compositions, functions, isolation, characterization, applications exosomes, subsequently discusses current status immunotherapy, recent exosome-based immunity regulation antitumor drug delivery. Finally, challenge future prospects proposed hope demonstrate inspiration for targeted readers field.

Language: Английский

Citations

7
Hypoxia-Driven Changes in Tumor Microenvironment: Insights into Exosome-Mediated Cell Interactions DOI Creative Commons

Churan Wang,

Shun Xu,

Xiao Yang

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 8211 - 8236

Published: Aug. 1, 2024

Hypoxia, as a prominent feature of the tumor microenvironment, has profound impact on multicomponent changes within this environment. Under hypoxic conditions, malignant phenotype cells, variety cell types well intercellular communication and material exchange, undergo complex alterations. These provide significant prospects for exploring mechanisms development under different microenvironmental conditions devising therapeutic strategies. Exosomes secreted by cells stromal are integral components serving crucial mediators have consequently garnered increasing attention from researchers. This review focuses which promote release exosomes alter their encapsulated contents. It also examines effects derived immune other microenvironment. Additionally, we summarize current research progress potential clinical applications propose future directions in field.

Language: Английский

Citations

7
AuNPs/CNC Nanocomposite with A “Dual Dispersion” Effect for LDI‐TOF MS Analysis of Intact Proteins in NSCLC Serum Exosomes DOI Creative Commons
Liang Shan, Yongxia Qiao,

Lifang Ma

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(12)

Published: Jan. 15, 2024

Abstract Detecting exosomal markers using laser desorption/ionization time‐of‐flight mass spectrometry (LDI‐TOF MS) is a novel approach for examining liquid biopsies of non‐small cell lung cancer (NSCLC) samples. However, LDI‐TOF MS limited by low sensitivity and poor reproducibility when analyzing intact proteins directly. In this report, gold nanoparticles/cellulose nanocrystals (AuNPs/CNC) introduced as the matrix direct analysis in NSCLC serum exosomes. AuNPs/CNC with “dual dispersion” effects dispersed stabilized AuNPs improved ion inhibition caused protein aggregation. These features increased signal‐to‐noise ratio [M+H] + peaks two orders magnitude lowered detection limit to 0.01 mg mL –1 . The coefficient variation or without measured 10.2% 32.5%, respectively. excellent yielded linear relationship ( y = 15.41 x – 7.983, R 2 0.989) over concentration range 20 Finally, AuNPs/CNC‐assisted provides clinically relevant fingerprint information serum, characteristic S100 calcium‐binding A10, Urokinase plasminogen activator surface receptor, Plasma protease C1 inhibitor, Tyrosine‐protein kinase Fgr Mannose‐binding lectin associated serine represented predictive biomarkers risk.

Language: Английский

Citations

6