Seminal Plasma: Relevant for Fertility?

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(9), P. 4368 - 4368

Published: April 22, 2021

Seminal plasma (SP), the non-cellular component of semen, is a heterogeneous composite fluid built by secretions testis, epididymis and accessory sexual glands. Its composition, despite species-specific anatomical peculiarities, consistently contains inorganic ions, specific hormones, proteins peptides, including cytokines enzymes, cholesterol, DNA RNA-the latter often protected within epididymis- or prostate-derived extracellular vesicles. It beyond question that SP participates in diverse aspects sperm function pre-fertilization events. The also interacts with various compartments tubular genital tract, triggering changes gene prepares for an eventual successful pregnancy; thus, it ultimately modulates fertility. Despite these concepts, imperative to remember SP-free spermatozoa (epididymal washed ejaculated) are still fertile, so this review shall focus on differences between vivo roles following semen deposition female those regarding additions handled artificial reproduction, cryopreservation, from insemination vitro fertilization. This attempts, our own results model animal species, critically summarize current knowledge reproductive played components, particularly which increasingly affected infertility. ultimate goal reconcile delicate balance molecular concentration their concerted effects after temporal exposure vivo. We aim appraise functions relevance as diagnostic biomarkers value additives refine strategies, biotechnologies, livestock models humans.

Language: Английский

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Seminal exosomes – An important biological marker for various disorders and syndrome in human reproduction

Saudi Journal of Biological Sciences, Journal Year: 2021, Volume and Issue: 28(6), P. 3607 - 3615

Published: March 18, 2021

Exosomes are nano-sized membrane vesicles, secreted by different types of cells into the body's biological fluids. They found in abundance semen as compared to other contain a cargo lipid molecules, proteins, phospholipids, cholesterol, mRNAs, and miRNAs. Each molecule seminal exosomes (SE) has potential role male reproduction for childbirth. Many candidates available within that can be used diagnostic markers various diseases or syndromes associated with reproduction. Also these exospmes play major female reproductive tract effective fertilization.

Language: Английский

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Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 7

Published: Jan. 13, 2020

Sperm cryopreservation is an important technique for fertility management, but post-thaw viability of sperm differs among breeding bulls. With metabolites being the end products various metabolic pathways, contributions seminal plasma to are still unknown. These gaps in knowledge base concerning because they prevent advances fundamental science cryobiology and improvement bull fertility. The objective this study was test hypothesis that amino acids associated with freezability sperm. To accomplish objective, acid concentrations from seven bulls good (GF) six poor (PF) were quantified using gas chromatography-mass spectrometry (GC-MS). Multivariate univariate analyses performed identify potential biomarkers. Pathways networks identified bioinformatic tools. By analyzing interpreting results we demonstrated glutamic most abundant average concentration 3,366 ± 547.3 nM, which accounts about 53% total acids. other predominant alanine, glycine, aspartic mean 1,053 187.9, 429.8 57.94, 427 101.3 nM. Pearson's correlation analysis suggested phenylalanine significantly (r = 0.57, P-value 0.043). Significant correlations also found In addition, partial least squares-discriminant (PLS-DA) bi-plot indicated a distinct separation between GF PF groups. Phenylalanine had highest VIP score more groups than Moreover, pathway network contributes oxidoreductase antioxidant reactions. Although did not yield significant differences two groups, these findings indicate potentially roles plasma, thereby building foundation reproductive biotechnology.

Language: Английский

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Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance

Antioxidants, Journal Year: 2019, Volume and Issue: 8(11), P. 539 - 539

Published: Nov. 9, 2019

While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains main source antioxidant enzymes able scavenge these reactive oxygen species. Therefore, stallion components may have an impact on ejaculate freezability. Against background, study was designed investigate whether activities are related cryotolerance. With purpose, 16 ejaculates were collected from 14 healthy stallions, and each split into two aliquots. The first one used evaluate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), reductase (GSR) in plasma. second aliquot extended then processed for cryopreservation. Sperm motility viability evaluated before after classified as good (GFE) or poor freezability (PFE) based total motile viable spermatozoa at post-thaw. We observed that, while specific CAT, GPX, GSR similar between GFE PFE, that SOD significantly (p < 0.05) higher than PFE. can thus conclude activity given might be its

Language: Английский

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Antioxidants and their effect on the oxidative/nitrosative stress of frozen-thawed boar sperm

Cryobiology, Journal Year: 2020, Volume and Issue: 98, P. 5 - 11

Published: Nov. 25, 2020

Language: Английский

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Reproductive physiology of the boar: What defines the potential fertility of an ejaculate?

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 269, P. 107476 - 107476

Published: April 18, 2024

Despite decades of research and handling semen for use in artificial insemination (AI) other assisted reproductive technologies, 5-10% selected boar sires are still considered sub-fertile, escaping current assessment methods sperm quality resilience to preservation. As end-product, the ejaculate (emitted spermatozoa sequentially exposed composite seminal plasma, SP) ought define homeostasis testes, epididymis, accessory sexual glands. Yet, linking findings production biology fertility is suboptimal. The present essay critically reviews how a fertile can help us diagnose both health handling, focusing on -available under development- identify suitable biomarkers cryotolerance fertility. Bulk SP, proteins microRNAs (miRNAs) have, albeit linked function after AI, failed enhance outcomes at commercial level, perhaps just being components complex functional pathway. Hence, focus now interaction sperm-SP, comparing vivo with ex vivo, regarding nano-sized lipid bilayer extracellular vesicles (sEVs) as priority. sEVs transport fragile molecules (lipids, proteins, nucleic acids) which, shielded from degradation, mediate cell-to-cell communication female internal genital tract. Such modulates essential processes, immunological tolerance. be harvested, characterized, stored, manipulated, e.g. used andrological diagnosis, selection breeders, alternatively additives improve cryosurvival

Language: Английский

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Boar-to-Boar Variations in Quality Characteristics of Sperm from Different Ejaculates Following Freezing–Thawing

Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 212 - 212

Published: Feb. 2, 2025

The main objective of this study was to investigate boar-to-boar variations in the quality characteristics sperm from sperm-rich fractions (SRFs) and whole ejaculates (WEs) following freezing–thawing. Several attributes, such as motility patterns analyzed by computer-assisted analysis (CASA) system, mitochondrial function, membrane integrity, DNA fragmentation were used compare cryo-survival SRFs WEs boars with good poor semen freezability (GSF PSF, respectively). In study, post-thaw total (TMOT) more than 30% (>30%) classified having GSF, while those TMOT less (<30%) PSF. Principal component 1 (PCA1), which is sample variation, explained approximately 75% variance between GSF PSF groups, reaffirming reliability a reliable criterion classify animals. Most parameters positively correlated. Furthermore, scatter plot analyses show stronger relationships frozen–thawed (FT) WEs. Individual boar or source had marked effects on FT sperm. higher TMOT, velocity straight line (VSL), average path (VAP) enhanced compared group. markedly WEs, particularly for boars. We suggest that potential group does not differ significantly variability an important factor affects

Language: Английский

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Total and specific activities of superoxide dismutase (SOD) in seminal plasma are related with the cryotolerance of jackass spermatozoa

Cryobiology, Journal Year: 2019, Volume and Issue: 92, P. 109 - 116

Published: Nov. 26, 2019

Language: Английский

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Exosomes in specific fractions of the boar ejaculate contain CD44: A marker for epididymosomes?

Theriogenology, Journal Year: 2019, Volume and Issue: 140, P. 143 - 152

Published: Aug. 21, 2019

Language: Английский

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Aquaporins and (in)fertility: More than just water transport

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2020, Volume and Issue: 1867(3), P. 166039 - 166039

Published: Dec. 17, 2020

Language: Английский

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