Journal of Veterinary Medical Science,
Journal Year:
2021,
Volume and Issue:
83(6), P. 973 - 980
Published: Jan. 1, 2021
Antioxidants
have
multiple
protective
roles
in
a
variety
of
cells
and
thus
can
be
used
to
protect
sperm
against
cryo-damage
during
freezing,
which
affects
fertility.
The
antioxidant
resveratrol
(3,5,4-trihydroxytrans-stilbene;
RSV)
has
been
reported
the
animal
cryopreservation,
including
human
sperm.
In
this
study,
we
assessed
effects
RSV
supplementation
on
dog
cryopreservation.
Semen
was
collected
from
four
dogs
effect
different
concentrations
(0,
100,
200,
400
µM)
post-thaw
quality
examined.
After
thawing,
motility
using
computer-aided
analysis,
structural
integrity
plasma
membrane,
acrosome,
chromatin
addition,
their
mitochondrial
activity
gene
expression
were
also
assessed.
Dog
cryopreserved
with
200
µM
showed
significant
improvement
viability
compared
that
control
group
(P<0.05).
Moreover,
RSV-supplemented
samples
significantly
higher
numbers
an
intact
active
mitochondria,
acrosomes
than
Furthermore,
supplemented
lower
pro-apoptotic
(BAX),
reactive
oxygen
species
(ROS)
modulator
oxidative
stress-related
(ROMO1)
8-oxoguanine
DNA
glycosylase
1
(OGG1)
whereas
levels
anti‐apoptotic
(BCL2),
protamine-2
(PRM2),
protamine-3
(PRM3)
acrosome‐associated
3
(SPACA3)
genes
control.
Our
results
suggest
RSV,
at
its
optimum
concentration,
efficiently
as
cryopreservation
Molecules,
Journal Year:
2020,
Volume and Issue:
25(19), P. 4554 - 4554
Published: Oct. 5, 2020
Resveratrol
is
one
of
the
most
investigated
natural
polyphenolic
compounds
and
contained
in
more
than
70
types
plants
red
wine.
The
widespread
interest
this
polyphenol
derives
from
its
antioxidant,
anti-inflammatory
anti-aging
properties.
Several
studies
have
established
that
resveratrol
regulates
animal
reproduction.
However,
mechanisms
action
potential
therapeutic
effects
are
still
unclear.
This
review
aims
to
clarify
role
male
female
reproductive
functions,
with
a
focus
on
animals
veterinary
interest.
In
females,
has
been
considered
as
phytoestrogen
due
capacity
modulate
ovarian
function
steroidogenesis
via
sirtuins,
SIRT1
particular.
also
used
enhance
aged
oocyte
quality
gametes
cryo-protectant
mainly
antioxidant
anti-apoptotic
effects.
males,
enhances
testes
spermatogenesis
through
activation
AMPK
pathway.
Furthermore,
supplemented
semen
extenders,
improving
preservation
sperm
quality.
conclusion,
potentially
beneficial
for
ameliorating
function.
Antioxidants,
Journal Year:
2020,
Volume and Issue:
9(10), P. 1023 - 1023
Published: Oct. 21, 2020
Reproduction
is
a
complex
process
that
substantially
affected
by
environmental
cues,
specifically
feed/diet
and
its
components.
Farm
animals
as
herbivorous
are
exposed
to
large
amount
of
polyphenols
present
in
their
natural
feeding
system,
alternative
feed
resources
(shrubs,
trees,
agro-industrial
byproducts),
polyphenol-enriched
additives.
Such
exposure
has
increased
because
the
well-known
antioxidant
properties
polyphenols.
However,
date,
argumentation
around
impacts
on
reproductive
events
debatable.
Accordingly,
intensive
inclusion
diets
breeding
media
for
assisted
techniques
needs
further
investigation,
avoiding
any
source
waste
achieving
maximum
benefits.
This
review
illustrates
recent
findings
connecting
dietary
consumption
from
different
sources
(conventional
unconventional
feeds)
with
performance
farm
animals,
underpinned
vitro
studies
this
field.
update
will
help
formulating
proper
diets,
optimizing
introduction
new
plant
species,
additives
improving
function,
possible
wastes
maximizing
Animals,
Journal Year:
2023,
Volume and Issue:
13(18), P. 2829 - 2829
Published: Sept. 6, 2023
This
study
aimed
to
determine
the
effect
of
resveratrol
and
its
optimal
concentration
on
quality
frozen-thawed
(FT)
boar
sperm.
Semen
ejaculates
were
obtained
from
13
Duroc
boars
aged
between
1.5
3
years.
The
sperm
sample
was
separated
into
7
groups
based
concentrations
in
freezing
extender,
which
0
(control),
25,
50,
75,
100,
125,
250
µM,
respectively.
frozen
using
liquid
nitrogen
vapor
thawed
at
50
°C
for
12
s.
After
thawing,
total
motility,
progressive
viability,
intact
acrosomes,
mitochondrial
membrane
potential
level
MDA
assessed.
supplementation
50-100
µM
improved
motility
viability
FT
comparison
control
group
(p
<
0.05).
Furthermore,
significantly
more
protective
than
terms
acrosome,
potential,
Nonetheless,
detrimental
found
a
µM.
In
conclusion,
addition
extender
is
enhancing
cryopreserved
Animals,
Journal Year:
2023,
Volume and Issue:
13(24), P. 3887 - 3887
Published: Dec. 18, 2023
Cryopreservation
generates
a
substantial
quantity
of
ROS
in
semen,
leading
to
decline
sperm
quality
and
fertilization
capacity.
The
objective
this
study
was
investigate
the
effects
resveratrol
its
optimal
concentration
on
ram
after
cryopreservation.
Ram
semen
diluted
with
freezing
medium
containing
different
concentrations
(0,
25,
50,
75,
100
μM).
After
thawing,
various
parameters
such
as
total
motility,
progressive
acrosome
integrity,
plasma
membrane
mitochondrial
potential,
glutathione
(GSH)
content,
synthase
(GPx)
activity,
superoxide
dismutase
(SOD)
catalase
(CAT)
lipid
peroxidation
(LPO)
malondialdehyde
(MDA)
level,
SIRT1
DNA
oxidative
damage,
AMPK
phosphorylation
level
were
assessed.
In
addition,
post-thaw
apoptosis
evaluated.
Comparatively,
addition
up
75
μM
significantly
improved
motility
cryopreserved
sperm.
Specifically,
50
demonstrated
notable
enhancement
antioxidant
capacity,
adenosine
triphosphate
(ATP)
levels
compared
control
group
(p
<
0.05).
It
also
0.05)
reduced
damage
DNA.
However,
detrimental
observed
at
resveratrol.
conclusion,
cryopreservation
solution
is
for
enhancing
Reproduction in Domestic Animals,
Journal Year:
2024,
Volume and Issue:
59(1)
Published: Jan. 1, 2024
Abstract
The
use
of
antioxidants
for
semen
preservation
prevents
oxidative
damage
caused
by
reactive
oxygen
species
(ROS).
One
the
most
promising
natural
is
resveratrol,
a
phytoalexin
derived
from
plants,
grapes,
berries,
peanuts
and
red
wine.
To
evaluate
effect
resveratrol
on
quality
redox
status
cryopreserved
bovine
semen.
Five
bulls
were
subjected
to
electroejaculation
obtain
15
ejaculates.
Each
ejaculate
was
extended
with
tris‐egg
yolk‐glycerol‐based
medium
divided
into
six
aliquots
supplemented
0
(control),
10,
20,
30,
40
50
μM
resveratrol.
Semen
frozen
liquid
nitrogen
vapours.
Post‐thawing,
motility
kinetics
evaluated
using
computer‐assisted
sperm
analysis
(CASA)
system,
membrane
integrity
hypoosmotic
test
(HOST),
morphology
staining
eosin–nigrosin,
vitality
fluorescence
microscopy
SYBR14/IP
probes.
Total
antioxidant
capacity
(TAC)
ABTS
•+
assay
ROS
spectrofluorimetry
H
2
DCFDA
probe.
For
statistical
linear
models
adjusted
means
compared
Tukey
test.
All
concentrations
reduced
post‐thawed
sperm.
Supplementation
kinetics,
between
30
alterations
in
observed.
However,
at
increased
TAC
20
μM,
it
production
Resveratrol
appears
have
dose‐dependent
which
higher
doses
produce
greater
alterations,
however,
can
increase
during
freezing.
It
concluded
that
reduce
its
10
reduces
post‐thawing
quality.
IRAQI JOURNAL OF AGRICULTURAL SCIENCES,
Journal Year:
2023,
Volume and Issue:
54(4), P. 1074 - 1083
Published: Aug. 29, 2023
This
study
was
conducted
to
explain
the
effect
of
adding
different
resveratrol
(Res)
concentrations
soybean-lecithin
extender
on
some
post-cryopreserved
(PC)
semen
characteristics
Iraqi
buffalo
bulls
for
preservation
periods.
Eight
mature
were
used,
and
polled
equally
divided
into
four
groups
within
one
trial.
Three
Res
(100,
150,
200
μM)
added
extender,
comparisons
in
response
made
with
control
group
(soybean-lecithin
Res-0).
The
differences
among
sperm
cell
individual
motility
percentages
at
periods
lacked
significance.
Greater
(P≤0.01)
acrosome
integrity
percentage
noticed
Res-150
compared
C
Res-200
cooling,
48
hrs.,
2
months
PC.
Lesser
malondialdehyde
DNA
damage
observed
as
other
groups,
Higher
total
antioxidant
activity
shown
Res-100
In
conclusion,
enhanced
PC
Journal of King Saud University - Science,
Journal Year:
2020,
Volume and Issue:
33(1), P. 101226 - 101226
Published: Nov. 4, 2020
Despite
the
widespread
use
of
frozen
or
refrigerated
mammalian
spermatozoa,
their
quality
remains
low
as
they
are
highly
sensitivity
to
injuries
during
preservation
and
thawing.
Moreover,
in
vitro
conditions
spermatozoa
storage
may
affect
sperm
thereby
oocyte
fertilization,
cleavage,
blastocyst
development.
Recently,
antioxidants
have
been
employed
semen
extenders
for
livestock
different
protocols
compensate
depletion
endogenous
antioxidant
concentration
seminal
plasma
due
dilution
well
counteract
oxidative
stress
minimize
free
radical
generation.
The
present
article
reviews
most
effective
enzymatic
non-enzymatic
used
preservation.
