Data in Brief,
Journal Year:
2022,
Volume and Issue:
45, P. 108578 - 108578
Published: Sept. 6, 2022
This
paper
provides
a
detailed
set
of
data
on
how
the
stallion
sperm
proteome
differs
among
stallions
with
different
motilities,
although
within
normal
ranges.
Findings
distinguish
proteins
that
may
help
to
identify
superior
motility.
Sperm
were
analyzed
using
UHPLC/MS/MS
system
comprising
an
Agilent
1290
infinity
series
UHPLC
coupled
6550
Q-TOF
mass
spectrometer
(Agilent
Technologies,
Santa
Clara,
CA,
USA).
These
can
be
used
disclose
potential
targets
good
samples
and
study
specific
pathways
involved
in
regulation
article
is
linked
"Proteins
mitochondrial
metabolic
functions
fertilization
predominate
better
motility
Journal
Proteomics
247:104335
doi:
10.1016/j.jprot.2021.104335".
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(11), P. 5920 - 5920
Published: May 25, 2022
Flow
cytometry
becomes
a
common
method
for
analysis
of
spermatozoa
quality.
Standard
sperm
characteristics
such
as
viability,
acrosome
and
chromatin
integrity,
oxidative
damage
(ROS)
etc.
can
be
easily
assess
in
any
animal
semen
samples.
Moreover,
several
fertility-related
markers
were
observed
humans
some
other
mammals.
However,
these
fertility
biomarkers
have
not
been
previously
studied
ram.
The
aim
this
study
was
to
optimize
the
flow-cytometric
standard
novel
ram
semen.
Ram
samples
from
Slovak
native
sheep
breeds
analyzed
using
CASA
system
motility
concentration
subsequently
stained
with
fluorescent
dyes
or
specific
antibodies
evaluate
viability
(SYBR-14),
apoptosis
(Annexin
V,
YO-PRO-1,
FLICA,
Caspases
3/7),
status
(PNA,
LCA,
GAPDHS),
capacitation
(merocyanine
540,
FLUO-4
AM),
mitochondrial
activity
(MitoTracker
Green,
rhodamine
123,
JC-1),
ROS
(CM-H2DCFDA,
DHE,
MitoSOX
Red,
BODIPY),
(acridine
orange),
leukocyte
content,
ubiquitination
aggresome
formation,
overexpression
negative
(MKRN1,
SPTRX-3,
PAWP,
H3K4me2).
Analyzed
divided
into
two
groups
according
indicators
quality:
Group
1
(viability
over
60%)
2
under
60%).
Significant
(p
<
0.05)
differences
found
between
concentration,
apoptosis,
integrity
(only
PNA),
activity,
production
(except
DHE),
high
PAWP
expression.
In
conclusion,
probes
confirmed
suitable
multiplex
by
flow
well
validated
detection
ubiquitin,
H3K4me2
spermatozoa.
Genes,
Journal Year:
2023,
Volume and Issue:
14(6), P. 1183 - 1183
Published: May 29, 2023
This
study
aimed
to
reveal
the
potential
genetic
basis
for
litter
size,
coat
colour,
black
middorsal
stripe
and
skin
colour
by
combining
genome-wide
association
analysis
(GWAS)
selection
signature
ROH
detection
within
Youzhou
dark
(YZD)
goat
population
(n
=
206)
using
Illumina
GoatSNP54
BeadChip.
In
GWAS,
we
identified
one
SNP
(snp54094-scaffold824-899720)
on
chromosome
11
two
SNPs
26
(snp11508-scaffold142-1990450,
SORCS3)
12
(snp55048-scaffold842-324525,
LOC102187779)
18
(snp56013-scaffold873-22716,
TCF25)
stripe.
contrast,
no
were
colour.
analysis,
295
significant
iHS
genomic
regions
with
a
mean
|iHS|
score
>
2.66,
containing
signatures
encompassing
232
candidate
genes
detected.
particular,
43
GO
terms
KEGG
pathway
significantly
enriched
in
selected
genes,
which
may
contribute
excellent
environmental
adaptability
characteristic
trait
formation
during
domestication
of
YZD
goats.
detection,
4446
segments
282
consensus
regions,
among
nine
common
overlapped
those
detected
method.
Some
known
economic
traits
such
as
reproduction
(TSHR,
ANGPT4,
CENPF,
PIBF1,
DACH1,
DIS3,
CHST1,
COL4A1,
PRKD1
DNMT3B)
development
growth
(TNPO2,
IFT80,
UCP2,
UCP3,
GHRHR,
SIM1,
CCM2L,
CTNNA3
CTNNA1)
revealed
detection.
Overall,
this
is
limited
small
affects
results
GWAS
certain
extent.
Nevertheless,
our
findings
could
provide
first
overview
mechanism
underlying
these
important
novel
insights
into
future
conservation
utilisation
Chinese
germplasm
resources.
PROTEOMICS,
Journal Year:
2024,
Volume and Issue:
24(14)
Published: May 29, 2024
Abstract
The
mammalian
ejaculate
is
very
well
suited
to
proteomics
studies.
As
such,
research
concerning
sperm
offering
a
huge
amount
of
new
information
on
the
biology
spermatozoa.
Among
domestic
animals,
horses
represent
species
special
interest,
in
which
reproductive
technologies
and
sizeable
market
genetic
material
have
grown
exponentially
last
decade.
Studies
using
proteomic
approaches
been
conducted
recent
years,
showing
that
potent
tool
dig
into
stallion
aim
this
review
present
an
overview
conducted,
how
these
studies
improved
our
knowledge
biology.
main
outcomes
so
far
metabolism,
its
importance
functions,
impact
different
proteome,
identification
potential
biomarkers.
Moreover,
seminal
plasma
phosphoproteomics
are
identified
as
areas
major
interest.
Reproduction,
Journal Year:
2022,
Volume and Issue:
164(6), P. F135 - F144
Published: Oct. 18, 2022
The
growing
understanding
of
the
mechanisms
regulating
redox
homeostasis
in
stallion
spermatozoa,
together
with
its
interactions
energetic
metabolism,
is
providing
new
clues
applicable
to
improvement
sperm
conservation
horses.
Based
on
this
knowledge,
extenders,
adapted
biology
are
expected
be
developed
near
future.The
preservation
semen
either
by
refrigeration
or
cryopreservation
a
principal
component
most
animal
breeding
industries.
Although
procedure
has
been
successful
many
species,
others,
substantial
limitations
persist.
In
last
decade,
mechanistic
studies
have
shed
light
molecular
changes
behind
damage
that
spermatozoa
experience
during
preservation.
Most
oxidative,
and
thus
review,
we
aim
provide
an
updated
overview
recent
discoveries
about
how
maintain
homeostasis,
current
procedures
disrupt
regulation
cause
which
affects
viability,
functionality,
fertility
potentially
health
offspring.
We
optimistic
review
will
promote
ideas
for
further
research
improve
technologies,
promoting
translational
wide
scope
applicability
not
only
horses
but
also
other
species
humans.
Authorea (Authorea),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 12, 2024
The
mammalian
ejaculate
is
very
well
suited
for
proteomics
studies.
As
such,
investigations
on
the
sperm
are
offering
a
huge
amount
of
new
information
biology
spermatozoa.
Among
domestic
animals,
horses
represent
special
interest
species,
in
which
reproductive
technologies
and
an
important
market
genetic
material
has
growth
exponentially
last
decade.
Investigations
using
proteomic
approaches
have
been
conducted
recent
years,
showing
that
potent
tool
to
dig
into
stallion
aim
this
review
present
overview
research
conducted,
how
these
studies
improved
our
knowledge
biology.
main
outcomes
so
far
metabolism,
its
importance
functions,
impact
different
proteome,
identification
potential
biomarkers.
Moreover,
seminal
plasma
phosphoproteomics
identified
as
areas
major
interest.
Reproduction Fertility and Development,
Journal Year:
2024,
Volume and Issue:
36(6)
Published: March 11, 2024
We
are
currently
experiencing
a
period
of
rapid
advancement
in
various
areas
science
and
technology.
The
integration
high
throughput
'omics'
techniques
with
advanced
biostatistics,
the
help
artificial
intelligence,
is
significantly
impacting
our
understanding
sperm
biology.
These
advances
will
have
an
appreciable
impact
on
practice
reproductive
medicine
horses.
This
article
provides
brief
overview
recent
field
spermatology
how
they
changing
assessment
quality.
written
from
authors'
perspective,
using
stallion
as
model.
aim
to
portray
changes
occurring
motility
kinematics,
flow
cytometry,
implementation
technologies,
use
intelligence/self-learning
data
analysis.
also
briefly
discuss
some
can
be
readily
available
practitioner,
through
'on-farm'
devices
telemedicine.
Clinical Theriogenology,
Journal Year:
2024,
Volume and Issue:
16
Published: Dec. 13, 2024
Semen
cryopreservation
remains
the
only
viable
method
for
long-term
stallion
sperm
preservation.
Increased
demand
frozen/thawed
in
Assisted
Reproductive
Technologies
(ARTs)
continues
to
result
a
considerable
body
of
research
determining
effects
on
and
clinical
interpretation
quality
semen.
Some
concepts
regarding
impacts
or
methods
might
limit
widespread
implementation
this
technique
private
practice
settings.
Clinically
relevant
data
cryopreservation,
various
protocols
postthaw
analysis,
utilization
ARTs
are
summarized.
Veterinary Research Communications,
Journal Year:
2023,
Volume and Issue:
48(1), P. 357 - 366
Published: Sept. 14, 2023
Canine
seminal
plasma
is
a
complex
fluid
containing
proteins,
peptides,
enzymes,
hormones
as
well
extracellular
vesicles
that
are
involved
in
many
physiological
and
pathological
processes
including
reproduction.
We
examined
the
expression
of
surface
antigens
Aminopeptidase-N
(CD13)
Dipeptidyl
peptidase
IV
(CD26)
by
flow
cytometry.
For
this
study,
third
fraction
ejaculate,
from
fertile
adult
male
German
Shepherd
dogs,
was
manually
collected
twice,
two
days
apart.
FACS
analyses
revealed
CD13
CD26
co-expressed
on
69.3
±
3.7%
only
2.0
0.5%
express
alone.
On
other
hand,
28.6
3.6%
EVs
Our
results
agree
with
hypothesis
needs
to
be
signal-transducing
molecules,
while
CD13,
can
perform
functions
independently
presence
or
co-expression
CD26.
The
obtained
normal
dogs
could
represent
these
enzymes.
Therefore,
it
would
interesting
carry
out
further
studies
evaluate
biomarker
for
prostate
condition
dogs.
