Danubian Animal Genetic Resources,
Journal Year:
2023,
Volume and Issue:
8(1), P. 7 - 13
Published: Aug. 3, 2023
The
aim
of
the
preliminary
study
was
to
improve
success
cryopreservation
stallion
spermatozoa.
effect
different
equilibration
lengths
and
two
techniques
for
freezing
semen
evaluated,
based
on
motility
viability
parameters.
Fresh
diluted
with
BotuCrio
extender
divided
into
3
groups
equilibrated
0.5h,
2h,
4h.
Subsequently,
half
straws
each
group
were
cryopreserved
in
vapours
liquid
nitrogen
or
automatic
freezer.
Results
spermatozoa
progressive
showed
decreasing
trend
increased
time
equilibration.
Moreover,
a
significant
difference
(p
<
0.05)
percentage
producing
ROS
found
between
frozen
compared
by
freezer
same
time.
Nevertheless,
results
this
are
character.
Experiments
using
higher
number
individuals
need
be
tested
order
find
best
procedure
Slovak
national
horse
breeds.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(4), P. 3379 - 3379
Published: Feb. 8, 2023
For
decades
now,
sperm
cryopreservation
has
been
a
pillar
of
assisted
reproduction
in
animals
as
well
humans.
Nevertheless,
the
success
varies
across
species,
seasons,
and
latitudes
even
within
same
individual.
With
dawn
progressive
analytical
techniques
field
genomics,
proteomics,
metabolomics,
new
options
for
more
accurate
semen
quality
assessment
have
become
available.
This
review
summarizes
currently
available
information
on
specific
molecular
characteristics
spermatozoa
that
could
predict
their
cryotolerance
before
freezing
process.
Understanding
changes
biology
result
exposure
to
low
temperatures
may
contribute
development
implementation
appropriate
measures
assure
high
post-thaw
quality.
Furthermore,
an
early
prediction
or
cryosensitivity
lead
establishment
customized
protocols
interconnecting
adequate
processing
procedures,
techniques,
cryosupplements
are
most
feasible
individual
needs
ejaculate.
Animals,
Journal Year:
2023,
Volume and Issue:
13(14), P. 2300 - 2300
Published: July 13, 2023
The
present
review
aims
to
provide
an
overview
of
the
assay
methods
for
quantification
ROS
and
principal
enzymatic
antioxidants
as
biomarkers
oxidative
stress
in
erythrocytes
spermatozoa
small
domestic
ruminants.
A
complete
literature
search
was
carried
out
PubMed,
Scopus
World
Wide
Web
using
relevant
keywords
focusing
on
last
five
years
(2018-2023).
Among
spectrophotometry,
fluorometry
chemiluminescence,
most
widely
used
method
is
fluorometry,
probably
because
it
allows
simultaneously
several
ROS,
different
probes,
with
greater
economic
advantages.
Regarding
intracellular
antioxidant
enzymes,
recent
reports
only
spectrophotometric
methods,
many
which
use
commercial
kits.
a
less
sensitive
but
cheapest
suitable
both
samples
are
highly
concentrated
ruminant
species.
All
considered
this
have
been
found
be
appropriate;
general,
differences
related
their
costs
sensitivity.
Quantification
activity
may
find
application
study
welfare
health
status
ruminants
monitoring
livestock
production.
Systems Biology in Reproductive Medicine,
Journal Year:
2025,
Volume and Issue:
71(1), P. 90 - 101
Published: March 14, 2025
The
objective
of
this
study
was
to
evaluate
the
effect
a
tannin-rich
extract
from
commercial
Acacia
mearnsii
(MTE_0),
and
gallocatechin,
flavonoid
compound
derived
mearnsii,
on
long-term
viability
motility
cryopreserved
ovine
semen.
Six
fresh
ejaculates
obtained
six
adult
merino
rams
twice
per
week
for
three
weeks
were
allocated
five
aliquots
(0,
12.5,
25,
50,
100
µM
gallocatechin
added
into
Optidyl™
extender)
before
cooling
cryopreservation.
Effects
MTE_0
post-thawed
characteristics
analyzed
using
computer-assisted
semen
analysis
(CASA),
(LIVE/DEAD®
kit,
Molecular
Invitrogen,
Waltham,
MA),
oxidative
stress
(2,7-dichlorodihydrofluorescein
diacetate
(H2DCFDA,
Thermo
Fisher®,
MA))
reactive
oxygen
species
(ROS),
mitochondrial
membrane
potential
(JC-1
MitoTracker,
acrosomal
integrity
(lectin
PNA),
capacitation
merocyanine
540
(M540)
YO-PRO-1
dyes
in
flow
cytometry.
Data
one-way
ANOVA
(IBM
SPSS
21.0
Windows,
Armonk,
NY).
Gallocatechin
at
25
positively
affected
(p
≤
.001)
kinematic
parameters
including
average
path
velocity
(VAP),
progressive
(VSL),
beat
cross
frequency
(BCF)
Similarly,
µM-
improved
sperm
(live
21.99
±
2.06%),
reduced
ROS
levels
(26.45
1.10%),
mitigated
premature
(viable
stable
20.08
1.48%)
compared
other
treatments.
addition
resulted
significant
positive
with
extract.
It
is
concluded
that
inclusion
significantly
reduces
deterioration
following
This
first
introduce
as
an
efficient
antioxidant
additive
improve
its
quality
during
storage.
Our
findings
will
help
post-thaw
longevity.
Future
studies
elucidate
mechanism
anti-oxidative
action
derivatives
longevity
are
recommended.
Theriogenology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 117440 - 117440
Published: April 1, 2025
The
optimization
of
preservation
protocols
(refrigeration
and
freezing)
in
ovine
species
is
necessary
for
a
wider
diffusion
artificial
insemination
this
species.
Besides
the
ram
sperm
quality
assays,
characterization
novel
proteins
could
be
crucial
improving
these
employing
biomarkers.
protein
transmembrane
95
(TMEM95)
membrane
associated
with
oocyte-sperm
fusion
previously
described
bull
or
mouse.
However,
has
not
yet
been
characterized
until
now.
In
work,
different
experimental
groups
based
on
functionality:
capacitated,
refrigerated
at
times
(5
°C
24
h,
5
48
72
h),
frozen-thawed
samples
were
analyzed
compared
to
initial
(15
3
h)
characterize
expression
its
relationship
other
markers
(motility,
kinetic
parameters,
viability,
apoptosis-like
events,
mitochondrial
function,
acrosome-reacted,
zinc
content
as
marker
capacitation).
addition,
capacitation
status
was
tested
by
Fluozin-3,
fluorescent
probe
measuring
used
first
time
sperm.
After
induction,
expected,
acrosome
reactive
spermatozoa
signature
2
significantly
increased,
while
linearity
(P
<
0.05)
decreased
non-capacitated
samples.
Concerning
TMEM95,
profile
increased
after
process,
confirming
status.
Attending
processes,
semen
progressively
during
liquid
storage,
significant
decrease
observed
h
according
fast
progressive
motility
linearity.
TMEM95
showed
same
tendency,
showing
reduction
respect
control
Finally,
cryopreservation
thawed
suffered
detrimental
effect
sample,
concerting
all
studied
parameters
accomplished
When
we
correlation
markers,
highest
positive
correlations
low
capacitated
samples,
such
changes
acrosome-reaction.
On
hand,
between
process
suitable
functionality).
According
our
results,
considered
predictor
early
damage
(cooling
freezing),
considering
integrity
Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(2), P. 65 - 65
Published: Feb. 2, 2024
Artificial
insemination
(AI)
is
used
frequently
in
the
breeding
of
sport
horses,
apart
from
Thoroughbreds.
Most
AIs
are
carried
out
with
cooled
semen
rather
than
frozen
because
difficulties
identifying
a
protocol
that
suitable
for
freezing
most
ejaculates
and
necessity
to
inseminate
close
ovulation
short
life
thawed
spermatozoa.
More
widespread
use
would
improve
biosecurity,
allow
greater
choice
stallions,
offer
more
flexibility
when
managing
deliveries
stud.
It
even
decrease
amount
antibiotics
extenders,
since
volume
smaller
inseminated.
However,
there
considerable
variability
cryosurvival
spermatozoa
different
leading
classification
stallions
as
good
or
bad
freezers.
Improvements
could
be
made
at
level
stallion
nutrition,
collection
regimen,
extender,
removal
seminal
plasma,
cooling
protocol,
among
others.
Stallion
sperm
membranes
highly
susceptible
lipid
peroxidation,
but
research
on
antioxidants
has
failed
identify
an
additive
benefit
all
stallions.
In
future,
biomarkers
freezability
aid
cryopreservation.
