Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics

Cell, Journal Year: 2015, Volume and Issue: 162(3), P. 662 - 674

Published: July 1, 2015

Language: Английский

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Semiconducting Polymer Nanobioconjugates for Targeted Photothermal Activation of Neurons

Journal of the American Chemical Society, Journal Year: 2016, Volume and Issue: 138(29), P. 9049 - 9052

Published: July 12, 2016

Optogenetics provides powerful means for precise control of neuronal activity; however, the requirement transgenesis and incapability to extend neuron excitation window into deep-tissue-penetrating near-infrared (NIR) region partially limit its application. We herein report a potential alternative approach optogenetics using semiconducting polymer nanobioconjugates (SPNsbc) as photothermal nanomodulator thermosensitive ion channels in neurons. SPNsbc are designed efficiently absorb NIR light at 808 nm have conversion efficiency higher than that gold nanorods. By virtue fast heating capability conjunction with targeting channel, can specifically rapidly activate intracellular Ca2+ influx cells reversible safe manner. Our study an organic nanoparticle based strategy eliminates need genetic transfection remotely regulate cellular machinery.

Language: Английский

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An algal photoenzyme converts fatty acids to hydrocarbons

Science, Journal Year: 2017, Volume and Issue: 357(6354), P. 903 - 907

Published: Aug. 31, 2017

Algal enzyme driven by blue light Microalgae make hydrocarbons. In searching for the responsible, Sorigué et al. found a glucose-methanolcholine oxidoreductase (see Perspective Scrutton). Expression of in Escherichia coli showed that hydrocarbon production requires visible light. fact, constant input photons to carry out its catalytic reaction. A long hydrophobic tunnel stabilizes fatty acid substrates proximity flavin adenine dinucleotide cofactor. Science , this issue p. 903 ; see also 872

Language: Английский

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Optochemical Control of Biological Processes in Cells and Animals

Angewandte Chemie International Edition, Journal Year: 2017, Volume and Issue: 57(11), P. 2768 - 2798

Published: May 18, 2017

Biological processes are naturally regulated with high spatial and temporal control, as is perhaps most evident in metazoan embryogenesis. Chemical tools have been extensively utilized cell developmental biology to investigate cellular processes, conditional control methods expanded applications of these technologies toward resolving complex biological questions. Light represents an excellent external trigger since it can be controlled very precision. To this end, several optically developed applied living systems. In review we discuss recent developments optochemical tools, including small molecules, peptides, proteins, nucleic acids that irreversibly or reversibly through light irradiation, a focus on cells animals.

Language: Английский

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Optogenetic Tools for Subcellular Applications in Neuroscience

Neuron, Journal Year: 2017, Volume and Issue: 96(3), P. 572 - 603

Published: Nov. 1, 2017

Language: Английский

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Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth

Nature Communications, Journal Year: 2016, Volume and Issue: 7(1)

Published: Aug. 26, 2016

Abstract Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages light, optogenetics has emerged as an ideal technology this task. Current state-of-the-art methods optical fail combine precision with repeatability cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform robust precise long-term optogenetic regulation protein production in liquid Escherichia coli cultures. Using computer-controlled light-responsive two-component system, accurately track prescribed dynamic green fluorescent profiles through application feedback control, show that system adapts global perturbations such nutrient temperature changes. We demonstrate efficacy potential utility our approach by placing key metabolic enzyme under thus enabling growth rate bacterial physiology studies biotechnology.

Language: Английский

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Environmental effects of ozone depletion and its interactions with climate change: Progress report, 2016

Photochemical & Photobiological Sciences, Journal Year: 2017, Volume and Issue: 16(2), P. 107 - 145

Published: Jan. 26, 2017

Language: Английский

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Mechanisms of Cryptochrome-Mediated Photoresponses in Plants

Annual Review of Plant Biology, Journal Year: 2020, Volume and Issue: 71(1), P. 103 - 129

Published: March 14, 2020

Cryptochromes are blue-light receptors that mediate photoresponses in plants. The genomes of most land plants encode two clades cryptochromes, CRY1 and CRY2, which distinct overlapping within the same species between different plant species. Photoresponsive protein–protein interaction is primary mode signal transduction cryptochromes. exist as physiologically inactive monomers dark; absorption photons leads to conformational change cryptochrome homooligomerization, alters affinity cryptochromes interacting with cryptochrome-interacting proteins form various complexes. These complexes, collectively referred complexome, regulate transcription or stability photoresponsive modulate growth development. activity regulated by photooligomerization; dark monomerization; regulatory proteins; phosphorylation, ubiquitination, degradation. Most more than 30 presently known either other photoreceptors physically interactingwith protein complexes photoreceptors. Some also hormonal signaling proteins. mechanisms enable integrate signals internal external optimize

Language: Английский

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Nanotransducers for Near‐Infrared Photoregulation in Biomedicine

Advanced Materials, Journal Year: 2019, Volume and Issue: 31(33)

Published: June 14, 2019

Abstract Photoregulation, which utilizes light to remotely control biological events, provides a precise way decipher biology and innovate in medicine; however, its potential is limited by the shallow tissue penetration and/or phototoxicity of ultraviolet (UV)/visible that are required match optical responses endogenous photosensitive substances. Thereby, biologically friendly near‐infrared (NIR) with improved desired for photoregulation. Since there few biomolecules absorbing or emitting NIR region, development molecular transducers essential convert into cues regulation events. In this regard, nanomaterials able UV/visible light, heat, free radicals suitable task. Here, recent developments nanotransducers NIR‐light‐mediated photoregulation medicine summarized. The emerging applications, including neural activity, gene expression, visual systems, as well photochemical bonding, highlighted, along design principles nanotransducers. Moreover, current challenges perspectives field discussed.

Language: Английский

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From Conception to Development: Investigating PROTACs Features for Improved Cell Permeability and Successful Protein Degradation

Frontiers in Chemistry, Journal Year: 2021, Volume and Issue: 9

Published: April 20, 2021

Proteolysis Targeting Chimeras (PROTACs) are heterobifunctional degraders that specifically eliminate targeted proteins by hijacking the ubiquitin-proteasome system (UPS). This modality has emerged as an orthogonal approach to use of small-molecule inhibitors for knocking down classic targets and disease-related classified, until now, “undruggable.” In early 2019, first protein reached clinic, drawing attention PROTACs one most appealing technology in drug discovery landscape. Despite these promising results, often affected poor cellular permeability due their high molecular weight (MW) large exposed polar surface area (PSA). Herein, we report a comprehensive record PROTAC design, pharmacology thermodynamic challenges solutions, well some available strategies enhance uptake, including suggestions biological tools vitro evaluation toward successful degradation.

Language: Английский

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