Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: May 7, 2020
En
route
to
a
bio-based
chemical
industry,
the
conversion
of
fatty
acids
into
building
blocks
is
particular
interest.
Enzymatic
routes,
occurring
under
mild
conditions
and
excelling
by
intrinsic
selectivity,
are
particularly
attractive.
Here
we
report
photoenzymatic
cascade
reactions
transform
unsaturated
enantiomerically
pure
secondary
alcohols.
In
first
step
C=C-double
bond
stereoselectively
hydrated
using
oleate
hydratases
from
Lactobacillus
reuteri
or
Stenotrophomonas
maltophilia.
Also,
dihydroxylation
mediated
5,8-diol
synthase
Aspergillus
nidulans
demonstrated.
The
second
comprises
decarboxylation
intermediate
hydroxy
photoactivated
decarboxylase
Chlorella
variabilis
NC64A.
A
broad
range
(poly)unsaturated
can
be
transformed
alcohols
in
simple
one-pot
approach.
ACS Central Science,
Journal Year:
2021,
Volume and Issue:
7(1), P. 55 - 71
Published: Jan. 14, 2021
Biocatalysis,
using
defined
enzymes
for
organic
transformations,
has
become
a
common
tool
in
synthesis,
which
is
also
frequently
applied
industry.
The
generally
high
activity
and
outstanding
stereo-,
regio-,
chemoselectivity
observed
many
biotransformations
are
the
result
of
precise
control
reaction
active
site
biocatalyst.
This
achieved
by
exact
positioning
reagents
relative
to
each
other
fine-tuned
3D
environment,
specific
activating
interactions
between
protein,
subtle
movements
catalyst.
Enzyme
engineering
enables
one
adapt
catalyst
desired
process.
A
well-filled
biocatalytic
toolbox
ready
be
used
various
reactions.
Providing
nonnatural
conditions
evolving
biocatalysts
play
with
myriad
options
creating
novel
transformations
thereby
opening
new,
short
pathways
target
molecules.
Combining
several
pot
perform
reactions
concurrently
increases
efficiency
biocatalysis
even
further.
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
50(14), P. 8003 - 8049
Published: Jan. 1, 2021
Technological
developments
enable
the
discovery
of
novel
enzymes,
advancement
enzyme
cascade
designs
and
pathway
engineering,
moving
biocatalysis
into
an
era
technology
integration,
intelligent
manufacturing
enzymatic
total
synthesis.
Journal of Biological Chemistry,
Journal Year:
2020,
Volume and Issue:
295(3), P. 833 - 849
Published: Jan. 1, 2020
Cytochrome
P450
enzymes
(P450s)
are
broadly
distributed
among
living
organisms
and
play
crucial
roles
in
natural
product
biosynthesis,
degradation
of
xenobiotics,
steroid
drug
metabolism.
P450s
considered
as
the
most
versatile
biocatalysts
nature
because
vast
variety
substrate
structures
types
reactions
they
catalyze.
In
particular,
can
catalyze
regio-
stereoselective
oxidations
nonactivated
C–H
bonds
complex
organic
molecules
under
mild
conditions,
making
useful
production
commodity
pharmaceuticals,
fine
or
bulk
chemicals,
bioremediation
agents,
flavors,
fragrances.
Major
efforts
have
been
made
engineering
improved
systems
that
overcome
inherent
limitations
native
enzymes.
this
review,
we
focus
on
recent
progress
different
strategies,
including
protein
engineering,
redox-partner
electron
source
P450-mediated
metabolic
to
more
efficiently
produce
pharmaceuticals
other
chemicals.
We
also
discuss
future
opportunities
for
applications
systems.
Chemical Society Reviews,
Journal Year:
2020,
Volume and Issue:
49(18), P. 6733 - 6754
Published: Jan. 1, 2020
Small
molecules
such
as
H2,
N2,
CO,
NH3,
O2
are
ubiquitous
stable
species
and
their
activation
role
in
the
formation
of
value-added
products
fundamental
importance
nature
industry.
The
last
few
decades
have
witnessed
significant
advances
chemistry
heavy
low-coordinate
main-group
elements,
with
a
plethora
newly
synthesised
functional
compounds,
behaving
like
transition-metal
complexes
respect
to
facile
small
molecules.
Among
them,
silylenes
received
particular
attention
this
vivid
area
research
showing
even
metal-free
bond
catalysis.
Recent
striking
discoveries
take
advantage
narrow
HOMO-LUMO
energy
gap
Lewis
acid-base
bifunctionality
divalent
Si
centres.
review
is
devoted
recent
using
isolable
corresponding
silylene-metal
for
but
inert
COx,
N2O,
O2,
H2O,
C2H4
E4
(E
=
P,
As).
Chemical Reviews,
Journal Year:
2021,
Volume and Issue:
122(1), P. 1052 - 1126
Published: Nov. 30, 2021
Biocatalysis,
using
enzymes
for
organic
synthesis,
has
emerged
as
powerful
tool
the
synthesis
of
active
pharmaceutical
ingredients
(APIs).
The
first
industrial
biocatalytic
processes
launched
in
half
last
century
exploited
whole-cell
microorganisms
where
specific
enzyme
at
work
was
not
known.
In
meantime,
novel
molecular
biology
methods,
such
efficient
gene
sequencing
and
triggered
breakthroughs
directed
evolution
rapid
development
process-stable
with
broad
substrate
scope
good
selectivities
tailored
substrates.
To
date,
are
employed
to
enable
shorter,
more
efficient,
sustainable
alternative
routes
toward
(established)
small
molecule
APIs,
additionally
used
perform
standard
reactions
API
efficiently.
Herein,
large-scale
synthetic
containing
key
steps
>130
APIs
approved
drugs
drug
candidates
compared
corresponding
chemical
protocols
(if
available)
regarding
steps,
reaction
conditions,
scale.
review
is
structured
according
functional
group
formed
reaction.
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
51(2), P. 594 - 627
Published: Dec. 20, 2021
The
many
waves
of
biocatalysis
have
arisen
to
solve
long-standing
synthetic
challenges.
From
industrially
applied
hydrolases
enzymes
catalysing
selective
C–C-bond
formation,
enables
new
tools
access
a
plethora
compounds.
Chemical Society Reviews,
Journal Year:
2020,
Volume and Issue:
49(15), P. 5310 - 5358
Published: Jan. 1, 2020
This
review
highlights
the
developments
in
iron
and
cobalt
catalyzed
C(sp3)–H
bond
functionalization
reactions
with
emphasis
on
their
applications
organic
synthesis,
i.e.
natural
products
pharmaceuticals
synthesis
and/or
modification.
Natural Product Reports,
Journal Year:
2020,
Volume and Issue:
37(10), P. 1316 - 1333
Published: Jan. 1, 2020
Covering:
up
to
the
beginning
of
2020Enzymes
depending
on
cofactors
are
essential
in
many
biosynthetic
pathways
natural
products.
They
often
involved
key
steps:
catalytic
conversions
that
difficult
achieve
purely
with
synthetic
organic
chemistry.
Hence,
cofactor-dependent
enzymes
have
great
potential
for
biocatalysis,
condition
a
corresponding
cofactor
regeneration
system
is
available.
For
some
cofactors,
these
systems
require
multiple
steps;
such
complex
enzyme
cascades/multi-enzyme
(still)
challenging
vitro
biocatalysis.
Further,
artificial
analogues
been
synthesised
more
stable,
show
an
altered
reaction
range,
or
act
as
inhibitors.
The
development
bio-orthogonal
can
be
used
production
modified
products
vivo
ongoing
challenge.
In
light
recent
progress
this
field,
review
aims
provide
overview
general
strategies
involving
analogues,
and
systems;
highlighting
current
possibilities
application
using
most
common
cofactors.
Biotechnology Advances,
Journal Year:
2020,
Volume and Issue:
40, P. 107520 - 107520
Published: Jan. 23, 2020
Competitive
sustainable
production
in
industry
demands
new
and
better
biocatalysts,
optimized
bioprocesses
cost-effective
product
recovery.
Our
review
sheds
light
on
the
progress
made
for
individual
steps
towards
these
goals,
starting
with
discovery
of
enzymes
their
corresponding
genes.
The
are
subsequently
engineered
to
improve
performance,
combined
reaction
cascades
expand
scope
integrated
whole
cells
provide
an
optimal
environment
bioconversion.
Strain
engineering
using
synthetic
biology
methods
tunes
host
production,
design
optimizes
conditions
downstream
processing
ensures
efficient
recovery
commercially
viable
products.
Selected
examples
illustrate
how
modified
can
revolutionize
future-oriented
applications
ranging
from
bioproduction
bulk-,
specialty-
fine
chemicals,
active
pharmaceutical
ingredients
carbohydrates,
over
conversion
greenhouse-gas
CO2
into
valuable
products
biocontrol
agriculture,
recycling
polymers
precious
metals.
