toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques DOI

Chenjing Lu,

Yaying Huang,

Jian Cui

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 2295 - 2312

Published: July 13, 2024

Engineered Living Materials (ELMs) are materials composed of or incorporating living cells as essential functional units. These can be created using bottom-up approaches, where engineered spontaneously form well-defined aggregates. Alternatively, top-down methods employ advanced science techniques to integrate with various kinds materials, creating hybrids and intricately combined. ELMs blend synthetic biology science, allowing for dynamic responses environmental stimuli such stress, pH, humidity, temperature, light. exhibit unique "living" properties, including self-healing, self-replication, adaptability, making them highly suitable a wide range applications in medicine, conservation, manufacturing. Their inherent biocompatibility ability undergo genetic modifications allow customized functionalities prolonged sustainability. This review highlights the transformative impact over recent decades, particularly healthcare protection. We discuss current preparation methods, use endogenous exogenous scaffolds, assembly, 3D bioprinting, electrospinning. Emphasis is placed on ongoing research technological advancements necessary enhance safety, functionality, practical applicability real-world contexts.

Language: Английский

Engineered Living Materials For Sustainability DOI
Bolin An, Yan‐Yi Wang, Yuan‐Yuan Huang

et al.

Chemical Reviews, Journal Year: 2022, Volume and Issue: 123(5), P. 2349 - 2419

Published: Dec. 13, 2022

Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living (ELMs), which are composed matter or cell communities embedded self-regenerating matrices their own artificial scaffolds. Like natural such as bone, wood, skin, ELMs, possess the functional capabilities organisms, can grow, self-organize, self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, fabricating advanced smart materials. This review first introduces dynamic features systems potential developing novel We then summarize recent research progress on emerging design strategies from both perspectives. Finally, we discuss positive impacts promoting sustainability key future directions.

Language: Английский

Citations

122
Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability DOI Creative Commons
Charles Jo, Jing Zhang, Jenny M. Tam

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 19, P. 100560 - 100560

Published: Jan. 21, 2023

Filamentous fungi drive carbon and nutrient cycling across our global ecosystems, through its interactions with growing decaying flora their constituent microbiomes. The remarkable metabolic diversity, secretion ability, fiber-like mycelial structure that have evolved in filamentous been increasingly exploited commercial operations. industrial potential of fermentation ranges from the discovery bioproduction enzymes bioactive compounds, decarbonization food material production, to environmental remediation enhanced agricultural production. Despite fundamental impact ecology biotechnology, molds mushrooms not, to-date, significantly intersected synthetic biology ways comparable other cell factories (e.g. Escherichia coli,Saccharomyces cerevisiae, Komagataella phaffii). In this review, we summarize a suite computational tools for mining, engineering optimization as chassis. A combination methods genetic engineering, mutagenesis, experimental evolution, modeling can be used address strain development bottlenecks established emerging industries. These include slow mycelium growth rate, low production yields, non-optimal alternative feedstocks, difficulties downstream purification. scope biomanufacturing, then detail previous efforts improving key by targeting protein processing pathways, hyphae morphogenesis, transcriptional control. Bringing practices into hidden world will serve expand limited panel host organisms allow commercially-feasible environmentally-sustainable enzymes, chemicals, therapeutics, foods, materials future.

Language: Английский

Citations

46
Flexible Fungal Materials: Shaping the Future DOI
Antoni Gandía,

Jeroen G. van den Brandhof,

Freek V.W. Appels

et al.

Trends in biotechnology, Journal Year: 2021, Volume and Issue: 39(12), P. 1321 - 1331

Published: March 31, 2021

Language: Английский

Citations

102
Developments in bioprocess for bacterial cellulose production DOI
Reeta Rani Singhania, Anil Kumar Patel,

Yi-Sheng Tseng

et al.

Bioresource Technology, Journal Year: 2021, Volume and Issue: 344, P. 126343 - 126343

Published: Nov. 13, 2021

Language: Английский

Citations

98
Bacterial cellulose spheroids as building blocks for 3D and patterned living materials and for regeneration DOI Creative Commons
Joaquín Caro‐Astorga, Kenneth T. Walker,

Natalia Herrera

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Aug. 19, 2021

Engineered living materials (ELMs) based on bacterial cellulose (BC) offer a promising avenue for cheap-to-produce that can be programmed with genetically encoded functionalities. Here we explore how ELMs fabricated in modular fashion from millimetre-scale biofilm spheroids grown shaking cultures of Komagataeibacter rhaeticus. define reproducible protocol to produce BC the high yield producer K. rhaeticus and demonstrate first time their potential use as building blocks grow 3D shapes. Using engineered rhaeticus, functionalized these make patterned BC-based signal within material sense report chemical inputs. We also investigate method regenerate damaged way fuse together smaller sections synthetic into larger piece. This work improves our understanding spheroid formation showcases great fabricating, patterning repairing biomaterial cellulose.

Language: Английский

Citations

85
Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing DOI Creative Commons
Seokheun Choi

Small, Journal Year: 2022, Volume and Issue: 18(18)

Published: Feb. 4, 2022

Abstract Considerable research efforts into the promises of electrogenic bacteria and commercial opportunities they present are attempting to identify potential feasible applications. Metabolic electrons from enable electricity generation sufficient power portable or small‐scale applications, while quantifiable electric signal in a miniaturized device platform can be sensitive enough monitor respond changes environmental conditions. Nanomaterials produced by offer an innovative bottom‐up biosynthetic approach synergize bacterial electron transfer create effective coupling at cell–electrode interface. Furthermore, revolutionize field bioelectronics effectively interfacing electronics with microbes through extracellular transfer. Here, these new directions for their recent integration micro‐ nanosystems comprehensively discussed specific attention toward distinct applications powering, sensing, synthesizing. challenges individual strategies solutions provided valuable guidelines practical implementation. Finally, perspective view on how use hold immeasurable promise development future presented.

Language: Английский

Citations

54
Flexible bioelectronic device fabricated by conductive polymer–based living material DOI Creative Commons
Zenghao Wang, Haotian Bai, Wen Yu

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(25)

Published: June 22, 2022

Living materials are worked as an inside collaborative system that could naturally respond to changing environmental conditions. The regulation of bioelectronic processes in living be effective for collecting biological signals and detecting biomarkers. Here, we constructed a material with conjugated polymers poly[3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene chloride] (PMNT) Shewanella oneidensis MR-1 biofilm. In addition, the was integrated flexible device lactate detection physiological fluids (sweat, urine, plasma). Owing electroconductivity polymers, PMNT optimize process material. collected electrical signal wirelessly transferred portable smartphone reading analyzing. Because is also biomarker cancer treatment, further used detect count cells. proof using conductive polymer-based exhibits promising applications next-generation personal health monitoring systems.

Language: Английский

Citations

48
A de novo matrix for macroscopic living materials from bacteria DOI Creative Commons
Sara Molinari, Robert F. Tesoriero, Dong Li

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 21, 2022

Abstract Engineered living materials (ELMs) embed cells in a biopolymer matrix to create with tailored functions. While bottom-up assembly of macroscopic ELMs de novo would offer the greatest control over material properties, we lack ability genetically encode protein that leads collective self-organization. Here report growth from Caulobacter crescentus display and secrete self-interacting protein. This formed assembled into centimeter-scale ELMs. Discovery design principles allowed us tune composition, mechanical catalytic function these work provides genetic tools, rules, platform for growing both cellular structure function.

Language: Английский

Citations

46
Is net-zero feasible: Systematic review of cement and concrete decarbonization technologies DOI
Moncef L. Nehdi, Afshin Marani, Lei Zhang

et al.

Renewable and Sustainable Energy Reviews, Journal Year: 2023, Volume and Issue: 191, P. 114169 - 114169

Published: Dec. 12, 2023

Language: Английский

Citations

40
Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials DOI Creative Commons
Isabella M. Goodchild-Michelman, George M. Church, Max G. Schubert

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 19, P. 100583 - 100583

Published: Feb. 11, 2023

Cyanobacteria are ideal candidates to use in developing carbon neutral and negative technologies; they efficient photosynthesizers amenable genetic manipulation. Over the past two decades, researchers have demonstrated that cyanobacteria can make sustainable, useful biomaterials, many of which engineered living materials. However, we only beginning see such technologies applied at an industrial scale. In this review, explore ways synthetic biology tools enable development cyanobacteria-based biomaterials. First give overview ecological biogeochemical importance work has been done using create biomaterials so far. This is followed by a discussion commonly used strains exist engineer cyanobacteria. Then, three case studies-bioconcrete, biocomposites, biophotovoltaics-are explored as potential applications Finally, challenges future directions cyanobacterial discussed.

Language: Английский

Citations

26