Inhibition Control by Continuous Extractive Fermentation Enhances De Novo 2‐Phenylethanol Production by Yeast DOI Creative Commons

A N Brewster,

Arjan Oudshoorn,

Marion van Lotringen

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Current microbial cell factory methods for producing chemicals from renewable resources primarily rely on (fed-)batch production systems, leading to the accumulation of desired product. Industrially relevant like 2-phenylethanol (2PE), a flavor and fragrance compound, can exhibit toxicity at low concentrations, inhibit host activity, negatively impact titer, rate, yield. Batch liquid-liquid (L-L) In Situ Product Removal (ISPR) was employed mitigate inhibition effects, but not found sufficient industrial-scale application. Here, we demonstrated that continuous selective L-L ISPR provides solution maintaining productivity de novo produced 2PE an industrial pilot scale. A unique bioreactor concept called "Fermentation Accelerated by Separation Technology" (FAST) utilizes hydrostatic pressure differences separate aqueous- extractant streams within one unit operation, where both product extraction take place - allowing control concentration inhibiting compound. Controlled aqueous levels (0.43 ± 0.02 g kg

Language: Английский

Design principles for engineering bacteria to maximise chemical production from batch cultures DOI Creative Commons
Ahmad A. Mannan, Alexander P. S. Darlington, Reiko Tanaka

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Language: Английский

Citations

1
Technoeconomic analysis for near-term scale-up of bioprocesses DOI Creative Commons
Tuhin Poddar, Corinne D. Scown

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 92, P. 103258 - 103258

Published: Jan. 20, 2025

Language: Английский

Citations

0
Synthetic biology approaches to negative emissions technologies: a technological and ethical appraisal DOI Creative Commons

Dominic Y. Logel,

Josh Wodak

Frontiers in Climate, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 22, 2025

This article explores the impact that synthetic biology approaches may have on Negative Emissions Technologies (NETs). Synthetic has both altered and created biological pathways inspired by nature to develop new NETs sequester greenhouse gases into industrially useful chemicals, such as biomass calcium carbonate. However, continues encounter difficulties when implementing scaling up production due a combination of hard limits (within biology) ‘soft’ (of social economic costs). Additionally, NETs, along with Ecosystem in general, operate climate technofixes, wherein insufficient thought is given ethical quandaries arising from releasing designed organisms environment, even under controlled conditions. In this paper, we provide technological appraisal context change mitigation through Technology.

Language: Английский

Citations

0
Two-Stage Bioconversion of Cellulose to Single-Cell Protein and Oil via a Cellulolytic Consortium DOI Creative Commons
Eric Charles Peterson, Christian Hermansen,

Anna Marie Yong

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 72 - 72

Published: Feb. 2, 2025

A novel approach for converting non-edible plant biomass into single-cell protein and oil (SCPO) via consolidated bioprocessing has been established, leveraging aerotolerant thermophilic cellulolytic consortia consisting mainly of Thermoanaerobacterium thermosaccharolyticum, Sporolactobacillus spp. Clostridium sensu stricto to achieve the rapid complete conversion crystalline cellulose a consistent cocktail lactate, acetate ethanol. This is an excellent substrate cultivating organisms SCPO production food feed applications, including Cyberlindnera jadinii, Yarrowia lipolytica Corynebacterium glutamicum. Cultivation on this resulted in yields (YX/S) up 0.43 ± 0.012 g/g, indicating yield from (YX/Cellulose) 0.27 0.007 g/g (dwb). The resulting was rich (42.5% 57.9%), essential amino acids (27.8% 43.2%) lipids (7.9% 8.4%), with unsaturated fatty acid fractions 89%. Unlike fermentation feedstocks derived easily digested (i.e., waste), applied cellulosic biomass, mixed-culture bioconversion can be carried out without adding expensive enzymes. two-stage unlock as untapped feedstock production, potential strengthen resiliency circularity systems.

Language: Английский

Citations

0
Comprehensive evaluation of the capacities of microbial cell factories DOI Creative Commons
Gi Bae Kim,

Hyung Kyu Kim,

Sang Yup Lee

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 24, 2025

Systems metabolic engineering is facilitating the development of high-performing microbial cell factories for producing chemicals and materials. However, constructing an efficient factory still requires exploring selecting various host strains, as well identifying best-suited strategies, which demand significant time, effort, costs. Here, we comprehensively evaluate capacities propose strategies systems steps, including strain selection, pathway reconstruction, flux optimization. We analyze five representative industrial microorganisms production 235 different bio-based suggest most suitable corresponding chemical production. To improve innate capacity by more pathways, heterologous reactions, cofactor exchanges are systematically analyzed. Additionally, present include up- down-regulation target improved chemicals. Altogether, this study will serve a comprehensive resource in Constructing Here authors calculate maximum yields 5 microbes, evaluated reactions swaps, predicted providing engineering.

Language: Английский

Citations

0
Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation DOI Creative Commons
Maurice Hädrich,

Clarissa Scheuchenegger,

Sören-Tobias Vital

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 28, 2025

Abstract Background Pyruvate is a precursor for various compounds in the chemical, drug, and food industries therefore an attractive target molecule microbial production processes. The fast-growing bacterium Vibrio natriegens excels with its specific substrate uptake rate as unconventional chassis industrial biotechnology. Here, we aim to exploit traits of V. pyruvate fermentations low biomass concentrations. Results We inactivated dehydrogenase complex Δ vnp12 , which harbors deletions prophage regions . resulting strain aceE was unable grow minimal medium glucose unless supplemented acetate. In shaking flasks, showed growth 1.16 ± 0.03 h − 1 produced 4.0 0.3 g Pyr L within 5 h. optimized parameters aerobic fermentation process applied constant maintenance feed 0.24 Ac resulted maximal concentration only 6.6 0.4 CDW yielded highly active resting cells (q S ) 3.5 0.2 Glc −1 41.0 1.8 volumetric productivity 4.1 Carbon balancing disclosed gap 30%, identified partly parapyruvate. Deletion ligK encoding HMG/CHA aldolase did not impact formation but plasmid-based overexpression negatively affected led 3-fold higher parapyruvate culture broth. Notably, also supernatants pyruvate-producing Corynebacterium glutamicum strain. Cell-free bioreactor experiments mimicking biological formation, pointing chemical reaction contributing synthesis. Conclusions engineered metabolically producing high at concentration. However, found that accompanied by well C. Parapyruvate seems be result conversion might supported biochemically reaction.

Language: Английский

Citations

0
Environmental and Economic Benefits from Strain and Bioprocess Improvements for Sustainable Production of 3-Hydroxypropanoic Acid in a Sugarcane Biorefinery DOI Creative Commons
Manasseh K. Sikazwe, Sarel Johannes Marthinus Malherbe, Jeanne Louw

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
Semi-continuous biomanufacturing for maximizing the production of complex chemicals and fuels: a case study of amorpha-4,11-diene DOI Creative Commons
Carlos Rafael Castillo Saldarriaga,

Stephen Sarria,

Christine Nicole S. Santos

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

2
Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks DOI Creative Commons
Enrico Orsi,

Javier M Hernández-Sancho,

Maaike S Remeijer

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103195 - 103195

Published: Sept. 16, 2024

One-carbon (C1) feedstocks, such as carbon monoxide (CO), formate (HCO

Language: Английский

Citations

2
CIFR (Clone-Integrate-Flip-out-Repeat): a toolset for iterative genome and pathway engineering of Gram-negative bacteria DOI Creative Commons

Filippo Federici,

Francesco Luppino,

Clara Aguilar-Vilar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

ABSTRACT Advances in genome engineering enable precise and customizable modifications of bacterial species. Toolsets for metabolic that exhibit broad-host compatibility are particularly valued owing to their portability. Tn 5 transposon vectors have been widely used establish random integrations desired DNA sequences into genomes. However, the iteration procedure remains challenging because limited availability selection markers. Here, we present CIFR, a mini-Tn integration system iterative engineering. The pCIFR incorporate attP attB sites flanking an antibiotic resistance marker select insertion. Subsequent removal these determinants is facilitated by Bxb1 integrase, user-friendly counter-selection marker, both encoded auxiliary plasmids. Hence, CIFR delivers engineered strains harboring stable insertions free any cassette, allowing reusability tool. was validated Pseudomonas putida, Escherichia coli , Cupriavidus necator underscoring its portability across diverse industrially-relevant hosts. toolbox calibrated through combinatorial chromoprotein genes P. putida generating displaying color palette . Next, introduced carotenoid biosynthesis pathway two-step process, showcasing potential tool balancing. broad utility expands synthetic biology toolkit engineering, construction complex phenotypes, while opening new possibilities genetic manipulations.

Language: Английский

Citations

1