PLoS Pathogens,
Journal Year:
2016,
Volume and Issue:
12(12), P. e1006109 - e1006109
Published: Dec. 19, 2016
Type
IV
pilus
(T4P)
systems
are
complex
molecular
machines
that
polymerize
major
pilin
proteins
into
thin
filaments
displayed
on
bacterial
surfaces.
Pilus
functions
require
rapid
extension
and
depolymerization
of
the
pilus,
powered
by
assembly
retraction
ATPases,
respectively.
A
set
low
abundance
minor
pilins
influences
dynamics
unknown
mechanisms.
The
Vibrio
cholerae
toxin-coregulated
(TCP)
is
among
simplest
T4P
systems,
having
a
single
TcpB
lacking
ATPase.
Here
we
show
TcpB,
like
its
homolog
CofB,
initiates
assembly.
co-localizes
with
pili
but
at
extremely
levels,
equivalent
to
one
subunit
per
pilus.
We
used
micropillars
assay
demonstrate
TCP
retractile
despite
absence
ATPase,
relies
as
V.
tcpB
Glu5Val
mutant
fully
piliated
does
not
induce
movements.
This
impaired
in
TCP-mediated
autoagglutination
TcpF
secretion,
consistent
being
required
for
these
functions.
propose
incorporating
growing
Glu5-dependent
manner,
which
stalls
triggers
processive
disassembly.
These
results
provide
framework
understanding
filament
more
closely
related
II
secretion
system.
Proceedings of the National Academy of Sciences,
Journal Year:
2016,
Volume and Issue:
113(14)
Published: March 1, 2016
Significance
Bacterial
biofilms
are
ubiquitous
in
the
environment
and
serve
beneficial
roles
microbiota
communities
context
of
eukaryotic
hosts
industrial
applications,
yet
pathogenic
bacteria
can
also
cause
devastating
infections.
Biofilm
architectures
usually
studied
at
a
coarse
morphological
level,
consequently,
little
is
known
about
internal
biofilm
architecture
how
it
emerges.
Here,
we
use
an
optical
imaging
technique
to
visualize
every
cell
inside
thousands
Vibrio
cholerae
discover
architectural
transitions
major
phases
V.
growth.
Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(4), P. 696 - 696
Published: March 24, 2022
In
this
review,
the
general
background
is
provided
on
cyanobacteria,
including
morphology,
cell
membrane
structure,
and
their
photosynthesis
pathway.
The
presence
of
cyanobacteria
in
nature,
industrial
applications
are
discussed,
production
secondary
metabolites
explained.
Biofilm
formation,
as
a
common
feature
microorganisms,
detailed
role
diffusion
bacterial
colonization
described.
Then,
discussion
narrowed
down
to
cyanobacterium
Synechocystis,
lab
model
microorganism.
relation,
morphology
Synechocystis
discussed
its
different
elements
detailed.
Type
IV
pili,
complex
multi-protein
apparatus
for
motility
cell-cell
adhesion
described
underlying
function
phototaxis
behavior
cells,
response
homogenous
or
directional
illumination,
reported
relation
run
tumble
statistics
cells
emphasized.
suspensions,
there
may
exist
reciprocal
interaction
between
carrying
fluid.
effects
shear
flow
growth,
doubling
per
day,
biomass
production,
pigments,
lipid
reported.
Reciprocally,
rheological
properties
suspensions
addressed.
This
review
only
takes
up
grounds
does
not
get
into
biological
aspects
se.
Thus,
it
substantially
more
comprehensive
that
sense
than
other
reviews
have
been
published
last
two
decades.
It
also
written
researchers
field,
but
those
physics
engineering,
who
find
interesting,
useful,
related
own
research.
Nature Communications,
Journal Year:
2019,
Volume and Issue:
10(1)
Published: Sept. 13, 2019
Abstract
Candidate
phyla
radiation
(CPR)
bacteria
separate
phylogenetically
from
other
bacteria,
but
the
organismal
distribution
of
their
protein
families
remains
unclear.
Here,
we
leveraged
sequences
thousands
uncultivated
organisms
and
identified
that
co-occur
in
genomes,
thus
are
likely
foundational
for
lineage
capacities.
Protein
family
presence/absence
patterns
cluster
CPR
together,
away
all
archaea,
partly
due
to
proteins
without
recognizable
homology
bacteria.
Some
involved
cell-cell
interactions
potentially
important
episymbiotic
lifestyles.
The
diversity
combinations
may
exceed
Over
bacterial
tree,
broadly
recapitulate
phylogenetic
structure,
suggesting
persistence
core
sets
since
divergence.
could
have
arisen
an
episode
dramatic
heterogeneous
genome
reduction
or
a
protogenote
community
co-evolved
with
