The Vibrio cholerae Minor Pilin TcpB Initiates Assembly and Retraction of the Toxin-Coregulated Pilus DOI Creative Commons
Dixon Ng,

Tony Harn,

Tuba Altindal

et al.

PLoS Pathogens, Journal Year: 2016, Volume and Issue: 12(12), P. e1006109 - e1006109

Published: Dec. 19, 2016

Type IV pilus (T4P) systems are complex molecular machines that polymerize major pilin proteins into thin filaments displayed on bacterial surfaces. Pilus functions require rapid extension and depolymerization of the pilus, powered by assembly retraction ATPases, respectively. A set low abundance minor pilins influences dynamics unknown mechanisms. The Vibrio cholerae toxin-coregulated (TCP) is among simplest T4P systems, having a single TcpB lacking ATPase. Here we show TcpB, like its homolog CofB, initiates assembly. co-localizes with pili but at extremely levels, equivalent to one subunit per pilus. We used micropillars assay demonstrate TCP retractile despite absence ATPase, relies as V. tcpB Glu5Val mutant fully piliated does not induce movements. This impaired in TCP-mediated autoagglutination TcpF secretion, consistent being required for these functions. propose incorporating growing Glu5-dependent manner, which stalls triggers processive disassembly. These results provide framework understanding filament more closely related II secretion system.

Language: Английский

Cyclic diguanylate signaling in Gram-positive bacteria DOI Open Access
Erin B. Purcell, Rita Tamayo

FEMS Microbiology Reviews, Journal Year: 2016, Volume and Issue: 40(5), P. 753 - 773

Published: June 26, 2016

The nucleotide second messenger 3′-5′ cyclic diguanylate monophosphate (c-di-GMP) is a central regulator of the transition between motile and non-motile lifestyles in bacteria, favoring sessility. Most research investigating functions c-di-GMP has focused on Gram-negative species, especially pathogens. Recent work Gram-positive species revealed that plays similar roles Gram-positives, though precise targets mechanisms regulation may differ. majority bacterial life exists surface-associated state, with motility allowing bacteria to disseminate colonize new environments. signaling regulates flagellum biosynthesis production adherence factors appears be primary mechanism by which sense respond surfaces. Ultimately, influences ability bacterium alter its transcriptional program, physiology behavior upon surface contact. This review discusses how are able via flagella type IV pili, role regulating response surfaces, emphasis studies bacteria.

Language: Английский

Citations

72
Regulation and controlling the motility properties of Pseudomonas aeruginosa DOI Open Access
Fazlurrahman Khan, Dung Thuy Nguyen Pham, Sandra Oloketuyi

et al.

Applied Microbiology and Biotechnology, Journal Year: 2019, Volume and Issue: 104(1), P. 33 - 49

Published: Nov. 25, 2019

Language: Английский

Citations

69
DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction DOI
David W. Adams,

Sandrine Stutzmann,

Candice Stoudmann

et al.

Nature Microbiology, Journal Year: 2019, Volume and Issue: 4(9), P. 1545 - 1557

Published: June 10, 2019

Language: Английский

Citations

66
A Critical Evaluation of Bifidobacterial Adhesion to the Host Tissue DOI Creative Commons

Christina Westermann,

Marita Gleinser,

Sinéad C. Corr

et al.

Frontiers in Microbiology, Journal Year: 2016, Volume and Issue: 7

Published: Aug. 5, 2016

Bifidobacteria are common inhabitants of the human gastrointestinal tract that, despite a long history research, have not shown any pathogenic potential whatsoever. By contrast, some bifidobacteria associated with number health-related benefits for host. The reported beneficial effects include competitive exclusion pathogens, alleviation symptoms irritable bowel syndrome and inflammatory disease, modulation intestinal systemic immune responses. Based on these effects, widely used as probiotics by pharmaceutical dairy industries. In order to exert effect to, at least transiently, colonize host in sufficient population size. Besides other criteria such resistance manufacturing processes transit, probiotic bacteria tested adhesion structures including epithelial cells (IECs), mucus extracellular matrix (ECM) components. present review article, we summarize current knowledge bifidobacterial that mediate tissue compare similar bacteria. This reveals most adhesive mechanisms involved or even identical those employed pathogens cause disease. It is thus reasonable assume equally important commensal play role exerted bifidobacteria.

Language: Английский

Citations

65
The Vibrio cholerae Minor Pilin TcpB Initiates Assembly and Retraction of the Toxin-Coregulated Pilus DOI Creative Commons
Dixon Ng,

Tony Harn,

Tuba Altindal

et al.

PLoS Pathogens, Journal Year: 2016, Volume and Issue: 12(12), P. e1006109 - e1006109

Published: Dec. 19, 2016

Type IV pilus (T4P) systems are complex molecular machines that polymerize major pilin proteins into thin filaments displayed on bacterial surfaces. Pilus functions require rapid extension and depolymerization of the pilus, powered by assembly retraction ATPases, respectively. A set low abundance minor pilins influences dynamics unknown mechanisms. The Vibrio cholerae toxin-coregulated (TCP) is among simplest T4P systems, having a single TcpB lacking ATPase. Here we show TcpB, like its homolog CofB, initiates assembly. co-localizes with pili but at extremely levels, equivalent to one subunit per pilus. We used micropillars assay demonstrate TCP retractile despite absence ATPase, relies as V. tcpB Glu5Val mutant fully piliated does not induce movements. This impaired in TCP-mediated autoagglutination TcpF secretion, consistent being required for these functions. propose incorporating growing Glu5-dependent manner, which stalls triggers processive disassembly. These results provide framework understanding filament more closely related II secretion system.

Language: Английский

Citations

65