Regulatory Mechanisms and Promising Applications of Quorum Sensing-Inhibiting Agents in Control of Bacterial Biofilm Formation

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Oct. 15, 2020

A biofilm is an assemblage of microbial cells attached to a surface and encapsulated in extracellular polymeric substance (EPS) matrix. The formation one the important mechanisms bacterial resistance, which not only leads hard-to-control infections humans animals but also enables bacteria be major problem various fields, such as food processing, wastewater treatment metalworking. Quorum sensing (QS) cell-to-cell communication process that depends on population density mediated by small diffusible signaling molecules called autoinducers (AIs). Bacteria use QS regulate diverse arrays functions, including virulence formation. Therefore, interference with using inhibiting agents, inhibitors (QSIs) quorum quenching (QQ) enzymes, reduce or even completely repress pathogenic appears promising approach control infections. In this review, we summarize QS-regulating QS-inhibiting agents formation, strategies for discovery new current applications several fields provide insight into development effective drugs bacteria.

Language: Английский

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Exploring the antivirulence potential of phenolic compounds to inhibit quorum sensing in Pseudomonas aeruginosa

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 11, 2025

Language: Английский

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Roles of Two-Component Systems in Pseudomonas aeruginosa Virulence

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(22), P. 12152 - 12152

Published: Nov. 10, 2021

is an opportunistic pathogen that synthesizes and secretes a wide range of virulence factors.

Language: Английский

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Motility of Vibrio spp.: regulation and controlling strategies

Applied Microbiology and Biotechnology, Journal Year: 2020, Volume and Issue: 104(19), P. 8187 - 8208

Published: Aug. 20, 2020

Language: Английский

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Repurposing phytochemicals as anti‐virulent agents to attenuate quorum sensing‐regulated virulence factors and biofilm formation inPseudomonas aeruginosa

Microbial Biotechnology, Journal Year: 2021, Volume and Issue: 15(6), P. 1695 - 1718

Published: Nov. 29, 2021

Summary Unregulated consumption and overexploitation of antibiotics have paved the way for emergence antibiotic‐resistant strains ‘superbugs’. Pseudomonas aeruginosa is among opportunistic nosocomial pathogens causing devastating infections in clinical set‐ups globally. Its artillery equipped with diversified virulence elements, extensive antibiotic resistance biofilms has made it a ‘hard‐to‐treat’ pathogen. The pathogenicity P. modulated by an intricate cell density‐dependent mechanism called quorum sensing (QS). firmly controlled QS genes, their expression drives aggressiveness infection. Attempts to identify develop novel antimicrobials seen sharp rise past decade. Among different proposed mechanisms, anti‐virulence approach target pseudomonal virtue anti‐QS anti‐biofilm drugs appears occupy centre stage. In this respect, bioactive phytochemicals gained prominence scientific community owing significant quenching (QQ) properties. Recent studies shed light on QQ activities various other perturbing QS‐dependent . This review highlights recent evidences that reinforce application plant bioactives combating infections, advantages shortcomings therapy.

Language: Английский

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Friends or enemies? The complicated relationship between Pseudomonas aeruginosa and Staphylococcus aureus

Molecular Microbiology, Journal Year: 2021, Volume and Issue: 116(1), P. 1 - 15

Published: Feb. 12, 2021

Abstract Pseudomonas aeruginosa ( Pa ) and Staphylococcus aureus Sa are opportunistic pathogens that most commonly co‐isolated from chronic wounds the sputum of cystic fibrosis patients. Over last few years, there have been plenty contrasting results studies involving P. S. co‐cultures. The general concept outcompetes has challenged is more evidence now they can co‐exist. Nevertheless, it still remains difficult to mimic polymicrobial infections in vitro vivo. In this review, we discuss recent advances regard Pa‐Sa molecular interactions, their physical responses, vivo models. We believe important optimize growth conditions laboratory, determine appropriate bacterial starting ratios, consider environmental factors study co‐existence these two pathogens. Ideally, optimized media should reflect host‐mimicking with or without host cells allow both bacteria To further identify mechanisms could help treat complex infections, propose use relevant animal Ultimately, briefly how increase antibiotic tolerance.

Language: Английский

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Current concepts on Pseudomonas aeruginosa interaction with human airway epithelium

PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(3), P. e1011221 - e1011221

Published: March 30, 2023

Pseudomonas aeruginosa is a major, but opportunistic, respiratory pathogen, which rarely infects healthy individuals, mainly due to the barrier effect of human airway epithelium (HAE). This review explores interaction P. with HAE and progression infection. The basolateral part epithelium, includes membrane epithelial cells basement membrane, inaccessible in normal tight epithelia intact junctions. We highlight how exploits weaknesses gain access epithelium. crucial initiate infection observed injured repairing or chronically remodeled during extrusion senescent cell multiplication renewal. subsequent adhesion bacteria cytotoxic action virulence factors, including toxins delivered by type 3 secretion system (T3SS), lead retractions death. Eventually, progressively reaches propagates radially through basal disseminate using twitching flagellar motility.

Language: Английский

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Transcriptional Regulators Controlling Virulence in Pseudomonas aeruginosa

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 11895 - 11895

Published: July 25, 2023

Pseudomonas aeruginosa is a pathogen capable of colonizing virtually every human tissue. The host colonization competence and versatility this are powered by wide array virulence factors necessary in different steps the infection process. This includes involved bacterial motility attachment, biofilm formation, production secretion extracellular invasive enzymes exotoxins, toxic secondary metabolites, acquisition iron. Expression these during tightly regulated, which allows their only when they needed. process optimizes virulence. In work, we review intricate network transcriptional regulators that control expression P. aeruginosa, including one- two-component systems σ factors. Because inhibition holds promise as target for new antimicrobials, blocking trigger determinants promising strategy to fight clinically relevant pathogen.

Language: Английский

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In vitro investigation of relationship between quorum-sensing system genes, biofilm forming ability, and drug resistance in clinical isolates of Pseudomonas aeruginosa

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: March 25, 2024

Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen in the health-care systems and one of primary causative agents with high mortality hospitalized patients, particularly immunocompromised. The limitation effective antibiotic administration multidrug-resistant extensively drug-resistant P. isolates leads to development nosocomial infections health problems. Quorum sensing system contributes biofilm formation, expression bacterial virulence factors, drug resistance, causing prolonged patient infections. Therefore, due significance quorum increasing pathogenicity , objective our study was investigate frequency genes, as well formation resistance pattern among strains. Methods A total 120 were collected from different clinical specimens. disk diffusion method applied detect Also, microtiter plate carried out evaluate biofilm-forming ability isolates. Finally, rhlI rhlR lasI lasR genes examined by polymerase chain reaction method. Results In total, 88.3% found be multidrug-resistant, which 30.1% had pattern. highest lowest rates against ceftazidime (75.0%) ciprofloxacin (46.6%), respectively. 95.8% able produce biofilm, 42.5%, 33.3%, 20.0% strong, moderate, weak patterns, all strains follows: (81.6%), (90.8%), (89.1%), (78.3%). most common type related 94.3%. Furthermore, positive for However, gene both (83.0%) (90.6%) Moreover, (94.7%) (81.7%) prevalence isolates, Conclusion Our findings disclosed significantly a significant correlation indicating essential role this emergence caused .

Language: Английский

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Efflux pumps as potential targets for biofilm inhibition

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 23, 2024

Biofilms account for a great deal of infectious diseases and contribute significantly to antimicrobial resistance. Efflux pumps confer resistance microorganisms involve multiple processes biofilm formation. pump inhibitors (EPIs) are attracting considerable attention as inhibition strategy. The regulatory functions efflux in formation such mediating adherence, quorum sensing (QS) systems, the expression biofilm-associated genes have been increasingly identified. versatile properties both positive negative effects on Furthermore, function species-specific. Therefore, this review aims detail double-edged sword role provide potential targets give an overview EPIs

Language: Английский

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