Dual stressors of infection and warming can destabilize host microbiomes

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Climate change is causing extreme heating events and intensifying infectious disease outbreaks. Animals harbour microbial communities, which are vital for their survival fitness under stressful conditions. Understanding how microbiome structures in response to infection warming may be important forecasting host performance global change. Here, we evaluated alterations the microbiomes of several wild

Language: Английский

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Boron-dependent autoinducer-2-mediated quorum sensing stimulates the Cr(VI) reduction of Leucobacter chromiireducens CD49

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 375, P. 124290 - 124290

Published: Jan. 24, 2025

Language: Английский

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Warming During Different Life Stages has Distinct Impacts on Host Resistance Ecology and Evolution

Ecology Letters, Journal Year: 2025, Volume and Issue: 28(2)

Published: Feb. 1, 2025

ABSTRACT Climate change is increasing extreme heating events and the potential for disease outbreaks. Whether hosts can adapt to infection with rising temperatures important forecasting species persistence. We tested whether warming—at different host life stages—affects ecological evolutionary dynamics of resistance in Caenorhabditis elegans infected by a wild bacterial pathogen. competed resistant susceptible genotypes across 10 passages tracked spread population. Infection prolonged warming strongly selected genotype. Warming during development induced plastic defences against infection, reducing selective pressure costly genetic‐based resistance. Resistance was lost under ambient periodic warming. Selection likely weakened at dilution effect, whereby genotype reduced pathogen transmission. Evolutionary depend on balance among virulence, costs resistance, effect temperature stress.

Language: Английский

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Genome Sequencing of the Antibiotic-Resistant Leucobacter sp. HNU-1 and Its Developmental Toxicity in Caenorhabditis elegans

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3673 - 3673

Published: April 13, 2025

To date, Leucobacter species have been identified from diverse sources with various ecological and functional roles. However, the genomic features pathogenic potential of antibiotic-resistant strains remain understudied. Here, we isolated sp. HNU-1 tropical Hainan Province, China, found it can induce diapause in Caenorhabditis elegans following ingestion, while exhibiting no significant effects on nematode’s lifespan, survival rate, locomotion, intestinal epithelial cells. This bacterium demonstrates resistance to multiple antibiotics, including kanamycin, streptomycin, sulfonamides, vancomycin. On LB medium, forms yellow, opaque colonies a smooth, moist surface, regular edges, convex center, surrounding halo, diameters ranging 2 3 mm. Furthermore, performed whole-genome sequencing using third-generation high-throughput technology. De novo assembly revealed genome size 3,375,033 bp, GC content 70.37%. A total 3270 genes, accounting for 88.98% genome, were annotated, along six CRISPR sequences other genetic elements. Genomic bioinformatic analyses further antibiotics-related genes. research provides theoretical foundation investigating environmental bacteria environments offers new insights into therapeutic strategies microbial infections host–microbe interactions.

Language: Английский

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Biocontrol of Bacterial Wilt Disease Using Plant-Associated Bacterial Communities in Tomato

Molecular Plant-Microbe Interactions, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Host-protective or disease-suppressive microorganisms are emerging as sustainable solutions for controlling crop diseases, such bacterial wilt. However, the efficacy of biocontrol strategies is often constrained by limited resilience under varying environmental conditions and interactions with native microbial communities in field. One major challenge that introduced microbes face suppression indigenous due to competitive interactions. Synthetic (SynComs) offer a promising alternative strategy. conventional approaches assembling SynComs combining different isolates result antagonism competition among strains, leading ineffective inconsistent outcomes. In this study, we assembled wilt-suppressive SynCom tomato, composed derived from co-cultured complexes associated healthy plants. This demonstrates significant effects against Ralstonia pseudosolanacearum tomato seedlings both axenic soil conditions. Additionally, our findings suggest presence an optimal colonization level plants, which crucial effective disease suppression. The also exhibits direct antibiotic activity modulates plant-associated microbiome. Our results provide approach constructing consistent properties within community contexts. [Formula: see text] Copyright © 2025 Author(s). open access article distributed CC BY-NC-ND 4.0 International license .

Language: Английский

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Assessing multiple fecal sources to surf zone waters of two recreational beaches by bacterial community analysis

Water Research, Journal Year: 2022, Volume and Issue: 221, P. 118781 - 118781

Published: June 19, 2022

Fecal sources to recreational surf zone waters should be identified protect public health. While watershed origins of human and other fecal are often discoverable by quantitative polymerase chain reaction (qPCR) markers using spatially stratified samples, similarly assessing wastewater treatment plant (WWTP) outfall offshore contributions zones is challenged individual marker fate transport. Here, bacterial communities were assessed for relatedness between all hypothesized two urban California beaches, sequencing genes encoding 16S rRNA analyzing data SourceTracker FEAST. Ambient marine dominated the zone, while (human, dog, or gull) (sewage treated WWTP effluent) present at low proportions those from recycled water absent. Based on relative abundances genera specifically associated with feces, HF183 in community sequences, FEAST results when benchmarked HF183, major feces effluent. sequence (feces, sewage appeared uncorrelated previously obtained qPCR results, potential pathogen sequences elevated there more swimmers (i.e. during weekday afternoons, holidays busy weekends, race events), thus confirming previously-published qPCR-based conclusions that bather shedding contributed levels contamination. also showed evidence effluent an was entering thereby resolving a prior uncertainty. Thus, not only confirms HF183-based detections, but further allows which quantification can equivocal.

Language: Английский

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Source and variation of the amazing live Sea-Monkey microbiome

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(8), P. e0308763 - e0308763

Published: Aug. 12, 2024

An embryonic diapause in unfavourable conditions has allowed brine shrimp to thrive hypersaline environments and, unexpectedly, mail-order sachets and small, novelty tanks. Marketed as Sea-Monkeys®, each kit involves a 3-step process generate adult Artemia within matter of weeks. Whether these kits also allow for the maintenance host-associated microbiome is unclear. Therefore, comparing five replicate tanks under same culture conditions, we sequenced 16S ribosomal small subunit (SSU) gene analyse bacterial community compositions adults, their surrounding tank water, feed. Adult Sea-Monkeys® harboured that was clearly distinguishable from water food. Furthermore, individual had notable effect on fine-scale variation. Several Sea-Monkey variants appeared absent environmental samples included genera ( Leucobacter Microbacterium ) known confer desiccation resistance other hosts. Although taxonomy unclear, phylogenetic inference cytochrome c oxidase I (COXI) host animal suggests belong franciscana ‘superspecies’. Overall, appear be convenient scalable mesocosm study host-microbiome interactions could serve useful tool future invertebrate research, outreach, education.

Language: Английский

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Assessing the effectiveness of performic acid disinfection on effluents: focusing on bacterial abundance and diversity

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(48), P. 58252 - 58262

Published: Sept. 18, 2024

Poorly-treated wastewater harbors harmful microorganisms, posing risks to both the environment and public health. To mitigate this, it is essential implement robust disinfection techniques in treatment plants. The use of performic acid (PFA) oxidation has emerged as a promising alternative, due its powerful properties minimal environmental footprint. While PFA been used inactivate certain microbial indicators, potential tackle entire community effluents, particularly resistant bacterial strains, remains largely unexplored. present study evaluates efficacy on communities WWTP effluent, through resistance mechanisms their membrane structure. effluent microbiome was quantified identified. results showed that number damaged cells increases with CT, reaching maximum for CT = 240 mg/L•min plateauing around 60 mg/L•min, highlighting optimal conditions PFA-disinfection against viability. A low level 10-min contact time significantly affected composition. It worth noting sensitivity several genera such Flavobacterium, Pedobacter, Massilia, Exiguobacterium, Sphingorhabdus PFA, while others, Acinetobacter, Leucobacter, Thiothrix, Paracoccus, Cloacibacterium, resistance. detail groups correlated Gram-positive or Gram-negative These underline effectiveness reducing levels remodeling composition, even concentrations short times, demonstrating suitability widespread application WWTPs.

Language: Английский

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Warming during different life stages has distinct impacts on host resistance ecology and evolution

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 25, 2024

Global climate change is causing extreme heating events and intensifying infectious disease outbreaks. We tested whether warming (at various host life stages) could shape the ecological evolutionary trajectory of resistance, by competing nematode genotypes across 10 generations during infection a natural bacterial pathogen. found that persistent throughout development strongly favoured genetic-based resistance. Ambient temperatures or periodic within lifetime resulted in loss despite pathogen presence. Warming caused plastic temperature-mediated protection which weakened selection for more costly The findings an associated mechanistic model suggest dilution cells resistant hosts might help protect susceptible individuals when does not occur development. Host trajectories were likely driven combination fitness constraints on plasticity, condition-dependent virulence, effects.

Language: Английский

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Effects of the Microbial Action Network in the Humification of Industrial Composting of Chicken Manure

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Microbial activities is the key drive humification in aerobic composting. However, a comprehensive on how and functional taxa affect process efficiency still unclear. Here, we adopt network analysis, which can help to understand interactions between microorganisms mechanism underlying humification. According microbial it identified that Lactobacillus , Fastidiosipila other were belong central nodes. Firmicutes occupied most proportions symbiotic model, accounting for 59.48% of edges. Romboutsia taken into account as via analyzing relationships predicting functions within modules network. TOC, TN, C/N pH are primary environmental factors microorganisms. MO, VSS, TN TOC have positive effects all microorganisms, while has negative effect In particular, C / N an Bacteroides uniquely. By deeply exploring chicken manure industrial composting, this study paved way potential regulation composting by inoculating exogenous adjusting microenvironment factors. Furthermore, could provide implementation policy promoting reduction fertilizers, use organic fertilizer, reducing discharge pollutants fixed carbon.

Language: Английский

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