medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Sept. 19, 2023
The upper (URT) and lower (LRT) respiratory tract feature distinct environments responses affecting microbial colonization but investigating the relationship between them is technically challenging. We aimed to identify relationships taxa colonizing URT LRT explore their with development during childhood.
Language: Английский
Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)
Published: April 29, 2025
The mucosal system serves as the primary barrier against respiratory diseases and plays a crucial role in combating viral infections through immunity. resident microbial community constitutes main component of exerts significant inhibitory impact on invasion exogenous agents. However, precise relationship between microbiota, immunity, remains incomplete. This review aims to summarize regulatory interactions microbiota innate immune components such immunity trained By clarifying these complex relationships, this seeks identify potential targets for augmenting disease prevention strategies developing novel vaccine formulations. Furthermore, we propose possibility integrating fields microbiome-based therapeutics development create multifunctional formulations capable targeting induction. Such an approach holds great offering pathways treatment diseases.
Language: Английский
Citations
0
Cell Reports Medicine, Journal Year: 2023, Volume and Issue: 4(9), P. 101167 - 101167
Published: Aug. 25, 2023
Bacterial pneumonia is a considerable problem worldwide. Here, we follow the inter-kingdom respiratory tract microbiome (RTM) of unique cohort 38 hospitalized patients (n = 97 samples) with caused by Legionella pneumophila. The RTM composition characterized diversity drops early in hospitalization and ecological species replacement. RTMs highest bacterial fungal loads show low pathogen enrichment, suggesting high biomass as biomarker for secondary and/or co-infections. structure defined "commensal" cluster associated healthy "pathogen" enriched one, that equilibrium drives to recovery or dysbiosis. correlates disease severity co-morbidities, while clinical interventions influence dynamics. Fungi, archaea, protozoa seem contribute progress pneumonia. Thus, interplay equilibrium, load dynamics, play critical role patient recovery.
Language: Английский
Citations
8
iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110142 - 110142
Published: May 28, 2024
remains one of the deadliest infectious agents globally. Amidst efforts to control TB, long treatment duration, drug toxicity, and resistance underscore need for novel therapeutic strategies. Despite advances in understanding interplay between microbiome disease humans, specific role predicting susceptibility discriminating infection status tuberculosis still needs be fully investigated. We investigated impact
Language: Английский
Citations
2
Microbiological Research, Journal Year: 2024, Volume and Issue: 287, P. 127863 - 127863
Published: Aug. 3, 2024
High-throughput sequencing studies have shown that diet or antimicrobial treatments impact animal gut microbiota equilibrium. However, properties related to the microbial ecosystem stability, such as resilience, resistance, functional redundancy, must be better understood. To shed light on these ecological processes, we combined advanced statistical methods with 16 S rRNA gene sequencing, prediction, and fitness analyses in of cockroach Blattella germanica subject three periodic pulses antibiotic (AB) kanamycin (n=512). We first confirmed AB did not significantly affect cockroaches' biological fitness, changes were caused by insect physiology alterations. The sex variable was examined for time this species, no differences diversity composition found. comparison dynamics control treated populations revealed (1) treatment decreases completely disrupts co-occurrence networks between bacteria, altering community structure. (2) Although also affected genetic composition, redundancy would explain a smaller effect potential than taxonomic composition. (3) As predicted Taylor's law, generally most abundant taxa lesser extent less taxa. (4) Taxa follow different trends response ABs, highlighting "resistant taxa," which could critical restoration. (5) recovered faster after pulses, suggesting adapts repeated treatments.
Language: Английский
Citations
2
Veterinary Quarterly, Journal Year: 2024, Volume and Issue: 44(1), P. 1 - 18
Published: April 12, 2024
Complex respiratory diseases are a significant challenge for the livestock industry worldwide. These considerably impact animal health and welfare cause severe economic losses. One of first lines pathogen defense combines tract mucus, highly viscous material primarily composed mucins, thriving multi-kingdom microbial ecosystem. The microbiome-mucin interplay protects from unwanted substances organisms, but its dysfunction may enable pathogenic infections onset disease. Emerging evidence also shows that noncoding regulatory RNAs might modulate structure function relationship. This opinion paper unearths current understanding triangular relationship between microbiome, in context animals veterinary interest. There is need to look at these molecular underpinnings dictate distinct disease outcomes implement effective prevention, surveillance, timely intervention strategies tailored different epidemiological contexts.
Language: Английский
Citations
1
Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 121, P. 105606 - 105606
Published: May 19, 2024
We aimed to determine the importance of selection (mostly dependent on anthropogenic use antimicrobials) and transmission hygiene sanitation) as drivers spread antibiotic-resistant bacterial populations. The first obstacle estimating relative weight both independent variables is lack detailed quantitative data concerning number cells, potentially either pathogenic or harmless, species exposed antimicrobial action in microbiotas specific environments. second difficulty considering exerting their combined effects antibiotic resistance across eco-biological levels. As a consequence, advances are urgently required biology organismic resistance. absolute humans antibiotics commensal bacteria microbiomes should influence resistant "whole Earth" microbiome, with astonishingly high numbers cells species, which also antimicrobials various biogeographical spaces, shapes landscape. These spaces intensities bacteria. While waiting for more precise data, biostatistics analysis mathematical computational modeling can provide proxies compare In European countries lower sanitation levels, consumption plays major role increasing resistance; however, this not case Although linked, level varies according particular location. Therefore, interventions directed decrease be designed "a la carte" locations ecological conditions, including facilities.
Language: Английский
Citations
1
Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(6)
Published: June 1, 2024
Abstract Bdellovibrio bacteriovoru s HD100 is an obligate predatory bacterium that preys upon Gram‐negative bacteria. It has been proposed to be applied as a “living antibiotic” in several fields such agriculture or even medicine, since it able prey bacterial pathogens. Its interesting lifestyle makes this very attractive microbial chassis for co‐culture systems including two partners. A limitation goal the scarcity of suitable synthetic biology tools predator domestication. To fill gap, we have firstly adapted hierarchical assembly cloning technique Golden Standard (GS) make compatible with B. bacteriovorus HD100. The chromosomal integration Tn 7 transposon's mobile element, conjunction application GS technique, allowed systematic characterization repertoire constitutive and inducible promoters, facilitating control expression heterologous genes bacterium. PJ ExD /EliR proved exceptional promoter/regulator system when precise regulation essential, while promoter P BG37 showed high expression. These genetic represent step forward conversion into amenable strain biotechnology approaches.
Language: Английский
Citations
1
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: July 29, 2024
Over the past decade, advancements in high-throughput sequencing technologies have led to a qualitative leap our understanding of role microbiota human diseases, particularly oncology. Despite low biomass intratumoral microbiota, it remains crucial component tumor immune microenvironment, displaying significant heterogeneity across different tissues and individual patients. Although immunotherapy has emerged major strategy for treating tumors, patient responses these treatments vary widely. Increasing evidence suggests that interactions between system can modulate host responses, thereby influencing effectiveness immunotherapy. Therefore, is critical gain deep how shapes regulates microenvironment. Here, we summarize latest on cancer immunity, exploring potential mechanisms through which functions are influenced by within outside gut barrier. We also discuss impact response its clinical applications, highlighting future research directions challenges this field. anticipate valuable insights into immunity provided review will foster development microbiota-based therapies.
Language: Английский
Citations
1
Published: Feb. 1, 2024
We aimed to determine the importance of selection (mostly dependent on anthropogenic use antimicrobials) and transmission hygiene sanitation) as drivers spread antibiotic-resistant bacterial populations. The first obstacle estimating relative weight both independent variables is lack detailed quantitative data concerning number cells, potentially either pathogenic or harmless, species exposed antimicrobial action in microbiotas specific environments. second difficulty considering various parameters influencing antibiotic resistance across eco-biological levels that constitute an “organism-like” entity. As a consequence, advances are urgently required biology organismic resistance. absolute humans antibiotics commensal bacteria their microbiomes should influence resistant “whole Earth” microbiome, with astonishingly high numbers cells species, which also antimicrobials biogeographical spaces, shape landscape. These spaces intensities bacteria. While waiting for more precise data, biostatistics analysis mathematical computational modeling can provide proxies compare In European countries lower sanitation levels, consumption plays major role increasing resistance; however this not case levels. Although linked, level varies according particular location. Therefore, although interventions directed decrease be designed “à la carte,” global effects exposure local sites cannot underestimated.
Language: Английский
Citations
0
Frontiers in Pediatrics, Journal Year: 2024, Volume and Issue: 12
Published: June 7, 2024
In contrast to significant declines in deaths due lung cancer and cardiac disease Westernised countries, the mortality ‘chronic obstructive pulmonary disease’ (COPD) has minimally changed recent decades while ‘the incidence of bronchiectasis’ is on rise. The current focus producing guidelines for these two airway ‘diseases’ hindered progress both treatment prevention. elephant room that neither COPD nor bronchiectasis a but rather consequence progressive untreated inflammation. To make this case, it important review evolution our understanding how pathological appearance (bronchiectasis) an arbitrary physiological marker impaired airways came be labelled as ‘diseases’. Valuable insights into natural history can obtained from pre-antibiotic era. dramatic impacts antibiotics prevalence disease, especially childhood early adult life, have largely been forgotten will revisited misinterpretation trials undertaken those with chronic (bacterial) bronchitis. past decades, paediatricians observed increase what termed ‘persistent bacterial bronchitis’ (PBB). This condition shares all same characteristics bronchitis’, which prevalent young children during Additionally, radiological once again becoming more common and, recently, adults. Adult physicians remain sceptical about existence PBB; however, one study aimed at assessing efficacy adults persistent symptoms, researchers discovered majority patients exhibiting symptoms PBB were already long-term macrolides. there growing recognition importance respiratory microbiome ability bacteria persist potentially hostile environments through strategies such biofilms, intracellular communities, persister bacteria. challenging field likely require new approaches diagnosis treatment; needs embraced if real made.
Language: Английский
Citations
0