The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 DOI Creative Commons
Yue Wang, Xue Wang, Yuexia Wang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2022, Volume and Issue: 63(1), P. 37 - 37

Published: Jan. 27, 2022

Purpose: The oxygen-induced retinal neovascularization mouse model closely approximates pathological changes associated with human neovascularization-associated diseases, including retinopathies. We used this and endothelial cells (HRECs) under hypoxia to explore the relationship between taurine upregulated gene-1 (TUG1), vascular growth factor (VEGF), miR-299-3p on retinopathy of prematurity (ROP). Methods: An (OIR) was established; mice were divided into a normal control group, OIR TUG1 group (lentivirus control), TUG1-knockdown group. apoptosis evaluated using TUNEL assay. Angiogenic, apoptotic, inflammatory factors detected by Western blot, immunohistochemistry, immunofluorescence analyses. HRECs cultured assessed for VEGF expression, apoptosis, tubule formation, migration ability. TUG1, VEGF, via dual luciferase reporter gene Results: Intravitreal injection lentivirus reduced response in tissue markedly retina. Overexpression miR-299 rate, tube ability hypoxia-treated cells, thereby inhibiting formation new blood vessels. assay suggested that has binding sites VEGF. Conclusions: reduces expression VEGFA competitively adsorbing facilitates regulation neovascularization, suggesting it may serve as therapeutic target neovascular diseases.

Language: Английский

Precision and Advanced Nano-Phytopharmaceuticals for Therapeutic Applications DOI Creative Commons
Chooi Ling Lim,

Chandramathi Samudi Raju,

Tooba Mahboob

et al.

Nanomaterials, Journal Year: 2022, Volume and Issue: 12(2), P. 238 - 238

Published: Jan. 12, 2022

Phytopharmaceuticals have been widely used globally since ancient times and acknowledged by healthcare professionals patients for their superior therapeutic value fewer side-effects compared to modern medicines. However, phytopharmaceuticals need a scientific methodical approach deliver components thereby improve patient compliance treatment adherence. Dose reduction, improved bioavailability, receptor selective binding, targeted delivery of can be likely achieved molding them into specific nano-formulations. In recent decades, nanotechnology-based emerged as potential candidates the various communicable non-communicable diseases. Nanotechnology combined with broadens perspective overcomes problems associated plant medicine. The current review highlights application nano-phytopharmaceuticals in neurological, cardiovascular, pulmonary, gastro-intestinal disorders. We conclude that emerge promising therapeutics many pathological conditions good higher acceptance.

Language: Английский

Citations

26
Inhaled RNA drugs to treat lung diseases: Disease-related cells and nano–bio interactions DOI
Mengjun Zhang,

Haoyu Lu,

Liangkun Xie

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 203, P. 115144 - 115144

Published: Nov. 22, 2023

Language: Английский

Citations

14
CircRNA-associated ceRNA networks (circCeNETs) in chronic obstructive pulmonary disease (COPD) DOI
Zahra Firoozi, Abbas Shahi, Elham Mohammadisoleimani

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 349, P. 122715 - 122715

Published: May 11, 2024

Language: Английский

Citations

6
Bufei Yishen formula protects the airway epithelial barrier and ameliorates COPD by enhancing autophagy through the Sirt1/AMPK/Foxo3 signaling pathway DOI Creative Commons

Lidan Jia,

Xuefang Liu, Xinguang Liu

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Feb. 28, 2024

Abstract Object Bufei Yishen formula (BYF), a traditional Chinese medicine alleviates COPD symptoms and suppresses airway epithelial inflammation. In this study, we determined whether BYF protects the barrier from destruction in rats. Methods The protective effects of on were examined rat model. BEAS-2B cells exposed to cigarette smoke extract (CSE) determine effect function. Transcriptomic network analyses conducted identify mechanisms. Results Oral reduced severity rats by suppressing decline lung function, pathological changes, inflammation, protected function upregulating apical junction proteins, including occludin (OCLN), zonula occludens (ZO)-1, E-cadherin (E-cad). treatment permeability, increased TEER as well OCLN, ZO-1, E-cad CSE. Furthermore, 58 compounds identified used predict 421 potential targets. addition, expression 572 differentially expressed genes (DEGs) was CSE-exposed cells. A analysis targets DEGs revealed that regulates multiple pathways, which Sirt1, AMPK, Foxo3, autophagy pathways may be most important with respect Moreover, vitro experiments confirmed nobiletin, one active BYF, protein levels, E-cad. It also LC3B phosphorylated AMPK levels decreased phosphorylation FoxO3a. Conclusions enhancing through regulation SIRT1/AMPK/FOXO3 signaling pathway.

Language: Английский

Citations

5
“Nano-in-Micro” Structured Dry Powder Inhalers for pulmonary delivery: Advances and challenges DOI

Jinxue Cao,

Yuyi Xu,

Jian Zhang

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 96, P. 105648 - 105648

Published: April 6, 2024

Language: Английский

Citations

5
Maintenance of airway epithelial barrier integrity via the inhibition of AHR/EGFR activation ameliorates chronic obstructive pulmonary disease using effective-component combination DOI Creative Commons

Yanxin Wei,

Xuefang Liu, Yuhang Jiang

et al.

Phytomedicine, Journal Year: 2023, Volume and Issue: 118, P. 154980 - 154980

Published: July 17, 2023

Airway epithelial barrier dysfunction is highly related to the pathogenesis of chronic obstructive pulmonary disease (COPD). Effective-component combination (ECC) derived from Bufei Yishen formula (BYF) an effective treatment regimen for patients with COPD and has previously been found attenuate airway inflammation in rats.To determine mechanism underlying protective effects ECC-BYF against disruption COPD.The on were investigated a rat model. BEAS-2B cells stimulated cigarette smoke extract (CSE) direct function aryl hydrocarbon receptor (AHR)/ epidermal growth factor (EGFR) signaling.The results revealed that attenuated rats maintained by upregulating expression apical junction proteins, including occludin (OCC), zonula occludens (ZO)-1, E-cadherin (E-cad). In cells, decreased permeability, increased transepithelial electrical resistance, prevented decrease OCC, ZO-1, E-cad induced CSE exposure. addition, transcriptomics network analysis may be multiple signaling pathways, ErbB, AHR, PI3K-Akt-mTOR pathways. suppressed protein levels p-EGFR p-ERK1/2 mRNA CYP1A1 CSE-exposed as well p-EGFR, p-ERK1/2, lungs COPD. AHR agonist FICZ weakened effect suppressing increase ZO-1 OCC preventing inhibitory EGFR phosphorylation.This first study demonstrate function. The associated suppression AHR/EGFR pathway promote adhesion.

Language: Английский

Citations

11
Mechanisms of Bleomycin-induced Lung Fibrosis: A Review of Therapeutic Targets and Approaches DOI
Shaimaa M. Mohammed,

Haider Falih Shamikh Al-Saedi,

Amjed Qasim Mohammed

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(3), P. 1845 - 1870

Published: July 2, 2024

Language: Английский

Citations

4
Inhaled RNA Therapeutics for Obstructive Airway Diseases: Recent Advances and Future Prospects DOI Creative Commons
You Xu, Aneesh Thakur, Yibang Zhang

et al.

Pharmaceutics, Journal Year: 2021, Volume and Issue: 13(2), P. 177 - 177

Published: Jan. 28, 2021

Obstructive airway diseases, e.g., chronic obstructive pulmonary disease (COPD) and asthma, represent leading causes of morbidity mortality worldwide. However, the efficacy currently available inhaled therapeutics is not sufficient for arresting progression decreasing mortality, hence providing an urgent need development novel therapeutics. Local delivery to airways via inhalation promising drugs, because it allows directly target site action minimizes systemic drug exposure. In addition, modalities like RNA provide entirely new opportunities highly specific treatment diseases. Here, we review state art conventional drugs used COPD asthma with focus on quality attributes medicines, outline therapeutic potential safety drugs. Subsequently, present recent advances in manufacturing thermostable solid dosage forms administration, important inhalable dry powder formulations, obstacles translation clinic. Delivery challenges technologies overcome them are also discussed. Finally, future prospects RNA-based highlight major knowledge gaps, which require further investigation advance medicine towards bedside.

Language: Английский

Citations

26
Identification of Ferroptosis-Related Hub Genes and Their Association with Immune Infiltration in Chronic Obstructive Pulmonary Disease by Bioinformatics Analysis DOI Creative Commons
Yi-Can Yang, Mengyu Zhang, Jianyu Liu

et al.

International Journal of COPD, Journal Year: 2022, Volume and Issue: Volume 17, P. 1219 - 1236

Published: May 1, 2022

Ferroptosis and immune infiltration are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We aim to identify ferroptosis-related hub genes analyze their association with COPD through bioinformatics methods.The mRNA microarray data GSE38974 were downloaded from Gene Expression Omnibus obtain differentially expressed (DEGs). The DEGs intersected (FRGs) FerrDb FRGs. GO KEGG enrichment protein-protein interaction (PPI) analyses FRGs conducted R software STRING database. key module screened by Cytoscape software. MiRNAs, transcription factors signal molecules predicted miRNet NetworkAnalyst. correlation Comparative Toxicomics Database (CTD) receiver operating characteristic (ROC) curves analyzed. Immune was evaluated CIBERSORT algorithm. Spearman between infiltrated cells.Fifteen identified, which enriched some terms involving airway inflammatory responses structural remodeling. Five selected including HIF1A, IL6, PTGS2, CDKN1A ATM. Inference scores CTD indicated COPD. Two miRNAs, five one molecule predicted. combination could be a fine diagnostic indicator (AUC: 0.981, CI: 0.940-1.000). evaluation showed that monocytes M0 macrophages upregulated lung tissues, while CD8 T cells, activated NK M2 macrophages, resting dendritic cells mast downregulated. significantly associated cells.We identified (HIF1A, ATM) COPD, found they may influence regulating ferroptosis thus affecting infiltrating cells.

Language: Английский

Citations

19
Robust peptide/RNA complexes prepared with microfluidic mixing for pulmonary delivery by nebulisation DOI Creative Commons

Cheng Ma,

Michael Y.T. Chow,

Chengyang Zhang

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Small interfering RNA (siRNA) and messenger (mRNA) have drawn considerable attention in recent years due to their ability modulate the expression of specific disease-related proteins. However, it is difficult find safe, robust, effective delivery systems suitable for pulmonary treat lung diseases. In this study, two cationic peptides, namely LAH4-L1 PEG12KL4, were employed as non-viral vectors siRNA mRNA delivery. Four formulations (i.e. LAH4-L1/siRNA; PEG12KL4/siRNA; LAH4-L1/mRNA PEG12KL4/mRNA) investigated. Microfluidic mixing method was utilised fabricate complexes a controllable reproducible manner. Upon optimisation microfluidic protocol, vibrating mesh nebuliser aerosolise complexes, transfection efficiency evaluated on A549 BEAS-2B cells. Following nebulisation, inhalable mist generated all with mass median aerodynamic diameter below 5 μm. Although hydrodynamic particle sizes significantly reduced around 100 nm after nebulisation regardless original size prior binding vitro peptide successfully preserved no significant differences compared same system before nebulisation. The current result indicates that both PEG12KL4 hold potential future clinical application through

Language: Английский

Citations

0