Influence of macrophages and neutrophilic granulocyte-like cells on crystalline silica-induced toxicity in human lung epithelial cells DOI
Laurent Gaté,

Sylvie Sébillaud,

Mylène Lorcin

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 31, 2024

In many industrial activities, workers may be exposed by inhalation to particles that are aerosolized, To predict the human health hazard of these materials, we propose develop a co-culture model (macrophages, granulocytes, and alveolar epithelial cells) designed more representative inflammatory pulmonary response occurring in vivo. Phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 cells were used as macrophages, All-trans retinoic acid (ATRA)-differentiated HL60 granulocytes A549 type II cells. A crystalline silica sample DQ12 was prototypical particle for its capabilities induce DNA damage, response, oxidative stress cells; polyvinylpyridine-N-oxide (PVNO)-surface modified counterpart also negative particulate control. Cells mono-, bi- or tri-culture DQ12-PVNO 24 h. but not induced significant increase damage The presence differentiated reduced genotoxic effects this sample. exposure change interleukin-8 (IL-8) protein levels which exacerbated when THP-1, HL-60, added. addition, while no production TNFα detected monoculture, elevated cytokine observed systems. This work shows cell culture takes into consideration complexity might dependable study toxicological properties than "simple" monoculture models.

Language: Английский

Residential Proximity Land Use Characteristics and Exhaled Volatile Organic Compounds’ Impact on Pulmonary Function in Asthmatic Children DOI Creative Commons
Bo‐Yu Hsiao, Chun‐Sheng Huang, Chang‐Fu Wu

et al.

Journal of Xenobiotics, Journal Year: 2025, Volume and Issue: 15(1), P. 27 - 27

Published: Feb. 5, 2025

Background: Urban air pollution adversely affects children’s respiratory systems, but the impact of volatile organic compounds (VOCs) on pulmonary function remains unclear. This study aims to identify exhaled VOCs linked land use characteristics and reduced in asthmatic children, as well explore environmental thresholds influencing VOC exposure levels. Methods: We enrolled 97 aged 7 20, from Changhua County, Taiwan, collected personal residential data, samples, conducted tests. Land were derived addresses. used two models relationships between use, levels, function. Results: Our results show that m/p-xylene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene key contributors FEV1/FVC significantly predicted < 90% (AUC = 0.66; 95% CI: 0.53 0.79). These also major road areas within a 300 m buffer around homes. Conclusions: fills research gap low-level outdoor pediatric health, examining 1,2,4-trimethylbenzene, m/p-xylene potential biomarkers for impaired children.

Language: Английский

Citations

0
Toxicological Response of the BEAS‐2B Cell After Acute Exposure at the Air–Liquid Interface to Ethylbenzene and m‐Xylene Alone and in Binary Mixtures DOI Creative Commons

Nour Jaber,

Claude Emond, Fabrice Cazier

et al.

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

ABSTRACT Benzene, toluene, ethylbenzene, and xylenes (o‐, m ‐, p ‐xylenes) constitute a family, named BTEX, of volatile organic compounds (VOCs) known for its toxicity. This study aimed to the acute in vitro toxicity ethylbenzene ‐xylene on human bronchial epithelial cells exposed at air–liquid interface (ALI). The were VOCs alone mixture 1 h, followed by 5, 23, 47 h incubation. kinetics cell response was characterized, including cytotoxicity, xenobiotic biotransformation, antioxidant defense system, inflammatory response, apoptosis. gene expression results showed major differences between these two compounds, even though their chemical structure is very similar. Ethylbenzene did not appear be metabolized BEAS‐2B cells, as it inhibited metabolizing enzymes (XME) induce systems or However, slight observed after exposure. ‐Xylene inducing several XMEs upregulating involved well markers inflammation Co‐exposure binary resulted an inhibition phenomenon, resulting toxic action mechanisms studied. provide new information highlight importance conducting ALI exposures mixtures toxicants.

Language: Английский

Citations

0
Comparison of submerged and air liquid interface exposure: limitations and differences in the toxicological effects evaluated in bronchial epithelial cells DOI Creative Commons

Yamina Allouche,

Sara Marchetti, Rossella Bengalli

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: 279, P. 121856 - 121856

Published: May 14, 2025

Language: Английский

Citations

0
A Novel “Cells-on-Particles” Cytotoxicity Testing Platform In vitro: Design, Characterization, and Validation against Engineered Nanoparticle Aerosol DOI
Edvinas Krugly, Edvardas Bagdonas,

Jovilė Raudoniūtė

et al.

Toxicology, Journal Year: 2024, Volume and Issue: 508, P. 153936 - 153936

Published: Aug. 30, 2024

Language: Английский

Citations

2
The use of a complex tetra-culture alveolar model to study the biological effects induced by gold nanoparticles with different physicochemical properties DOI
Melissa Saibene, Tommaso Serchi, P Bonfanti

et al.

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 106, P. 104353 - 104353

Published: Dec. 30, 2023

Language: Английский

Citations

1
A mobile air-liquid interface exposure system optimized for outdoor toxicity testing of aerosols DOI Creative Commons
Ana Teresa Juárez-Facio, Micol Introna,

Sven Gustavsson

et al.

Aerosol Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Dec. 2, 2024

Particulate matter (PM) represents a growing concern in public health. Air Liquid Interface (ALI) exposure systems are used to assess the toxicity of airborne pollutants and their impact on human However, there have been only few studies using ALI outdoor environments. To address this need, we developed mobile system called ELectrostatic Air-Liquid Exposure (ELLIE) system. This is designed deposit particles, especially nanoparticles, its design has optimized enable field studies. Different particle size fractions can be targeted by different selectors. We several parameters, such as aerosol flow, temperature, humidity, incubation time, ensure best cell survival: while 100 mL/min doesn't viability no relative humidity (within tested range), even maintained at flows 214 when humidified least 85% humidity. The ELLIE controls temperature standard culture conditions (37 °C, 85–90% RH). Moreover, it enables an estimation deposited dose layer measuring distribution before after deposition layer. With method, copper nanoparticles was estimated 1.5 µg/cm2 study.

Language: Английский

Citations

0
Development of a dual PM2.5 sampling and direct exposure system incorporated an in vitro air–liquid interface culture method: Application to the northern Thailand haze season DOI

Pathinan Paengnakorn,

Nipapon Ngoyteja,

Molnapat Chuntama

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 125606 - 125606

Published: Dec. 1, 2024

Language: Английский

Citations

0
Influence of macrophages and neutrophilic granulocyte-like cells on crystalline silica-induced toxicity in human lung epithelial cells DOI
Laurent Gaté,

Sylvie Sébillaud,

Mylène Lorcin

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 31, 2024

In many industrial activities, workers may be exposed by inhalation to particles that are aerosolized, To predict the human health hazard of these materials, we propose develop a co-culture model (macrophages, granulocytes, and alveolar epithelial cells) designed more representative inflammatory pulmonary response occurring in vivo. Phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 cells were used as macrophages, All-trans retinoic acid (ATRA)-differentiated HL60 granulocytes A549 type II cells. A crystalline silica sample DQ12 was prototypical particle for its capabilities induce DNA damage, response, oxidative stress cells; polyvinylpyridine-N-oxide (PVNO)-surface modified counterpart also negative particulate control. Cells mono-, bi- or tri-culture DQ12-PVNO 24 h. but not induced significant increase damage The presence differentiated reduced genotoxic effects this sample. exposure change interleukin-8 (IL-8) protein levels which exacerbated when THP-1, HL-60, added. addition, while no production TNFα detected monoculture, elevated cytokine observed systems. This work shows cell culture takes into consideration complexity might dependable study toxicological properties than "simple" monoculture models.

Language: Английский

Citations

0