Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro DOI
Volodymyr Prokopіuk, Anatolii Onishchenko,

Yuliia Pazura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Abstract Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) Fura 2-AM staining reactive nitrogen species (RNS), detection caspases, lipid order-sensitive NR12S probe were employed evaluate viability, motility, death pathways, implication Ca2+, oxygen (ROS) RNS signaling, alterations membranes following exposure microflakes, respectively. To assess hemocompatibility Zn-containing osmotic fragility assay hemolysis performed, well multiple eryptosis parameters evaluated. Results. Our findings indicate dose-response ZnCH cells with no toxicity observed low concentrations (10 mg/L below). At high concentrations, promoted oxidative stress, Ca2+- caspase-dependent apoptosis altered membranes. Surprisingly, enucleated erythrocytes found lower compared cells. induced associated Ca2+ signaling recruitment caspases. Eryptosis assays more sensitive than evaluation hemolysis. Conclusions. show at indicating their potential

Language: Английский

Cell death signaling in human erythron: erythrocytes lose the complexity of cell death machinery upon maturation DOI Creative Commons
Anton Tkachenko, Ondřej Havránek

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Abstract Over the recent years, our understanding of cell death machinery mature erythrocytes has been greatly expanded. It resulted in discovery several regulated (RCD) pathways red blood cells. Apoptosis (eryptosis) and necroptosis share certain features with their counterparts nucleated cells, but they are also critically different particular details. In this review article, we summarize subroutines erythroid precursors (apoptosis, necroptosis, ferroptosis) comparison to (eryptosis erythronecroptosis) highlight consequences organelle clearance associated loss multiple components upon erythrocyte maturation. Recent advances role RCDs health disease have expanded potential clinical applications these lethal subroutines, emphasizing contribution development anemia, microthrombosis, endothelial dysfunction, as well diagnostic biomarkers markers storage-induced lesions. Fas signaling functional caspase-8/caspase-3 system not indispensable for eryptosis, might be retained mediate crosstalk between both erythrocyte-associated RCDs. The ability switch eryptosis suggests that is a simple unregulated mechanical disintegration, tightly controlled process. This allows investigation eventual pharmacological interventions aimed at individual erythrocytes.

Language: Английский

Citations

1
Recent Advances in the Evaluation of the Toxicological and Ecotoxicological Risks of Polymer, Zinc Oxide, Titanium Dioxide and Graphene Oxide Nanoparticles DOI Creative Commons
M. Pilar Vinardell

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(2), P. 97 - 97

Published: Jan. 9, 2025

Nanotechnology has substantial potential for advancements in the fields of biology and medicine [...]

Language: Английский

Citations

0
Utilizing Nanomaterials in Microfluidic Devices for Disease Detection and Treatment DOI Creative Commons
Zhenyu Tian, Yatian Fu, Zhaolong Dang

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(6), P. 434 - 434

Published: March 12, 2025

Microfluidic technology has gained widespread application in the field of biomedical research due to its exceptional sensitivity and high specificity. Particularly when combined with nanomaterials, synergy between two significantly advanced fields such as precision medicine, drug delivery, disease detection, treatment. This article aims provide an overview latest achievements microfluidic nanomaterials detection It delves into applications detecting blood parameters, cardiovascular markers, neurological tumor markers. Special emphasis is placed on their roles treatment, including models vessels, blood–brain barrier, lung chips, tumors. The development emerging medical technologies, particularly skin interactive devices imaging, also introduced. Additionally, challenges future prospects current clinical are discussed. In summary, play indispensable role With continuous advancement technology, will become even more profound extensive.

Language: Английский

Citations

0
Mn3O4 Nanocrystal-Induced Eryptosis Features Ca2+ Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes DOI Open Access

Yuriy Kot,

Volodymyr Prokopіuk, Vladimir Klochkov

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3284 - 3284

Published: April 1, 2025

Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed synthesized Mn3O4 NPs, identifying ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations up 20 mg/L were found be safe erythrocytes. Eryptosis assays shown more sensitive than fragility as markers Flow cytometry- confocal microscopy-based studies revealed induced by NPs was accompanied Ca2+ overload, altered homeostasis verified enhanced intracellular reactive oxygen species (ROS) nitrogen (RNS), a decrease lipid order cell membranes. Furthermore, NP-induced calpain- caspase-dependent.

Language: Английский

Citations

0
A Glutathione-Responsive L-Carnitine-Modified Camptothecin Oral Nano-Prodrug for Cancer Therapy DOI
Min Hong, Haobin Chen, Lei Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biocompatible PVA/gelatin and PVA/starch nanofibers incorporating sodium lactobionate obtained from enzymatic synthesis DOI
Aline Aniele Vencato, Naiara Jacinta Clerici, Marina Rigotti

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143112 - 143112

Published: April 1, 2025

Language: Английский

Citations

0
Exploring the effect of hydroxyapatite nanoparticle shape on red blood cells and blood coagulation DOI
Weitang Sun, Jiali Li,

Jingbin Zhong

et al.

Nanomedicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Sept. 23, 2024

In this study, we evaluated the effects of two types hydroxyapatite (HAP) nanoparticles, sharing same surface chemistry but differing in shape, on biological characteristics plasma, platelets and red blood cells.

Language: Английский

Citations

1
Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro DOI
Volodymyr Prokopіuk, Anatolii Onishchenko,

Yuliia Pazura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Abstract Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) Fura 2-AM staining reactive nitrogen species (RNS), detection caspases, lipid order-sensitive NR12S probe were employed evaluate viability, motility, death pathways, implication Ca2+, oxygen (ROS) RNS signaling, alterations membranes following exposure microflakes, respectively. To assess hemocompatibility Zn-containing osmotic fragility assay hemolysis performed, well multiple eryptosis parameters evaluated. Results. Our findings indicate dose-response ZnCH cells with no toxicity observed low concentrations (10 mg/L below). At high concentrations, promoted oxidative stress, Ca2+- caspase-dependent apoptosis altered membranes. Surprisingly, enucleated erythrocytes found lower compared cells. induced associated Ca2+ signaling recruitment caspases. Eryptosis assays more sensitive than evaluation hemolysis. Conclusions. show at indicating their potential

Language: Английский

Citations

0