Exploring the Role of Lower Genital Tract Microbiota and Cervical–Endometrial Immune Metabolome in Unknown Genesis of Recurrent Pregnancy Loss

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1326 - 1326

Published: Feb. 4, 2025

Recurrent pregnancy loss (RPL) of unknown genesis is a complex condition with multifactorial origins, including genetic, hormonal, and immunological factors. However, the specific mechanisms underlying endocervical cell proliferation disorders in women RPL remain inadequately understood, particularly concerning role microbiota viral infections. The aim this study was to investigate by examining microbiota, human papillomavirus (HPV) typing, expression levels key molecular biological markers, p16/Ki-67, BCL-2, miR-145, miR-34a. A prospective observational comparative executed on healthy pregnant controls full ethical approval. Samples were collected for HPV typing immunocytochemical analysis evaluate p16, Ki-67, anti-oncogenic microRNAs (miR-145 miR-34a). mRNA progesterone receptor (PGR-A) also assessed, alongside local immune status proinflammatory T-lymphocytes (Th17/Th1) regulatory CD4+ Tregs. Overexpression BCL-2 observed 52.5% who had an ASC-US/LSIL cytogram, average double p16/Ki-67 being three times higher than group. significant decrease PGR-A endocervix noted, accompanied dysregulated characterized increased prevalence Th17/Th1 cells reduction Additionally, miR-145 miR-34a endometrium significantly differed from physiological group, context high-risk infection. findings describe that are associated overexpression impaired regulation, altered microRNA profiles. These alterations may contribute pathophysiology RPL, highlighting need further research into targeted interventions could improve reproductive outcomes affected individuals.

Language: Английский

Assessment of sperm DNA integrity and ICSI outcome after sperm cryopreservation

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Journal Year: 2025, Volume and Issue: 77(3)

Published: Jan. 1, 2025

ABSTRACT The association between suboptimal sperm parameters and DNA damage in mature spermatozoa indicates that some individuals may have intrinsic issues with spermatogenesis. objective of this study is to evaluate the impact cryopreservation reactive oxygen species (ROS) on stability, as assessed by Sperm Chromatin Structure Assay (SCSA) motility viability, ICSI Result. Participants contacted Fertility Clinic provided written informed consent. All data were subsequently handled processed a confidential anonymous manner. There was source 300 participants (600 semen samples) study, divided into 3 groups. female partners underwent ICSI, (some oocytes injected fresh sperms others cryopreserved for same patient (liquid nitrogen at−196°C, accordance conventional protocols). Cryopreservation from fertile men does not seem adversely affect integrity either or prepared samples. However, morphology both samples significantly compromised process. No evidence cryopreserving healthy males would compromise genetic material laboratory-prepared

Language: Английский

Cytokine modulation and immunoregulation of uterine NK cells in pregnancy disorders

Cytokine & Growth Factor Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский