Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1320, P. 343004 - 343004
Published: July 28, 2024
Language: Английский
Chemical Science, Journal Year: 2023, Volume and Issue: 14(19), P. 4961 - 4978
Published: Jan. 1, 2023
A primer that covers the discovery and validation process for novel aptamers highlights recent applications of in biosensing cell separations.
Language: Английский
Citations
83
Talanta, Journal Year: 2023, Volume and Issue: 266, P. 124998 - 124998
Published: July 26, 2023
Language: Английский
Citations
83
Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1297, P. 342325 - 342325
Published: Feb. 2, 2024
Language: Английский
Citations
21
Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(50)
Published: Sept. 30, 2022
Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic easily modified with functional groups. These properties make acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. this Minireview, we will review historical evolution probes biosensors. We then specific examples nucleic-acid-based biosensors that have been evaluated clinical areas infectious disease, cancer, cardiovascular health.
Language: Английский
Citations
54
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 224, P. 115057 - 115057
Published: Jan. 2, 2023
Language: Английский
Citations
26
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(39), P. 14651 - 14658
Published: Sept. 18, 2023
Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the factors three library-immobilization SELEX (capture-SELEX, GO-SELEX, and gold-SELEX). We used spiked library that contained multiple DNA aptamers with different affinities adenosine. The separation efficiency was measured using qPCR, all showed very low release (<1%) in presence 100 μM Among these, barely any released from gold nanoparticles. Deep sequencing to compare aptamers: Ade1301, Ade1304, classical aptamer. Enrichment up 30 50-fold observed only capture-SELEX method, whereas other two below 1. By blocking primer-binding regions library, GO-SELEX reached 14% enrichment. Finally, based on nonspecific target-induced discussed, advantages were rationalized. Taken together, these results indicate much more efficient method enriching aptamers.
Language: Английский
Citations
24
Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: 42(19), P. 2391 - 2400
Published: May 28, 2024
Comprehensive Summary Interfacing DNA oligonucleotides and aptamers with gold nanoparticles has generated numerous functional hybrid materials for sensing, self‐assembly drug delivery applications. Our lab been working in this area 15 years. In article, the current understanding of adsorption to is summarized, related applications bioconjugation surface described. addition, problems using signaling aptamer binding are discussed. Finally, re‐selection previously reported targets library‐immobilization method reviewed. What most favorite original chemistry developed your research group? My work study biointerface between enabling rapid by lowering pH freezing. How do you get into specific field? Could please share some experiences our readers? PhD training was focused on catalytic detection metal ions, when I used signal reactions catalyzed DNA. When started my independent career University Waterloo 2009, realized that there were many fundamental issues regarding be studied. first teaching assignment a course named ‘Surfaces Interfaces’. By course, learned lot science concepts later research. That starting point me set up own program area. important personality scientific research? Curiosity, careful observation, critical thinking, keep trying. balance family? Research personal life don’t have always conflict. New ideas may spark am relaxed family environment. journals? Journal American Chemical Society , Angewandte Chemie International Edition Nucleic Acids Analytical Chemistry, Langmuir . hobbies? Running; traveling; watching movies.
Language: Английский
Citations
9
Advanced Composites and Hybrid Materials, Journal Year: 2025, Volume and Issue: 8(2)
Published: March 25, 2025
Language: Английский
Citations
1
ACS Chemical Biology, Journal Year: 2022, Volume and Issue: 17(8), P. 2121 - 2129
Published: Aug. 9, 2022
Since the report of RNA aptamer for theophylline, theophylline has become a key molecule in chemical biology designing switches and riboswitches. In addition, is an important drug treating airway diseases including asthma. The classic with excellent selectivity been used to design biosensors, although DNA aptamers are more desirable stability cost considerations. this work, we selected all top sequences shared same binding motifs. Binding was confirmed using isothermal titration calorimetry nuclease digestion assay, showing dissociation constant (Kd) around 0.5 μM theophylline. Theo2201 can be truncated down 23-mer while still Kd 9.8 μM. over caffeine 250,000-fold based on strand-displacement which than 20-fold higher compared aptamer. For other tested analogs, also showed better selectivity. Using structure-switching sensor method, detection limit 17 nM achieved selection buffer, 31 obtained 10% serum.
Language: Английский
Citations
38
Food Chemistry, Journal Year: 2022, Volume and Issue: 403, P. 134375 - 134375
Published: Sept. 21, 2022
Language: Английский
Citations
38