Catalysts,
Journal Year:
2022,
Volume and Issue:
12(12), P. 1659 - 1659
Published: Dec. 17, 2022
An
efficient
immobilization
of
Glucose
oxidase
(GOx)
on
an
appropriate
substrate
is
one
the
main
challenges
developing
fuel
cells
that
allow
energy
to
be
obtained
from
renewable
substrates
such
as
carbohydrates
in
physiological
environments.
The
research
importance
biofuel
relies
their
experimental
robustness
and
high
compatibility
with
biological
organisms
tissues
or
bloodstream
aim
obtaining
electrical
even
living
systems.
In
this
work,
we
report
use
5,10,15,20
tetrakis
(1-methyl-4-pyridinium)
porphyrin
glutathione
capped
CdTe
Quantum
dots
(GSH-CdTeQD)
a
support
matrix
for
GOx
carbon
surfaces.
Fluorescent
GSH-CdTeQD
particles
were
synthesized
characterization
by
UV-Vis
spectrophotometry
showed
particle
size
between
5–7
nm,
which
was
confirmed
DLS
TEM
measurements.
Graphite
Toray
paper
electrodes
modified
drop
coating
porphyrin,
GOx,
electrochemical
activity
toward
glucose
oxidation
evaluated
cyclic
voltammetry,
chronoamperometry
impedance
spectroscopy.
Additionally,
electrode
explored
scanning
microscopy,
finding
near
70%
surface
covered
active
enzyme.
sensitivity
0.58
±
0.01
μA/mM
apparent
Michaelis
constant
7.8
mM.
addition
BSA
blocking
protein
maintained
current
response
common
interferent
molecules
ascorbic
acid
(AA)
less
than
5%
interference
percentage.
Finally,
complex
employed
anodes
microfluidic
cell
(μBFC)
order
evaluate
performance
production.
enzymatic
used
μBFC
allowed
us
obtain
density
7.53
mAcm−2
at
maximum
power
2.30
mWcm−2;
open
circuit
potential
0.57
V
observed
cell.
results
suggest
immobilize
while
preserving
enzyme’s
catalytic
activity.
reported
arrangement
viable
option
bioenergy
production
and/or
quantification.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: April 4, 2023
Ovarian
cancer
stands
as
the
fifth
most
prevalent
among
women,
causing
more
mortalities
than
any
other
disease
of
female
reproductive
system.
There
are
numerous
histological
subtypes
ovarian
cancer,
each
which
has
distinct
clinical
characteristics,
risk
factors,
cell
origins,
molecular
compositions,
and
therapeutic
options.
Typically,
it
is
identified
at
a
late
stage,
there
no
efficient
screening
method.
Standard
therapies
for
newly
diagnosed
cytoreductive
surgery
platinum-based
chemotherapy.
The
difficulties
traditional
procedures
encourage
researchers
to
search
approaches,
such
nanotechnology.
Due
unique
characteristics
matter
nanoscale,
nanomedicine
emerged
potent
tool
creating
novel
drug
carriers
that
effective
have
fewer
adverse
effects
treatments.
Nanocarriers
including
liposomes,
dendrimers,
polymer
nanoparticles,
micelles
properties
in
surface
chemistry,
morphology,
mechanism
action
can
distinguish
between
malignant
normal
cells,
paving
way
targeted
delivery.
In
contrast
their
non-functionalized
counterparts,
development
functionalized
nano-formulations
with
specific
ligands
permits
selective
targeting
cancers
ultimately
increases
potential.
This
review
focuses
on
application
various
nanomaterials
treatment
diagnosis
advantages
over
conventional
methods,
role
controlled
delivery
systems
therapy
cancer.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(7), P. 336 - 336
Published: July 10, 2024
Cell
co-culture
technology
aims
to
study
the
communication
mechanism
between
cells
and
better
reveal
interactions
regulatory
mechanisms
involved
in
processes
such
as
cell
growth,
differentiation,
apoptosis,
other
cellular
activities.
This
is
achieved
by
simulating
complex
organismic
environment.
Such
studies
are
of
great
significance
for
understanding
physiological
pathological
multicellular
organisms.
As
an
emerging
cultivation
technology,
3D
based
on
microfluidic
chips,
can
efficiently,
rapidly,
accurately
achieve
co-culture.
accomplished
leveraging
unique
microchannel
structures
flow
characteristics
chips.
The
simulate
native
microenvironment
providing
a
new
technical
platform
studying
intercellular
communication.
It
has
been
widely
used
research
oncology,
immunology,
neuroscience,
fields.
In
this
review,
we
summarize
provide
insights
into
design
systems
detection
methods
employed
systems,
applications
these
models.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 23, 2024
Abstract
Biological
evaluation
of
biomedical
materials
faces
constraints
imposed
by
the
limitations
traditional
in
vitro
and
animal
experiments.
Currently,
miniaturized
biomimetic
microfluidic
technologies
organ‐on‐chip
systems
have
garnered
widespread
attention
field
drug
development.
However,
their
exploration
context
material
medical
device
development
remains
relatively
limited.
In
this
review,
a
summary
existing
biological
methods,
highlighting
respective
advantages
drawbacks
is
provided.
The
application
materials,
emphasizing
potential
as
highly
models
then
focused.
Finally,
challenges
opportunities
associated
with
utilizing
to
evaluate
are
discussed.
conclusion,
integration
advanced
presents
paradigm
shift
assessment
offering
prospective
more
accurate
predictive
devices.
SLAS TECHNOLOGY,
Journal Year:
2023,
Volume and Issue:
28(5), P. 302 - 323
Published: June 10, 2023
Efficient
sample
preparation
and
accurate
disease
diagnosis
under
field
conditions
are
of
great
importance
for
the
early
intervention
diseases
in
humans,
animals,
plants.
However,
in-field
high-quality
nucleic
acids
from
various
specimens
downstream
analyses,
such
as
amplification
sequencing,
is
challenging.
Thus,
developing
adapting
lysis
acid
extraction
protocols
suitable
portable
formats
have
drawn
significant
attention.
Similarly,
techniques
detection
methods
also
been
explored.
Combining
these
functions
an
integrated
platform
has
resulted
emergent
sample-to-answer
sensing
systems
that
allow
effective
analyses
outside
a
laboratory.
Such
devices
vast
potential
to
improve
healthcare
resource-limited
settings,
low-cost
distributed
surveillance
food
agriculture
industries,
environmental
monitoring,
defense
against
biological
warfare
terrorism.
This
paper
reviews
recent
advances
technologies
facile
/
or
could
be
adopted
into
novel
devices.
In
addition,
developments
challenges
commercial
kits
targeting
on-site
plant
discussed.
Applied and Environmental Microbiology,
Journal Year:
2025,
Volume and Issue:
91(1)
Published: Jan. 14, 2025
Electroactive
organisms
contribute
to
metal
cycling,
pollutant
removal,
and
other
redox-driven
environmental
processes
via
extracellular
electron
transfer
(EET).
Unfortunately,
developing
genotype-phenotype
relationships
for
electroactive
is
challenging
because
EET
necessarily
removed
from
the
cell
of
origin.
Microdroplet
emulsions,
which
encapsulate
individual
cells
in
aqueous
droplets,
have
been
used
study
a
variety
phenotypes
but
not
applied
investigate
EET.
Here,
we
describe
development
microdroplet
emulsion
system
sort
enrich
EET-capable
complex
populations.
We
validated
our
using
model
electrogen
Shewanella
oneidensis
described
tooling
benchtop
microfluidic
oxygen-limited
conditions.
demonstrated
enrichment
strains
exhibiting
mixed
wild-type
EET-deficient
As
proof-of-concept
application,
collected
samples
iron
sedimentation
Town
Lake
(Austin,
TX)
subjected
them
enrichment.
measured
an
increase
sorted
population
that
was
distinct
compared
growing
bulk
culture
with
Fe(III)
as
sole
acceptor.
Finally,
two
bacterial
species
previously
shown
be
EET-capable,
Cronobacter
sakazakii
Vagococcus
fessus,
were
further
cultured
characterized
electroactivity.
Our
results
demonstrate
utility
emulsions
isolating
identifying
bacteria.IMPORTANCEThis
work
outlines
new
high-throughput
method
bacteria
play
key
roles
trafficking,
soil
remediation,
degradation.
Many
existing
methods
are
coupled
microbial
growth
fitness-as
result,
contributions
weak
or
poor-growing
electrogens
often
muted.
However,
(EET)
has
historically
difficult
since
reduction
trace
back
parent
there
no
suitable
fluorescent
readouts
circumvents
these
challenges
by
utilizing
wherein
single
statistically
isolated
pico-
nano-liter-sized
droplet.
Then,
fluorescence
obtained
copper
reduction,
can
fluorescently
gated
performance.
Utilizing
technique,
characterize
unrecognized
fessus
sakazakii.
Trends in Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 1, 2025
The
biofilm
mode
of
growth
and
drug
efflux
are
both
important
factors
that
impede
the
treatment
bacterial
infections
with
antimicrobials.
Decades
work
have
uncovered
mechanisms
involved
in
biofilm-mediated
antimicrobial
tolerance,
but
links
between
these
phenomena
only
recently
been
discovered.
Novel
findings
show
how
impacts
global
cellular
physiology
antibiotic
underpinned
by
phenotypic
heterogeneity.
In
addition
can
mediate
cell-to-cell
interactions,
relevant
biofilms,
via
including
signaling
molecules
metabolites,
using
pump
components
establishment
local
gradients
pumping.
These
recent
suggest
tolerance
closely
coupled,
synergistic
effects
leading
to
evolution
resistance
environment.
