Realizing the multifunctional microfluidic flow manipulation based on hydrodynamic metamaterials

Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(2)

Published: Feb. 1, 2024

From their initial application to the fields of chemistry and biology, microfluidic chips used as micro total analysis systems have developed into new technologies satisfy requirements various societal industries. Microchannels are essential components chips; they play a vital role in connecting inlet outlet well determining flow distribution reagent mixing. Microfluidic research has always been devoted minimizing energy dissipation, fluid resistance, pressure drop realize energy-efficient chips. This study proposes theory for manipulating microchannels based on hydrodynamic metamaterials according spatial transformation theory. In particular, specifically designed construct shifters, splitters, combiners, theoretical numerical simulations performed assess performance. The systematic design metamaterial devices proposed this work establishes framework achieve steady state without inducing unstable disturbances complex-shape microchannels.

Language: Английский

---

A SAW‐Based Programmable Controlled RNA Detecting Device: Rapid In Situ Cytolysis‐RNA Capture‐RNA Release‐PCR in One Mini Chamber

Advanced Science, Journal Year: 2024, Volume and Issue: 11(29)

Published: May 21, 2024

Abstract Viral RNA detection is crucial in preventing and treating early infectious diseases. Traditional methods of require a large amount equipment technical personnel. In this study, proposed programmable controlled surface acoustic wave (SAW)‐based detecting device has been proposed. The can perform the entire viral process, including cell lysis by cell‐microparticle collision through SAW‐induced liquid whirling, capture SAW‐suspended magnetic beads, elution high streaming force, PCR thermal cycling SAW‐generated heat. completed all steps one mini chamber, requiring only 489 µl reagents for extraction, much smaller than used manual extraction (2065 µl). experimental results have shown that from are comparable to those achieved via commercial qPCR instrumental detection. This work demonstrated potential SAW‐based lab‐on‐a‐chip devices point‐of‐care testing provided novel approach rapidly

Language: Английский

---

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Published: April 3, 2024

Achieving effective control over microbial contamination necessitates the precise and concurrent identification of numerous pathogens. In this research, we have devised a remarkably sensitive duplex droplet digital PCR (dddPCR) reaction system to simultaneously detect Pseudomonas aeruginosa (P. aeruginosa) fragi fragi). Employing comparative genomics, identified four genes P. fragi. By specific analysis, RS22680 gene was selected as detection target LasR chosed aeruginosa, which were applied construct dddPCR reaction. terms specificity, sensitivity anti-interference ability, constructed verified analyzed. The assay showed excellent applicability, evidenced by limit 100 CFU/mL. When concentration natural background bacteria in milk or fresh meat times that bacteria, method still capable completing absolute quantification. simulation actual sample contamination, could be detected after 3 h enrichment culture, 6 h. established ddPCR exhibits exceptional performance, serving foundation for simultaneous various pathogenic food products.

Language: Английский

---

Smartphone-based plant-wearable microfluidic sensor with self driven electrolyte for in-situ detection of methyl parathion

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 418, P. 136254 - 136254

Published: July 4, 2024

Language: Английский

---

Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing

Advanced Science, Journal Year: 2024, Volume and Issue: 11(39)

Published: July 23, 2024

Graphene field-effect transistors (GFETs) are widely used in biosensing due to their excellent properties biomolecular signal amplification, exhibiting great potential for high-sensitivity and point-of-care testing clinical diagnosis. However, difficulties complicated fabrication steps the main limitations further studies applications of GFETs. In this study, a modular technique is introduced construct microfluidic GFET biosensors within 3 independent steps. The low-melting metal electrodes intricate flow channels incorporated maintain structural integrity graphene facilitate subsequent sensing operations. as-fabricated biosensor demonstrates long-term stability, performs effectively various ion environments. It also exhibits high sensitivity selectivity detecting single-stranded nucleic acids at 10 fm concentration. Furthermore, when combined with CRISPR/Cas12a system, it facilitates amplification-free rapid detection concentration 1 fm. Thus, believed that modular-fabricated may shed light on development FET-based applications.

Language: Английский

---

Multifunctional and structural magnetic nanobranches for high-efficiency biological detection

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163297 - 163297

Published: May 1, 2025

Language: Английский

---

The development of droplet-based microfluidic virus detection technology for human infectious diseases

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(7), P. 971 - 978

Published: Jan. 1, 2024

Virus-based human infectious diseases have a significant negative impact on people's health and social development. The need for quick, accurate, early viral infection detection in preventive medicine is expanding. A microfluidic control particularly suitable point-of-care-testing virus diagnosis due to its advantages of low sample consumption, quick speed, simple operation, multi-functional integration, small size, easy portability. It also thought development potential wide range application prospects the research technology. In an effort aid researchers creating novel tools detection, this review highlights recent developments droplet-based microfluidics discusses challenges opportunities rapid detection.

Language: Английский

---

Rapid DNA extraction and microfluidic LAMP system in portable equipment for GM crops detection

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 411, P. 135716 - 135716

Published: March 29, 2024

Language: Английский

---

Research progress and application exploration of typical microreactor technologies for health monitoring and disease Diagnosis/Treatment

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 155938 - 155938

Published: Sept. 1, 2024

Language: Английский

---

Rapid Amplification and Detection of Single‐Stranded Nucleic Acids for Point‐of‐Care Diagnosis

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract Nucleic acid detection plays a crucial role in various applications, including disease diagnostics, research development, food safety, and environmental health monitoring. A rapid, point‐of‐care (POC) nucleic test can greatly benefit healthcare system by providing timely diagnosis for effective treatment patient management, as well supporting diseases surveillance emerging pandemic diseases. Recent advancements acids technology have led to rapid assays single‐stranded that be integrated into simple miniaturized platforms ease of use. In this review, the study focuses on developments isothermal amplification, hybridization circuits, enzyme‐based signal reporting mechanisms, show promise POC testing. The also evaluates critical technical breakthroughs identify advantages disadvantages these methods applications.

Language: Английский

---