An amplification-free digital droplet assay for influenza A viral RNA based on CRISPR/Cas13a

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Scheme of the amplification-free digital droplet assay. Two innovations: (1) polystyrene capture beads to concentrate target RNA from a dilute sample and (2) “confinement” effect amplifies concentration molecule.

Language: Английский

Advances of 3D Cell Co-Culture Technology Based on Microfluidic Chips

Biosensors, Journal Year: 2024, Volume and Issue: 14(7), P. 336 - 336

Published: July 10, 2024

Cell co-culture technology aims to study the communication mechanism between cells and better reveal interactions regulatory mechanisms involved in processes such as cell growth, differentiation, apoptosis, other cellular activities. This is achieved by simulating complex organismic environment. Such studies are of great significance for understanding physiological pathological multicellular organisms. As an emerging cultivation technology, 3D based on microfluidic chips, can efficiently, rapidly, accurately achieve co-culture. accomplished leveraging unique microchannel structures flow characteristics chips. The simulate native microenvironment providing a new technical platform studying intercellular communication. It has been widely used research oncology, immunology, neuroscience, fields. In this review, we summarize provide insights into design systems detection methods employed systems, applications these models.

Language: Английский

Immuno-PCR: A high-sensitivity approach for biomarker analysis

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120289 - 120289

Published: April 1, 2025

Language: Английский