Imaged capillary isoelectric focusing and online mass spectrometry for milk whey protein characterization in dairy products

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: 699, P. 115765 - 115765

Published: Jan. 6, 2025

Language: Английский

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An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: high-efficient fractionation and online mass spectrometry detection

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100204 - 100204

Published: Jan. 1, 2025

Language: Английский

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Enabling icIEF Peak Identification of AAV Capsid Proteins by Fractionation on MauriceFlex and Subsequent Analysis by LC–MS

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

ABSTRACT A significant limitation of imaged capillary electric focusing (icIEF) is the inability to identify and characterize specific species in electropherogram. This has led development complementary ion‐exchange chromatography (IEX)‐based methods that are amenable either fraction collection subsequent characterization or online IEX coupled mass spectrometry. To overcome this while maintaining use icIEF, novel approaches, including an icIEF separation fractionation technology (MauriceFlex, ProteinSimple), have been developed. approach enables various peaks, which can then be characterized by spectrometry confirm identity separated charged species. Herein, MauriceFlex was applied adeno‐associated viral (AAV) gene therapy products, contain a DNA transgene packaged into protein capsid shown tremendous therapeutic potential recent years. Utilizing system, we developed for from AAV proteins (VP) liquid (LC–MS). When applying same sample preparation, charge profiles on instrument were demonstrated consistent with those original Maurice platform, industrial gold standard. Optimization VP method required low concentration samples, optimization mobilization conditions, enhancement recovery, maintenance stability post fractionation. able successfully collect charge‐separated samples subsequently analyze them MS analysis. In addition, workflow based downstream LC–MS established confirmation additional heterogeneity.

Language: Английский

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Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Electrophoresis, Journal Year: 2023, Volume and Issue: 44(17-18), P. 1279 - 1341

Published: Aug. 3, 2023

Abstract This review is in support of the development selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for electrophoresis: method validation pharmaceutical biological applications,” pointing out which fundamentals are still valid at same time showing enormous achievements last 25 years. The structures both reviews widely similar, order to facilitate their simultaneous use. Focusing on applications, successful use CE now demonstrated by more than 600 carefully selected references. Many those recent reviews; therefore, significant overview about field provided. There extra sections sample pretreatment related microchip CE, completely revised section protein analytes biomolecules general. general strategies summed with regard selectivity, efficiency, precision, analysis time, limit detection, requirements, validation.

Language: Английский

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A comprehensive review of the electrochemical advanced oxidation processes: Detection of free radical, Electrode materials and Application

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 113778 - 113778

Published: Aug. 6, 2024

Language: Английский

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Mass spectrometry study on SARS-CoV-2 recombinant vaccine with comprehensive separation techniques to characterize complex heterogeneity

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1297, P. 342349 - 342349

Published: Feb. 12, 2024

Language: Английский

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Pesticide residues in animal-derived food: Current state and perspectives

Food Chemistry, Journal Year: 2023, Volume and Issue: 438, P. 137974 - 137974

Published: Nov. 11, 2023

Language: Английский

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 165 - 198

Published: Sept. 5, 2023

Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.

Language: Английский

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Liquid phase detection in the miniature scale. Microfluidic and capillary scale measurement and separation systems. A tutorial review

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1305, P. 342507 - 342507

Published: March 23, 2024

Microfluidic and capillary devices are increasingly being used in analytical applications while their overall size keeps decreasing. Detection sensitivity for these microdevices gains more importance as device sizes consequently, sample volumes, decrease. This paper reviews optical, electrochemical, electrical, mass spectrometric detection methods that applicable to scale microfluidic devices, with brief introduction the principles each case. Much of this is considered context separations. We do consider theoretical aspects separations by open tubular liquid chromatography, arguably most potentially fertile area has been left fallow largely because lack scale-appropriate methods. also examine basis zone electrophoretic Optical discussed include UV/Vis absorbance, fluorescence, chemiluminescence refractometry. Amperometry essentially only electrochemical method microsystems. Suppressed conductance especially contactless conductivity (admittance) wide use ionic analytes. integrated various spectrometers, including ESI-MS, APCI-MS, MALDI-MS discussed. advantages disadvantages compare best reported limits uniform a format available information allows. While review pays attention recent developments, our primary focus on novelty ingenuity approach, regardless when it was first proposed, long can be relevant miniature platforms.

Language: Английский

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Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization (ESI) for protein heterogeneity characterization

Analytical Biochemistry, Journal Year: 2023, Volume and Issue: 680, P. 115312 - 115312

Published: Sept. 6, 2023

Language: Английский

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