Greening Separation and Purification of Proteins and Peptides

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(19)

Published: Oct. 1, 2024

The increasing awareness of environmental issues and the transition to green analytical chemistry (GAC) have gained popularity among academia industry in recent years. One principles GAC is reduction replacement toxic solvents with more sustainable environmentally friendly ones. This review gives an overview advances applying as alternative traditional organic for peptide protein purification analysis by liquid chromatography (LC) capillary electrophoresis (CE) methods. feasibility using greener LC CE methods demonstrated through several application examples; however, there still plenty room new developments fully realize their potential address existing challenges. Thanks tunable properties designer solvents, such ionic liquids deep eutectic almost infinite possible mixtures components production, it that some could potentially surpass harmful future. Therefore, future research should focus mainly on developing solvent combinations enhancing instruments be able effectively work solvents.

Language: Английский

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Antimicrobial Peptides From Different Sources: Isolation, Purification, and Characterization to Potential Applications

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(23)

Published: Dec. 1, 2024

ABSTRACT Antimicrobial peptides (AMPs) are excellent promising candidates for biomedical applications owing to their structural properties, high biocompatibility, good biodegradability, and functional diversity. Unlike conventional antibiotics, AMPs have been shown broad‐spectrum antimicrobial activity toward Gram‐positive/negative bacteria, as well antifungal antiviral activity. These also found be cytotoxic sperm cancer cells. A range of has isolated from various organisms, such fungi, plants, animals. This review summarizes the latest studies on AMPs, covering isolation, purification, characterization potential beyond.

Language: Английский

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Suberin as a green surfactant additive for peptide analysis using capillary electrophoresis

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1745, P. 465774 - 465774

Published: Feb. 13, 2025

Language: Английский

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Amino-β-cyclodextrin derivatives with different chain lengths as chiral selectors for separation of dansyl amino acid enantiomers by capillary electrophoresis

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1746, P. 465781 - 465781

Published: Feb. 14, 2025

Language: Английский

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Surfactant-based coatings for protein separation by capillary electrophoresis - A review

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343945 - 343945

Published: March 1, 2025

Language: Английский

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Advances in capillary electrophoresis for plant analysis

Talanta, Journal Year: 2025, Volume and Issue: 293, P. 128171 - 128171

Published: April 17, 2025

Language: Английский

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Microchip Electrophoretic Analyses of Cationic Analytes Using Large-Volume Dual Preconcentration by Isotachophoresis and Stacking (LDIS) – Field-Amplified Sample Injection (FASI) in Y-Form Channels

Chromatography, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

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Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Published: April 29, 2024

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage formation, these maladies effectively treated, leading to successful outcomes; curative therapies halt illnesses from advancing, thus improving quality life, and long-term survival patient. Still cutting-edge upgrades precision technologies necessary for early, reliable, affordable, rapid disease detection, but also vital well-being people future global public health. The emerging role utility non-invasive repeatable diagnostic test approaches detection health conditions been liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator signature (e.g., proteins), provide valuable information individual’s wellness and/or Among bodily secretions used diagnostics is sputum, complex viscous gel-like biopolymeric network, gained growing recognition as rich source biomarkers airway infections, pulmonary diseases, serves determinant reveal other illnesses. per World Health Organization, burden respiratory exacerbated by factors, ranging considerable subjection air pollution occupational contaminants, tobacco smoking second-hand smoke, addition poor socio-economic status. Due likely increase determinants, tract rise, affecting many individuals, putting stress healthcare facilities services worldwide. We therefore highlight need use expectorated induced sputum specimens routine sample testing protein diagnose chronic maladies, predict inflammation progression, well monitor effectiveness treatments. Further, we discuss urgency fast reliable point-of-care methods detect quantify crucial specimens, limitations faced when dealing with matrices.

Language: Английский

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Application of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) on straight-channel microchips

Analytical Sciences, Journal Year: 2024, Volume and Issue: 40(9), P. 1611 - 1617

Published: May 16, 2024

In this study, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) which is an on-line sample technique coupling with electroosmotic flow pump (LVSEP) transient (tITP) was applied to microchip electrophoresis (MCE) for improving both detection sensitivities peak shapes. To realize LDIS in MCE, we investigated experimental procedures injecting a short plug of leading electrolyte (LE) solution into straight microchannel without any sophisticated injector apparatus. We found that LE could be injected sample-filled straight-channel only making the liquid level outlet reservoir higher than inlet one. By applying reversed-polarity voltage microchip, anionic analytes throughout were first enriched LVSEP, followed tITP. Through second effect tITP LDIS, sensitivity enhancement factor (SEF) asymmetry standard dye improved from 878 0.62 1330 1.14, respectively, relative those conventional LVSEP. It should noted more viscous running buffer containing sieving polymers employed analysis, effective SEF separation efficiencies, especially bio-polymeric compounds. Finally, oligosaccharide protein analyses resulting SEFs 1410 ca. 50 maltotriose bovine milk casein, respectively.

Language: Английский

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Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

International Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 4(2), P. 309 - 333

Published: May 31, 2024

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage formation, these disorders effectively treated, leading to successful outcomes; curative therapies halt illnesses from advancing, thus improving quality life and long-term survival patient. Still, cutting-edge upgrades precision technologies necessary for early, reliable, affordable, rapid disease detection, but also vital well-being people future global public health. The emerging role utility non-invasive repeatable diagnostic test approaches detection health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator signature (e.g., proteins) provide valuable information an individual’s wellness and/or Among bodily secretions used diagnostics is sputum, complex viscous gel-like biopolymeric network gained growing recognition as rich source biomarkers airway infections pulmonary diseases, serves determinant reveal other illnesses. per World Health Organization, burden respiratory exacerbated factors ranging considerable subjection air pollution occupational contaminants tobacco smoking second-hand smoke, addition poor socio-economic status. Due likely increase determinants, tract rise, affecting many individuals, putting stress healthcare facilities services worldwide. aim this study was perform narrative review sputum constituents with emphasis proteins glycoproteins assessed possible lung organ diseases. A search conducted using mucus, proteomics, biomarkers, point-of-care testing keywords employing Google, PubMed (MEDLINE), Web Science, selecting most referenced related papers last decade. We, therefore, highlight need use expectorated induced specimens routine sample protein diagnose chronic disorders, predict inflammation progression, well monitor effectiveness treatments. Further, we discuss urgent fast reliable methods detect quantify crucial specimens, limitations faced when dealing matrices.

Language: Английский

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