ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(46), P. 64297 - 64306
Published: Nov. 8, 2024
Alzheimer's
disease
(AD)
is
a
widely
prevalent
neurodegenerative
condition
globally,
arousing
significant
interest
in
the
noninvasive
early
detection
of
disease.
The
concentration
amyloid
β
(Aβ)
biomarkers
blood
closely
linked
to
progression
AD,
emphasizing
importance
developing
precise
method
for
detecting
these
samples
diagnosis.
In
this
study,
we
developed
ratiometric
electrochemical
aptamer-based
(EAB)
biosensor
accurate
Aβ42
and
Aβ40.
utilizes
gold
nanoparticle
(AuNPs)
modified
electrode
through
an
deposition
assay
self-assembled
poly-A
based
U-shaped
structure
achieve
signal
changes
competitive
binding
target
molecules.
Importantly,
using
calibration
clipping
reference
probe,
proposed
displayed
ability
Aβ40,
with
limits
59
21
pM,
respectively.
It
also
has
great
stability
anti-interference
complex
biological
samples.
Furthermore,
it
demonstrated
satisfactory
performance
Aβ
peptides
20%
FBS
sample,
recoveries
ranging
from
96.7%
102.2%
serum
samples,
relative
standard
deviations
(RSD)
3.3%
8.9%.
Moreover,
expected
directly
quantify
Aβ42/Aβ40
ratio
by
introducing
suitable
providing
potential
effective
tool
diagnosis
AD.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(11), P. 987 - 987
Published: Nov. 17, 2023
Alzheimer’s
disease
(AD)
is
closely
related
to
neurodegeneration,
leading
dementia
and
cognitive
impairment,
especially
in
people
aged
>
65
years
old.
The
detection
of
biomarkers
plays
a
pivotal
role
the
diagnosis
treatment
AD,
particularly
at
onset
stage.
Field-effect
transistor
(FET)-based
sensors
are
emerging
devices
that
have
drawn
considerable
attention
due
their
crucial
ability
recognize
various
ultra-low
concentrations.
Thus,
FET
broadly
manipulated
for
AD
biomarker
detection.
In
this
review,
an
overview
typical
features
operational
mechanisms
described
detail.
addition,
summary
applicability
biosensors
research
field
outlined
discussed.
Furthermore,
trends
future
prospects
diagnostic
applications
also
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(6), P. 279 - 279
Published: May 29, 2024
As
a
typical
biomarker
of
Alzheimer's
disease,
rapid
and
specific
detection
tau
protein
can
help
improve
the
early
diagnosis
prognosis
disease.
In
this
study,
simple
sandwich
electrochemical
immunosensor
was
developed
for
protein.
Primary
monoclonal
antibodies
(mAb1)
against
middle
domain
(amino
acids
189-195)
were
immobilized
on
gold
electrode
surface
through
self-assembled
monolayer
(SAM)
3,3'-dithiobis
(sulfosuccinimidyl
propionate)
(DTSSP).
Then
captured
adsorption
between
antigen
antibody,
resulting
in
change
impedance.
Secondary
(mAb2)
N-terminal
region
used
further
amplification
binding
reaction
mAb1
A
linear
correlation
total
impedance
logarithm
concentration
found
from
2
×
10
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(42)
Published: Sept. 19, 2024
Abstract
The
colloidal
gold
nanoparticle
(AuNP)‐based
colorimetric
lateral
flow
assay
(LFA)
is
one
of
the
most
promising
analytical
tools
for
point‐of‐care
disease
diagnosis.
However,
low
sensitivity
and
insufficient
accuracy
still
limit
its
clinical
application.
In
this
work,
a
machine
learning
(ML)‐optimized
LFA
with
ultrasound
enrichment
developed
to
achieve
sensitive
accurate
detection
tau
proteins
early
screening
Alzheimer's
(AD).
device
integrated
portable
ultrasonic
actuator
rapidly
enrich
microparticles
using
ultrasound,
which
essential
sample
pre‐enrichment
improve
sensitivity,
followed
by
ML
algorithms
classify
predict
enhanced
signals.
results
undiluted
serum
testing
show
that
protocol
enables
efficient
classification
quantification
AD
biomarker
protein
concentration
an
average
98.11%
prediction
99.99%,
achieving
(LOD)
as
10.30
pg
mL
−1
.
Further
(POCT)
human
plasma
samples
demonstrates
potential
use
in
trials.
Such
reliable
immunosensor
high
precision
superb
sensing
performance
expected
put
perspective
diagnostic
platform.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Sept. 27, 2023
Abstract
Alzheimer’s
disease
(AD)
is
a
progressive
brain
disorder
characterized
by
the
ongoing
decline
of
functions.
Studies
have
revealed
detrimental
effects
hyperphosphorylated
tau
(p-tau)
protein
fibrils
in
AD
pathogenesis,
highlighting
importance
this
factor
early-stage
detection
conditions.
We
designed
an
electrochemical
immunosensor
for
quantitative
cis
conformation
p-tau
(
-p-tau)
employing
platinum
nanoparticles
(Pt
NPs)
supported
on
zeolitic
imidazolate
frameworks
(ZIF)
modifying
glassy
carbon
electrode
(GCE)
surface.
Under
optimum
conditions,
selectively
and
sensitively
detected
cis-p-tau
within
broad
linear
range
1
fg
mL
−1
to
10
ng
low
limit
(LOD)
with
desired
reproducibility
stability.
Furthermore,
fabricated
immunosensor's
performance
was
examined
analysis
serum
patients,
indicating
its
accuracy
feasibility
real-sample
analysis.
Notably,
first
application
Pt@ZIF-8
nanocomposite
fabricating
valid
selective
detection,
even
presence
trans-p-tau.
It
worth
mentioning
that
enzyme-linked
immunosorbent
assay
(ELISA)
reference
technique
not
able
evaluate
pico-
or
femtomolar
concentrations
cis-p-tau,
making
superior
measurement
screening
AD.
