Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-Like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes DOI Creative Commons
Ting Sun, Xinyao Yi, Lin Liu

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(13), P. 3000 - 3000

Published: June 24, 2024

The sensitivity of immunoassays is generally limited by the low signal reporter/recognition element ratio. Nanomaterials serving as carriers can enhance loading number reporters, thus improving detection sensitivity. However, general immobilization strategies, including direct physical adsorption and covalent coupling, may cause random orientation conformational change in proteins, partially or completely suppressing enzymatic activity molecular recognition ability. In this work, we proposed a strategy to load elements antibodies enzyme labels using boronic acid-modified metal-organic frameworks (MOFs) nanocarriers for amplification. conjugation was based on boronate ester interactions between carbohydrate moieties enzymes acid MOFs. Both MOFs could catalyze oxidation 3,3′,5,5′-tetramethylbenzidine (TMB) H2O2, therefore achieving dual To indicate feasibility strategy, colorimetric prostate specific antigen (PSA) were performed with Cu-MOFs peroxidase mimics TMB antibody (horseradish peroxidase, HRP). According absorbance intensity oxidized (oxTMB), PSA at concentration range 1~250 pg/mL be readily determined. addition, work presented site-specific oriented modification nanolabels which should valuable design novel biosensors high selectivity.

Language: Английский

Efficient Separation-Free and Wash-Free Immunoassay for Sensitive Detection of Biomarkers Based on the Distance-Driven Chemiluminescent Technique DOI
Xu Teng,

Lingyan Gui,

Weiyuan Liang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

The wash-free method represents a promising strategy for enhancing the detection efficiency of automated chemiluminescent (CL) immunoassay analyzers. Herein, novel separation-free and was developed first time based on distance-driven CL technique. In immunoassay, well-designed Au–Co metal nanoclusters (NCs) exhibited excellent peroxidase-like activity good stability, allowing efficient catalysis luminol or its analogue (ABEI)-H2O2 system even at low concentrations. Furthermore, large specific surface area NCs facilitated accommodation greater number antibodies, thereby capture antigens achieving dual amplification signal. technique relied formation sandwich-type immunocomplexes. Upon generation hydroxyl radicals superoxide anion through catalytic decomposition H2O2 by NCs, ABEI within immunocomplexes could efficiently react with these radicals, leading to significant enhancement in signals. C-reactive protein (CRP) chosen as model analyte evaluate practicability proposed immunoassay. Notably, presented high sensitivity, selectivity, reproducibility, successfully determining CRP serum samples recoveries 96.55–106.29%. Accordingly, advantage separation-free, wash-free, reliable characteristics drastically simplify operation steps enhance efficiency, which would make advances revolution traditional

Language: Английский

Citations

1

Gold Nanoparticles-Based Colorimetric Immunoassay of Carcinoembryonic Antigen with Metal–Organic Framework to Load Quinones for Catalytic Oxidation of Cysteine DOI Creative Commons

Zhao-Jiang Yu,

Tingting Yang, Gang Liu

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(20), P. 6701 - 6701

Published: Oct. 18, 2024

This work reported gold nanoparticles (AuNPs)-based colorimetric immunoassay with the Cu-based metal–organic framework (MOF) to load pyrroloquinoline quinone (PQQ) for catalytic oxidation of cysteine. In this method, both Cu2+ and PQQ in MOF could promote inducer cysteine by redox cycling, thus limiting cysteine-induced aggregation AuNPs achieving dual signal amplification. Specifically, recombinant carcinoembryonic antigen (CEA) targets were anchored on through metal coordination interactions between hexahistidine (His6) tag CEA unsaturated sites MOF. The CEA/PQQ-loaded be captured antibody-coated ELISA plate catalyze However, once target samples bound antibody immobilized surface, attachment would limited. Cysteine remaining solution trigger cause a color change from red blue. concentration was positively related AuNPs. signal-on competitive plasmonic exhibited low detection limit linear range 0.01–1 ng/mL. Note that most proteins commercial kits are His6 N- or C-terminal, so provide sensitive platform biomarkers.

Language: Английский

Citations

5

A Time-Resolved Fluorescent Microsphere Immunochromatographic Assay for Determination of Vitamin B12 in Infant Formula Milk Powder DOI Creative Commons
Qianqian Lü,

Yongwei Feng,

Qi Zhou

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 65 - 65

Published: Jan. 21, 2025

Vitamin B12 (VB12) is an important nutrient, and its quality control in food crucial. In this study, based on the principle of specific recognition target analyte by monoclonal antibodies (mAbs), a time-resolved fluorescent microsphere immunochromatographic assay (TRFM-ICA) was developed to detect content VB12 infant formula milk powder. First, performance anti-VB12 mAb evaluated, revealing half-maximal inhibitory concentration 0.370 ng/mL, affinity constant 2.604 × 109 L/mol no cross-reactivity with other vitamins. Then, highly sensitive TRFM-ICA developed, visual limit detection 10 μg/kg cut-off value 100 for qualitative range 4.125–82.397 quantitative detection. addition, test results real samples were consistent quantification using microbiological methods, coefficient variation less than 10%, showing good accuracy stability, confirming that suitable analysis powder samples. (mAbs) against VB12, fluorescence converting biological signals into optical signals.

Language: Английский

Citations

0

Intra-Mesopore Immunoassay Based on Core–Shell Structured Magnetic Hierarchically Porous ZIFs DOI

Fan Xia,

Fuzhong Liu,

Yingjun Yang

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

It is crucial yet challenging to sensitively quantify low-abundance biomarkers in blood for early screening and diagnosis of various diseases. Herein, an analytical model intra-mesopore immunoassay (IMIA) was proposed, which competent examine at the femtomolar level. The success rooted design innovative superparamagnetic core-shell structure with Fe3O4 nanoparticles (NPs) core hierarchically porous zeolitic imidazolate frameworks as a shell (Fe3O4@HPZIF-8), achieved through soft-template directed self-assembly coupled confinement growth mechanism. Such unique configuration conceptualized IMIA where HPZIF-8 served solid carrier cover capture antibodies while assisted its rapid separation. large pore channels not only provided stable microenvironment maintain recognition ability captured but also enhanced their coating density, thus promoting probability capturing binding target antigens, significantly improving (IA) sensitivity. practical clinic IA cTnI (Cardiac Troponin I, biomarker acute myocardial infarction (AMI)) human serums exemplified. developed could accurately slight fluctuations concentrations AMI patients different stages after symptom onset more than 100-fold enhancement limit detection (LOD) comparison conventional plate-based enzyme-linked immunosorbent assay (ELISA). high sensitivity makes it powerful tool accurate diseases by altering type primary antibody.

Language: Английский

Citations

0

Cross-material synergies of carbon nanomaterials, MOFs, and COFs: Innovative approaches for sustainable environmental remediation and resource recovery DOI Creative Commons
Prathmesh Bhadane, Swaroop Chakraborty

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 535, P. 216669 - 216669

Published: March 29, 2025

Language: Английский

Citations

0

In-Situ Synthesized Porphyrinic Zirconium Mof-Derived Multilayered Nanocomposite Probe for Ultra-Sensitive Immunosensor DOI
Xiangrui Li, H. Lv,

Xinyu Zhang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0

One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn DOI Creative Commons

Yinjun Li,

Hua Ding, Ziyu Wang

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 313 - 313

Published: May 14, 2025

Lateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages cost-effectiveness and operational simplicity. In this work, Eu metal-organic frameworks (MOFs) were introduced LFIA as method characterized by high stability low interference. Key research objectives included strong fluorescence, ease labeling, utilization fluorescent probes. Eu-MOFs synthesized one step via hydrothermal method, exhibiting fluorescence lifetime 163 μs spherical particles with diameters ranging from 250 400 nm. These conditions fulfill characteristics requirements LFIA. exploit porous nature MOFs mitigate drawbacks associated complex crosslinking agents. This enables antibody proteins be cross-linked merely upon contact, thereby simplifying process. A time-resolved was developed utilizing aflatoxin B1 (AFB1) corn, achieving limit (LOD, IC10) 0.149 ng/mL. The accuracy reliability Eu-MOFs-LFIA validated through comparisons spiked concentrations during spiking blind sample analyses, verification conducted using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS). Furthermore, testing real samples demonstrated that can effectively facilitate AFB1 corn.

Language: Английский

Citations

0

Bimetallic Nanoparticles as Electrochemical Labels in Immunosensors for the Detection of Biomarkers of Clinical Interest DOI Creative Commons
David Valero‐Calvo, Francisco J. Garcı́a Alonso, Alfredo de la Escosura‐Muñiz

et al.

Analysis & Sensing, Journal Year: 2024, Volume and Issue: 4(5)

Published: May 17, 2024

Abstract Electrochemical immunosensors have emerged in the last years as outstanding analytical systems for detection of analytes clinical interest. As alternative to traditional enzymatic labels, use nanoparticles and especially bimetallic ones has gained increased attention thanks their advantages related higher simplicity, stability sensitivity offered. Main routes such nanoparticle labels are based on i) dissolution into corresponding metal ions followed by voltammetric detection; ii) taking advantage electrocatalytic effect metals towards secondary reactions; iii) electrochemiluminescence properties.

Language: Английский

Citations

2

Introduction and general aspects of metal-organic frameworks and other novel reticular materials DOI
Jorge Pasán, María J. Trujillo‐Rodríguez, Verónica Pino

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 3 - 21

Published: Jan. 1, 2024

Language: Английский

Citations

2

Pyrroloquinoline quinone exhibiting peroxidase-mimicking property for colorimetric assays DOI
Ning Xia,

Fengli Gao,

Gang Liu

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1335, P. 343468 - 343468

Published: Nov. 22, 2024

Language: Английский

Citations

2