Freeze-Driven Adsorption of Oligonucleotides with polyA-Anchors on Au@Pt Nanozyme DOI Open Access

Nikita E. Lapshinov,

С. М. Придворова,

Anatoly V. Zherdev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10108 - 10108

Published: Sept. 20, 2024

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation remarkable catalytic properties. This study aimed explore the freeze–thaw method functionalizing with oligonucleotides featuring a polyadenine anchor. Spherical gold nanoparticles ([Au]NPs) were synthesized subsequently used as seeds produce urchin-like Au@Pt ([Au@Pt]NPs) an average diameter 29.8 nm. The conjugated series non-thiolated DNA oligonucleotides, each consisting three parts: 5′-polyadenine anchor (An, n = 3, 5, 7, 10; triple-branched A3, or A5), random sequence 23 nucleotides, linear polyT block seven deoxythymine residues. resulting conjugates characterized using transmission electron microscopy, spectroscopy, dynamic light scattering, emission detection fluorescent label at 3′-end oligonucleotide. stability was found depend on type oligonucleotide, decreased in row [Au@Pt]NP A7 > A5 3A3 3A5 A10 A3 anchors. These [Au@Pt]NP–oligonucleotide further evaluated lateral flow test strips assess fluorescein-specific binding peroxidase-like activity. Conjugates A5, A7, anchors showed highest levels signals bound labels strips, exceeding sensitivity by up nine times. findings hold significant potential broad application bioanalytical systems.

Language: Английский

FeMo6 integrated covalent organic frameworks: Peroxidase-mimetic colorimetric biosensors for multivariate sensing hydrogen peroxide and ascorbic acid in serum and beverages DOI
Xinyu Han,

Ji-hong Zhou,

Yunjie Li

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 479, P. 143727 - 143727

Published: March 6, 2025

Language: Английский

Citations

0
Development of a ZnNiMOF@CNT-based MIP electrochemical sensor: Toward the selective detection of creatinine in urine and saliva DOI
Shuang Yang, Haifeng Gao, Yukui Tong

et al.

Electrochimica Acta, Journal Year: 2025, Volume and Issue: 514, P. 145665 - 145665

Published: Jan. 5, 2025

Language: Английский

Citations

0
Pt@ZnCo2O4 Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection DOI Creative Commons

Shu-Ju Liao,

Wei Deng, Feng Yang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(1), P. 187 - 187

Published: Jan. 5, 2025

Compared to natural enzymes, the development of efficient artificial simulated such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo2O4 microspheres carriers synthesize Pt-doped composites different amounts using a one-pot method. The morphology structure synthesized were characterized XRD, SEM, BET, FT-IR, XPS, Zeta potential techniques. It was found that Pt0 adhered well surface microspheres, 12.5% Pt doped ratio exhibiting abundant oxygen vacancies, excellent substrate affinity, peroxidase-like activity. Using fluorescent probes electrochemical methods, mechanism has been explored Pt@ZnCo2O4 can accelerate electron transfer between H2O2 3,3',5,5'-tetramethylbenzidine (TMB). Based optimal loading Pt@ZnCo2O4, colorimetric sensor for visual detection L-cysteine (L-Cys) constructed, wide linear range 0.1~50 µM low limit 0.0163 µM. possesses selectivity, reusability, usage which be applied determination L-Cys in health product capsules recovery rates 96.9%~103.7% RSD 1.07%~6.50%. This work broadens application prospects spinel field biological analysis also provides inspiration new enzymes.

Language: Английский

Citations

0
Advances and Future Trends in Nanozyme-Based SERS Sensors for Food Safety, Environmental and Biomedical Applications DOI Open Access
Xingyu Wang, Xuemei Tang,

Chengzhen Ji

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 709 - 709

Published: Jan. 15, 2025

Nanozymes, a kind of nanoparticles with enzyme-mimicking activities, have attracted considerable attention due to their robust catalytic properties, ease preparation, and resistance harsh conditions. By combining nanozymes surface-enhanced Raman spectroscopy (SERS) technology, highly sensitive selective sensors been developed. These are capable detecting wide range analytes, such as foodborne toxins, environmental pollutants, biomedical markers. This review provides an overview recent advancements in the synthesis surface modification nanozymes, highlighting ability mimic multiple enzymes enhance performance. In addition, we explore development applications nanozyme-based SERS food contaminants, The concludes perspectives challenges facing field, involving need for deeper understanding nanozyme principles mechanisms, standardized systems characterization, engineering tailored properties specific applications. Finally, discuss potential integrating various techniques create multi-modal detection platforms, paving way next generation analytical tools fields safety, monitoring, diagnostics.

Language: Английский

Citations

0
Design and Application of Flower-like Iron Single-Atom Nanozymes for Sensitive Colorimetric Detection of Caffeic Acid DOI
Ling Jiang,

Yuxiu Fan,

Yingying Li

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Language: Английский

Citations

0
Signal “Off–On Model’’ for Colorimetric Sensing Proanthocyanidin B2 Achieved by the Organic Solvent-Processed Amorphous BiVO4 Nanozyme DOI

Fan Lu,

Yun-Yun Wu,

Sheng Feng

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Here, we report a "off-on model"-based colorimetric sensor without the assistance of H2O2, achieved by organic solvent-processed amorphous BiVO4 nanoparticles favorable for large-scale manufacturing. The material beyond traditional crystalline nanozymes has both vanadium vacancy (Vv) and oxygen (Ov), as well single oxidase-like activity with hydroxyl radical (•OH) superoxide anion (O2•-) electron acceptors. Despite high enzymatic performance, dual-defect-rich preserves good long-term in either buffer solution or solid state. specially designed is capable accelerating TMB oxidation presence polyphenols, thus leading to an interesting signal-intensified response. This probably due coordination ortho dihydroxyl group polyphenols catalyst replacing surface-adsorbed NO3-, which results faster charge transfer between substrate and, thus, more rapid depletion radicals oxidation. Based on these findings, platform proanthocyanidin (PAC) B2 established limit detection (LOD) low 65 nM specificity. shows higher sensitivity than standard Porter method (LOD: 0.29 μM) comparable accuracy when analyzing PAC commercially available grape seed capsules, quantitative recoveries varying from 104.0 108.3% relative deviations ranging 1.7 5.7%. To date, this only nanozyme-based chemical that active signal model" reducing polyphenols. Also, seems quite general, it can be used sensing series specific addition B2.

Language: Английский

Citations

0
Exploration of cobalt-based colorimetric aptasensing of zearalenone in cereal products: Enhanced performance of Au/CoOOH nanozyme DOI Creative Commons
Qi Sun, Miaomiao Ma, Zhijuan Li

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117700 - 117700

Published: March 1, 2025

Language: Английский

Citations

0
Recent advances in multifunctional nanomaterials enhanced immunochromatographic assays DOI

Yanna Shao,

Sicheng Xiong,

Mingzhu Huang

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118013 - 118013

Published: Oct. 1, 2024

Language: Английский

Citations

3
Bibliometric Analysis and Network Visualization of Nanozymes for Microbial Theranostics in the Last Decade DOI
Hamza Ettadili, Caner Vural

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Language: Английский

Citations

1
Freeze-Driven Adsorption of Oligonucleotides with polyA-Anchors on Au@Pt Nanozyme DOI Open Access

Nikita E. Lapshinov,

С. М. Придворова,

Anatoly V. Zherdev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10108 - 10108

Published: Sept. 20, 2024

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation remarkable catalytic properties. This study aimed explore the freeze–thaw method functionalizing with oligonucleotides featuring a polyadenine anchor. Spherical gold nanoparticles ([Au]NPs) were synthesized subsequently used as seeds produce urchin-like Au@Pt ([Au@Pt]NPs) an average diameter 29.8 nm. The conjugated series non-thiolated DNA oligonucleotides, each consisting three parts: 5′-polyadenine anchor (An, n = 3, 5, 7, 10; triple-branched A3, or A5), random sequence 23 nucleotides, linear polyT block seven deoxythymine residues. resulting conjugates characterized using transmission electron microscopy, spectroscopy, dynamic light scattering, emission detection fluorescent label at 3′-end oligonucleotide. stability was found depend on type oligonucleotide, decreased in row [Au@Pt]NP A7 > A5 3A3 3A5 A10 A3 anchors. These [Au@Pt]NP–oligonucleotide further evaluated lateral flow test strips assess fluorescein-specific binding peroxidase-like activity. Conjugates A5, A7, anchors showed highest levels signals bound labels strips, exceeding sensitivity by up nine times. findings hold significant potential broad application bioanalytical systems.

Language: Английский

Citations

0