Lung tumor organoids migrate as cell clusters containing cancer stem cells under hypoxic condition

Biology of the Cell, Journal Year: 2025, Volume and Issue: 117(2)

Published: Feb. 1, 2025

Tumor hypoxia reshapes the microenvironment, driving progression, invasion, metastasis, and therapy resistance. Patient-derived tumor organoids, formed under three-dimensional conditions, preserve cellular heterogeneity nutrient gradients, making them ideal for studying hypoxia-induced responses. This study examines changes in lung organoids from two patients, focusing on tumor-associated markers, stem cell migration capabilities. Our findings demonstrate that distinctively modulates expression of cancer markers thyroid transcription factor-1, cytokeratin 7, ΔNP63 variant. Hypoxia also induces upregulation cell-associated resulting an increased proportion cells. Furthermore, hypoxic exhibit unique migratory behavior upon reoxygenation, driven by epithelial-mesenchymal transition elevated matrix metalloproteinases 7 9, indicating their enhanced invasive potential. These highlight value as models hypoxia's complex role cancer. significantly growth, stemness, behavior, providing critical insights into progression

Language: Английский

Epigenetic Biomarkers as a New Diagnostic Tool in Bladder Cancer—From Early Detection to Prognosis

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(23), P. 7159 - 7159

Published: Nov. 26, 2024

Bladder cancer (BC) currently ranks as the 9th most common worldwide. It is characterised by very high rates of recurrence and metastasis. Most cases BC are urothelial origin, due to its ability penetrate muscle tissue, divided into non-muscle-invasive (NMIBC) muscle-invasive (MIBC). The current diagnosis still based primarily on invasive cystoscopy, which an expensive method that carries a risk various complications. Urine sediment cytology often used complementary test, biggest drawback low sensitivity concerning detection at early stages, crucial for prompt implementation appropriate treatment. Therefore, there great need develop innovative diagnostic techniques would enable accurate prognosis BC. Great potential in this regard shown epigenetic changes, possible observe long before onset clinical symptoms disease. In addition, these changes can be detected readily available biological material, such urine or blood, indicating possibility constructing non-invasive tests. Over past few years, many studies have emerged using alterations novel prognostic biomarkers This review provides update promising DNA methylation expression levels selected non-coding RNAs (ncRNAs), taking account latest literature data.

Language: Английский